Kdr (NM_010612) Mouse Tagged ORF Clone

CAT#: MR224504

Kdr (Myc-DDK-tagged) - Mouse kinase insert domain protein receptor (Kdr)

Product Images

Circular map for MR224504

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kdr
• Synonyms: 6130401C07; Flk-1; Flk1; Krd-1; Ly73; sVEGFR-2; VEGFR-2; VEGFR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224504 representing NM_010612
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCAAGGCGCTGCTAGCTGTCGCTCTGTGGTTCTGCGTGGAGACCCGAGCCGCCTCTGTGGGTT
  TGCCTGGCGATTTTCTCCATCCCCCCAAGCTCAGCACACAGAAAGACATACTGACAATTTTGGCAAATAC
  AACCCTTCAGATTACTTGCAGGGGACAGCGGGACCTGGACTGGCTTTGGCCCAATGCTCAGCGTGATTCT
  GAGGAAAGGGTATTGGTGACTGAATGCGGCGGTGGTGACAGTATCTTCTGCAAAACACTCACCATTCCCA
  GGGTGGTTGGAAATGATACTGGAGCCTACAAGTGCTCGTACCGGGACGTCGACATAGCCTCCACTGTTTA
  TGTCTATGTTCGAGATTACAGATCACCATTCATCGCCTCTGTCAGTGACCAGCATGGCATCGTGTACATC
  ACCGAGAACAAGAACAAAACTGTGGTGATCCCCTGCCGAGGGTCGATTTCAAACCTCAATGTGTCTCTTT
  GCGCTAGGTATCCAGAAAAGAGATTTGTTCCGGATGGAAACAGAATTTCCTGGGACAGCGAGATAGGCTT
  TACTCTCCCCAGTTACATGATCAGCTATGCCGGCATGGTCTTCTGTGAGGCAAAGATCAATGATGAAACC
  TATCAGTCTATCATGTACATAGTTGTGGTTGTAGGATATAGGATTTATGATGTGATTCTGAGCCCCCCGC
  ATGAAATTGAGCTATCTGCCGGAGAAAAACTTGTCTTAAATTGTACAGCGAGAACAGAGCTCAATGTGGG
  GCTTGATTTCACCTGGCACTCTCCACCTTCAAAGTCTCATCATAAGAAGATTGTAAACCGGGATGTGAAA
  CCCTTTCCTGGGACTGTGGCGAAGATGTTTTTGAGCACCTTGACAATAGAAAGTGTGACCAAGAGTGACC
  AAGGGGAATACACCTGTGTAGCGTCCAGTGGACGGATGATCAAGAGAAATAGAACATTTGTCCGAGTTCA
  CACAAAGCCTTTTATTGCTTTCGGTAGTGGGATGAAATCTTTGGTGGAAGCCACAGTGGGCAGTCAAGTC
  CGAATCCCTGTGAAGTATCTCAGTTACCCAGCTCCTGATATCAAATGGTACAGAAATGGAAGGCCCATTG
  AGTCCAACTACACAATGATTGTTGGCGATGAACTCACCATCATGGAAGTGACTGAAAGAGATGCAGGAAA
  CTACACGGTCATCCTCACCAACCCCATTTCAATGGAGAAACAGAGCCACATGGTCTCTCTGGTTGTGAAT
  GTCCCACCCCAGATCGGTGAGAAAGCCTTGATCTCGCCTATGGATTCCTACCAGTATGGGACCATGCAGA
  CATTGACATGCACAGTCTACGCCAACCCTCCCCTGCACCACATCCAGTGGTACTGGCAGCTAGAAGAAGC
  CTGCTCCTACAGACCCGGCCAAACAAGCCCGTATGCTTGTAAAGAATGGAGACACGTGGAGGATTTCCAG
  GGGGGAAACAAGATCGAAGTCACCAAAAACCAATATGCCCTGATTGAAGGAAAAAACAAAACTGTAAGTA
  CGCTGGTCATCCAAGCTGCCAACGTGTCAGCGTTGTACAAATGTGAAGCCATCAACAAAGCGGGACGAGG
  AGAGAGGGTCATCTCCTTCCATGTGATCAGGGGTCCTGAAATTACTGTGCAACCTGCTGCCCAGCCAACT
  GAGCAGGAGAGTGTGTCCCTGTTGTGCACTGCAGACAGAAATACGTTTGAGAACCTCACGTGGTACAAGC
  TTGGCTCACAGGCAACATCGGTCCACATGGGCGAATCACTCACACCAGTTTGCAAGAACTTGGATGCTCT
  TTGGAAACTGAATGGCACCATGTTTTCTAACAGCACAAATGACATCTTGATTGTGGCATTTCAGAATGCC
