Tcf7l2 (NM_001142920) Mouse Tagged ORF Clone

CAT#: MR224589

Tcf7l2 (Myc-DDK-tagged) - Mouse transcription factor 7-like 2, T-cell specific, HMG-box (Tcf7l2), transcript variant 4

Product Images

Circular map for MR224589

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tcf7l2
• Synonyms: Tcf-4; Tcf4; TCF4B; TCF4E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224589 representing NM_001142920
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCAGCTGAACGGCGGTGGAGGAGATGACCTAGGCGCTAACGACGAGCTGATCTCCTTCAAAGACG
  AAGGCGAGCAGGAGGAGAAGAACTCGGAAAACTCCTCGGCGGAAAGGGATTTAGCCGATGTCAAGTCCTC
  GCTGGTCAATGAATCAGAGACGAATCAAAACAGCTCCTCCGATTCCGAGGCGGAAAGACGGCCTCCGCCT
  CGCTCCGAAAGTTTCCGAGATAAATCCCGGGAAAGTTTGGAAGAAGCGGCCAAGAGGCAAGATGGAGGGC
  TCTTTAAGGGGCCACCGTATCCCGGCTACCCCTTCATCATGATCCCCGACCTGACGAGCCCCTACCTCCC
  CAACGGATCGCTCTCGCCCACCGCCCGAACCCTCCATTTTCAATCCGGCAGCACTCATTACTCTGCGTAC
  AAAACGATTGAACACCAGATTGCAATTCAGTATCTTCAGATGAAATGGCCACTGCTTGATGTCCAAGCAG
  GAAGCCTCCAGAGCAGACAAACCCTCAAGGATGCTCGTTCGCCGTCGCCAGCACACATCGTTTCGAACAA
  AGTACCGGTGGTGCAACACCCCCACCATGTCCACCCACTCACGCCTCTCATCACGTACAGCAATGAACAC
  TTCACCCCGGGAAATCCACCTCCGCACTTACCAGCTGACGTAGACCCCAAAACAGGAATCCCAAGGCCTC
  CGCACCCTCCAGATATCTCTCCATATTACCCGCTGTCGCCCGGCACCGTAGGACAAATCCCCCATCCGCT
  AGGATGGCAAGGTCAGCCTGTGTACCCAATCACGACAGGAGGATTCAGACACCCCTACCCCACAGCGCTG
  ACAGTCAACGCATCTATGTCTAGCTTTCTGTCCTCTAGGTTCCCTCCCCATATGGTCCCTCCCCATCACA
  CTCTGCACACGACCGGCATCCCTCACCCGGCCATCGTCACACCGACAGTCAAGCAGGAATCCTCCCAGAG
  TGACGTCGGCTCACTCCACAGCTCAAAGCATCAGGACTCCAAAAAGGAAGAAGAGAAGAAGAAGCCCCAC
  ATAAAGAAGCCCCTTAATGCATTCATGTTGTATATGAAAGAGATGAGAGCGAAGGTGGTGGCCGAATGCA
  CATTGAAAGAGAGTGCAGCCATCAACCAGATTCTCGGGCGCAGGTGGCACGCCCTGTCCAGGGAAGAACA
  GGCAAAATACTACGAGCTGGCCCGGAAGGAACGACAGCTTCACATGCAGCTGTACCCTGGCTGGTCTGCA
  CGGGATAACTATGAGAAAAAAAAAGTGCGTTCGCTACATACAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224589 representing NM_001142920
  Red=Cloning site Green=Tags(s)
  
  MPQLNGGGGDDLGANDELISFKDEGEQEEKNSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPP
  RSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTLHFQSGSTHYSAY
  KTIEHQIAIQYLQMKWPLLDVQAGSLQSRQTLKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEH
  FTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWQGQPVYPITTGGFRHPYPTAL
  TVNASMSSFLSSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPH
  IKKPLNAFMLYMKEMRAKVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSA
  RDNYEKKKVRSLHTR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142920
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142920.2
• RefSeq Size: 4037 bp
• RefSeq ORF: 1308 bp
• Locus ID: 21416
• Cytogenetics: 19 51.59 cM
• MW: 49.1 kDa
• Gene Summary: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif CCTTTGATC in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1 (By similarity). Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.[UniProtKB/Swiss-Prot Function]