Mllt3 (NM_027326) Mouse Tagged ORF Clone

CAT#: MR224561

Mllt3 (Myc-DDK-tagged) - Mouse myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 3 (Mllt3), transcript variant 1

Product Images

Circular map for MR224561

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mllt3
• Synonyms: 2210011H10Rik; 2610012I03Rik; 3830408D16Rik; Af9; D4Ertd321e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224561 representing NM_027326
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGCTCGTGTGCCGTGCAGGTGAAGCTGGAGCTGGGGCACCGCGCCCAGGTGAGGAAGAAACCCA
  CAGTGGAGGGCTTCACCCACGACTGGATGGTGTTCGTGCGCGGCCCGGAGCACAGTAACATTCAGCACTT
  TGTGGAGAAAGTCGTCTTCCACTTGCACGAAAGCTTCCCTAGGCCAAAAAGAGTGTGCAAAGACCCACCT
  TACAAGGTAGAAGAGTCCGGGTACGCTGGCTTCATCTTGCCAATTGAAGTTTATTTTAAAAACAAGGAAG
  AACCTAAGAAAGTCCGCTTTGATTATGACTTATTCCTGCATCTTGAAGGCCACCCACCAGTGAATCACCT
  CCGCTGTGAGAAACTAACTTTCAATAACCCCACGGAGGACTTTAGAAGAAAGTTGCTGAAGGCAGGAGGG
  GACCCTAACAGGAGCATTCATACCAGCAGTAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCA
  GCAGTAGTAGTAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAG
  CAGCAGTAGTACCAGTTTTTCAAAGCCTCACAAATTAATGAAGGAGCACAAGGAAAAACCTTCTAAGGAC
  TCCAGAGAACATAAAAGTGCCTTCAAAGAACCTTCCAGGGATCACAACAAATCTTCCAAAGACTCCTCCA
  AGAAACCCAAAGAAAACAAACCACTGAAAGAAGAAAAAATAGTTCCTAAGATGGCCTTCAAGGAACCTAA
  ACCCATGTCAAAAGAGCCAAAAGCAGACAGTAACTTACTCACCGTCACCAGTGGGCAGCAGGACAAGAAG
  GCGCCCAGCAAAAGGCCCCCTGCTTCAGATTCTGAAGAACTCTCAGCCAAAAAAAGGAAAAAGAGTAGCT
  CGGAGGCTTTATTTAAAAGTTTTTCTAGTGCGCCCCCACTGATACTCACTTGTTCTGCTGACAAAAAACA
  GATAAAAGATAAATCTCATGTCAAGATGGGAAAGGTCAAAATTGAGAGTGAGACTTCAGAGAAGAAGAAG
  TCAATGTTGCCACCGTTTGATGACATCGTGGATCCCAATGACTCAGACGTGGAGGAGAACATGTCCTCGA
  AATCTGACTCAGAGCAACCCAGTCCTGCCAGCTCCAGCTCCAGTTCCAGCTCCAGCTTCACACCATCCCA
  GACCAGGCAACAAGGTCCTTTAAGGTCGATCATGAAAGATCTGCATTCTGATGACAACGAGGAGGAGTCT
  GATGAGGCAGAGGACAATGACAATGACTCGGAGATGGAAAGACCTGTAAATAGAGGAGGAAGCCGAAGTC
  GCAGGGTCAGCTTGAGTGACGGCAGTGACAGTGAAAGCAGCTCCGCCTCTTCCCCTCTCCATCATGAACC
  CCCGCCACCATTATTGAAAACTAACAACAACCAGATCCTTGAAGTGAAAAGTCCAATAAAACAAAGCAAA
  TCAGATAAGCAAATAAAGAATGGTGAATGTGACAAGGCATATCTAGATGAGTTGGTAGAGCTCCACAGAA
  GGTTAATGACACTGAGGGAACGACACATTCTGCAGCAGATCGTGAACCTTATAGAGGAAACTGGACACTT
  TCATATTACGAATACAACATTTGATTTTGATCTTTGCTCGCTGGACAAAACTACAGTCCGTAAATTACAG
  AGTTACCTGGAAACATCTGGAACATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224561 representing NM_027326
  Red=Cloning site Green=Tags(s)
  
  MASSCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPP
  YKVEESGYAGFILPIEVYFKNKEEPKKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKAGG
  DPNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSFSKPHKLMKEHKEKPSKD
  SREHKSAFKEPSRDHNKSSKDSSKKPKENKPLKEEKIVPKMAFKEPKPMSKEPKADSNLLTVTSGQQDKK
  APSKRPPASDSEELSAKKRKKSSSEALFKSFSSAPPLILTCSADKKQIKDKSHVKMGKVKIESETSEKKK
  SMLPPFDDIVDPNDSDVEENMSSKSDSEQPSPASSSSSSSSSFTPSQTRQQGPLRSIMKDLHSDDNEEES
  DEAEDNDNDSEMERPVNRGGSRSRRVSLSDGSDSESSSASSPLHHEPPPPLLKTNNNQILEVKSPIKQSK
  SDKQIKNGECDKAYLDELVELHRRLMTLRERHILQQIVNLIEETGHFHITNTTFDFDLCSLDKTTVRKLQ
  SYLETSGTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027326
• ORF Size: 1707 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027326.3, NP_081602.3
• RefSeq Size: 6129 bp
• RefSeq ORF: 1710 bp
• Locus ID: 70122
• UniProt ID: A2AM29
• Cytogenetics: 4 41.06 cM
• MW: 63.8 kDa
• Gene Summary: Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated at 'Lys-9' (H3K9ac), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones.[UniProtKB/Swiss-Prot Function]