  TCTCTGCAGGACCAAGGCGACTATGTTTGCTCTGCTCAAGATAAGAAGACCAAGAAAAGACATTGCCTGG
  TCAAACAGCTCATCATCCTAGAGCGCATGGCACCCATGATCACCGGAAATCTGGAGAATCAGACAACAAC
  CATTGGCGAGACCATTGAAGTGACTTGCCCAGCATCTGGAAATCCTACCCCACACATTACATGGTTCAAA
  GACAACGAGACCCTGGTAGAAGATTCAGGCATTGTACTGAGAGATGGGAACCGGAACCTGACTATCCGCA
  GGGTGAGGAAGGAGGATGGAGGCCTCTACACCTGCCAGGCCTGCAATGTCCTTGGCTGTGCAAGAGCGGA
  GACGCTCTTCATAATAGAAGGTGCCCAGGAAAAGACCAACTTGGAAGTCATTATCCTCGTCGGCACTGCA
  GTGATTGCCATGTTCTTCTGGCTCCTTCTTGTCATTGTCCTACGG[AT]CCGTTAAGCGGGCCAATGAAG
  GGGAACTGAAGACAGGCTACTTGTCTATTGTCATGGATCCAGATGAATTGCCCTTGGATGAGCGCTGTGA
  ACGCTTGCCTTATGATGCCAGCAAGTGGGAATTCCCCAGGGACCGGCTGAAACTAGGAAAACCTCTTGGC
  CGCGGTGCCTTCGGCCAAGTGATTGAGGCAGACGCTTTTGGAATTGACAAGACAGCGACTTGCAAAACAG
  TAGCCGTCAAGATGTTGAAAGAAGGAGCAACACACAGCGAGCATCGAGCCCTCATGTCTGAACTCAAGAT
  CCTCATCCACATTGGTCACCATCTCAATGTGGTGAACCTCCTAGGCGCCTGCACCAAGCCGGGAGGGCCT
  CTCATGGTGATTGTGGAATTCTGCAAGTTTGGAAACCTATCAACTTACTTACGGGGCAAGAGAAATGAAT
  TTGTTCCCTATAAGAGCAAAGGGGCACGCTTCCGCCAGGGCAAGGACTACGTTGGGGAGCTCTCCGTGGA
  TCTGAAAAGACGCTTGGACAGCATCACCAGCAGCCAGAGCTCTGCCAGCTCAGGCTTTGTTGAGGAGAAA
  TCGCTCAGTGATGTAGAGGAAGAAGAAGCTTCTGAAGAACTGTACAAGGACTTCCTGACCTTGGAGCATC
  TCATCTGTTACAGCTTCCAAGTGGCTAAGGGCATGGAGTTCTTGGCATCAAGGAAGTGTATCCACAGGGA
  CCTGGCAGCACGAAACATTCTCCTATCGGAGAAGAATGTGGTTAAGATCTGTGACTTCGGCTTGGCCCGG
  GACATTTATAAAGACCCGGATTATGTCAGAAAAGGAGATGCCCGACTCCCTTTGAAGTGGATGGCCCCGG
  AAACCATTTTTGACAGAGTATACACAATTCAGAGCGATGTGTGGTCTTTCGGTGTGTTGCTCTGGGAAAT
  ATTTTCCTTAGGTGCCTCCCCATACCCTGGGGTCAAGATTGATGAAGAATTTTGTAGGAGATTGAAAGAA
  GGAACTAGAATGCGGGCTCCTGACTACACTACCCCAGAAATGTACCAGACCATGCTGGACTGCTGGCATG
  AGGACCCCAACCAGAGACCCTCGTTTTCAGAGTTGGTGGAGCATTTGGGAAACCTCCTGCAAGCAAATGC
  GCAGCAGGATGGCAAAGACTATATTGTTCTTCCAATGTCAGAGACACTGAGCATGGAAGAGGATTCTGGA
  CTCTCCCTGCCTACCTCACCTGTTTCCTGTATGGAGGAAGAGGAAGTGTGCGACCCCAAATTCCATTATG
  ACAACACAGCAGGAATCAGTCATTATCTCCAGAACAGTAAGCGAAAGAGCCGGCCAGTGAGTGTAAAAAC
  ATTTGAAGATATCCCATTGGAGGAACCAGAAGTAAAAGTGATCCCAGATGACAGCCAGACAGACAGTGGG
  ATGGTCCTTGCATCAGAAGAGCTGAAAACTCTGGAAGACAGGAACAAATTATCTCCATCTTTTGGTGGAA
  TGATGCCCAGTAAAAGCAGGGAGTCTGTGGCCTCGGAAGGCTCCAACCAGACCAGTGGCTACCAGTCTGG
  GTATCACTCAGATGACACAGACACCACCGTGTACTCCAGCGACGAGGCAGGACTTTTAAAGATGGTGGAT
  GCTGCAGTTCACGCTGACTCAGGGACCACACTGCGCTCACCTCCTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224504 representing NM_010612
  Red=Cloning site Green=Tags(s)
  
  MESKALLAVALWFCVETRAASVGLPGDFLHPPKLSTQKDILTILANTTLQITCRGQRDLDWLWPNAQRDS
  EERVLVTECGGGDSIFCKTLTIPRVVGNDTGAYKCSYRDVDIASTVYVYVRDYRSPFIASVSDQHGIVYI
  TENKNKTVVIPCRGSISNLNVSLCARYPEKRFVPDGNRISWDSEIGFTLPSYMISYAGMVFCEAKINDET
  YQSIMYIVVVVGYRIYDVILSPPHEIELSAGEKLVLNCTARTELNVGLDFTWHSPPSKSHHKKIVNRDVK
  PFPGTVAKMFLSTLTIESVTKSDQGEYTCVASSGRMIKRNRTFVRVHTKPFIAFGSGMKSLVEATVGSQV
  RIPVKYLSYPAPDIKWYRNGRPIESNYTMIVGDELTIMEVTERDAGNYTVILTNPISMEKQSHMVSLVVN
  VPPQIGEKALISPMDSYQYGTMQTLTCTVYANPPLHHIQWYWQLEEACSYRPGQTSPYACKEWRHVEDFQ
  GGNKIEVTKNQYALIEGKNKTVSTLVIQAANVSALYKCEAINKAGRGERVISFHVIRGPEITVQPAAQPT
  EQESVSLLCTADRNTFENLTWYKLGSQATSVHMGESLTPVCKNLDALWKLNGTMFSNSTNDILIVAFQNA
  SLQDQGDYVCSAQDKKTKKRHCLVKQLIILERMAPMITGNLENQTTTIGETIEVTCPASGNPTPHITWFK
  DNETLVEDSGIVLRDGNRNLTIRRVRKEDGGLYTCQACNVLGCARAETLFIIEGAQEKTNLEVIILVGTA
  VIAMFFWLLLVIVLRXXVKRANEGELKTGYLSIVMDPDELPLDERCERLPYDASKWEFPRDRLKLGKPLG
  RGAFGQVIEADAFGIDKTATCKTVAVKMLKEGATHSEHRALMSELKILIHIGHHLNVVNLLGACTKPGGP
  LMVIVEFCKFGNLSTYLRGKRNEFVPYKSKGARFRQGKDYVGELSVDLKRRLDSITSSQSSASSGFVEEK
  SLSDVEEEEASEELYKDFLTLEHLICYSFQVAKGMEFLASRKCIHRDLAARNILLSEKNVVKICDFGLAR
  DIYKDPDYVRKGDARLPLKWMAPETIFDRVYTIQSDVWSFGVLLWEIFSLGASPYPGVKIDEEFCRRLKE
  GTRMRAPDYTTPEMYQTMLDCWHEDPNQRPSFSELVEHLGNLLQANAQQDGKDYIVLPMSETLSMEEDSG
  LSLPTSPVSCMEEEEVCDPKFHYDNTAGISHYLQNSKRKSRPVSVKTFEDIPLEEPEVKVIPDDSQTDSG
  MVLASEELKTLEDRNKLSPSFGGMMPSKSRESVASEGSNQTSGYQSGYHSDDTDTTVYSSDEAGLLKMVD
  AAVHADSGTTLRSPPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010612
• ORF Size: 4038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010612.2, NP_034742.2
• RefSeq Size: 5464 bp
• RefSeq ORF: 4038 bp
• Locus ID: 16542
• UniProt ID: P35918
• Cytogenetics: 5 40.23 cM
• MW: 150.9 kDa
• Gene Summary: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.[UniProtKB/Swiss-Prot Function]