Mta3 (NM_054082) Mouse Tagged ORF Clone

CAT#: MR224341

Mta3 (Myc-DDK-tagged) - Mouse metastasis associated 3 (Mta3), transcript variant 2

Product Images

Circular map for MR224341

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mta3
• Synonyms: 1110002J22Rik; mKIAA1266
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224341 representing NM_054082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCAACATGTACCGGGTCGGAGACTACGTTTATTTTGAGAATTCCTCCAGCAACCCGTACCTCA
  TCAGACGGATAGAGGAGCTCAACAAGACTGCAAGTGGCAATGTGGAAGCAAAAGTCGTCTGCTTTTACAG
  AAGACGGGACATCTCCAACACGCTTATCATGCTCGCCGACAAGCATGCTAAAGAAACTGAGGAGGAGTCG
  GAGACGCCAGTTGAGGCCGACTTGACGGAGAAGCAGAAGCACCAGCTGAAACACAGGGAGCTCTTTCTGT
  CGCGCCAGTACGAGTCACTGCCTGCGACACATATCAGGGGGAAGTGCAGCGTGGCCCTGCTGAATGAGAC
  AGAATCCGTGTTGTCATACCTTGACAAGGAGGATACCTTCTTCTACTCACTGGTATACGACCCTTCAGTG
  AAAACATTATTGGCTGACAAAGGTGAAATCAGAGTGGGCCCAAAGTACCAAGCTGACATTCCAGACATGC
  TACCAGAAGACTCAGATGAGAGGGAACAATCAAAATTGGAAGTTAAGGTTTGGGACCCCAATAGCCCACT
  TACGGATCGACAGATTGACCAGTTTTTAGTTGTAGCCCGTGCCGTGGGAACATTTGCCCGAGCCCTGGAC
  TGCAGCAGCTCCGTGAGGCAACCCAGCCTTCACATGAGTGCAGCCGCAGCCTCCCGAGACATCACCTTGT
  TTCATGCCATGGACACACTGTACAGGCACGGCTATGACCTCAGCAGCGCCATCAGTGTGCTGGTGCCGCT
  CGGAGGGCCAGTCCTCTGCAGGGACGAGATGGAGGAGTGGTCGGCCTCTGAAGCCTGCCTATTCGAAGAA
  GCACTGGAAAAATACGGCAAAGATTTCAACGACATTCGTCAGGACTTTCTCCCGTGGAAATCCTTGACTA
  GCATCATTGAATATTATTACATGTGGAAAACTACTGACAGATACGTGCAGCAGAAGCGCCTAAAGGCCGC
  AGAAGCCGAGAGCAAACTGAAACAAGTGTACATCCCGACTTACAAACCAAATCCCAACCAAATCTCCAGC
  AGCAACGGCAAGGCTGGCACTGTGAATGGAGCTGTGGGGACCCAGTTTCAGCCCCAGAGCGCCCTCCTAG
  GGCGAGCCTGCGAGAGCTGCTACGCCACACAGTCTCATCAGTGGTATTCCTGGGGCCCACCTAACATGCA
  GTGTAGACTCTGCGCGACCTGTTGGCTGTATTGGAAAAAGTATGGAGGTCTGAAAATGCCTACCCAGTCA
  GACGAAGAGAAGTCTCCCAGCCCGACTGCAGAGGACCCGCGCGCAAGAAGCCACATGTCCCGGCAGGCCT
  TGCAGGGCATGCCGGTCCGGAACACCGGAAGCCCCAAGTCTGCAGTGAAGACCCGCCAAGCTTTCTTCCT
  TCGTACTACGTATTTCACAAAAATTGCTCGTCAGGTCTGCAAAAGCACCCTGCGGCTGCGGCAGGCAGCG
  AGACGGCCGTTTGTTGCTATTAATTATGCTGCCATTAGGGCAGAATATGCAGACAGACATGCCGAACTTT
  CTGGAAGCCCACTGAAAAGCAGAAGCACCAGGAAACCTTTGTCGTGTATCATTGGGTATTTAGAGATCCA
  TCCCGCAAAGAAACCTAATGTAATTCGGTCTCCACCAAGCCTGCAAACTCCAGCTACCAAGAGGATGCTC
  GCCGCTCCGAATCACACATCTCTGAGCATTCTGGGGAAAAGAAACTACAGCCATCACAATGGTCTAGATG
  GGCCAGAACGATGGCTAAGCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224341 representing NM_054082
  Red=Cloning site Green=Tags(s)
  
  MAANMYRVGDYVYFENSSSNPYLIRRIEELNKTASGNVEAKVVCFYRRRDISNTLIMLADKHAKETEEES
  ETPVEADLTEKQKHQLKHRELFLSRQYESLPATHIRGKCSVALLNETESVLSYLDKEDTFFYSLVYDPSV
  KTLLADKGEIRVGPKYQADIPDMLPEDSDEREQSKLEVKVWDPNSPLTDRQIDQFLVVARAVGTFARALD
  CSSSVRQPSLHMSAAAASRDITLFHAMDTLYRHGYDLSSAISVLVPLGGPVLCRDEMEEWSASEACLFEE
  ALEKYGKDFNDIRQDFLPWKSLTSIIEYYYMWKTTDRYVQQKRLKAAEAESKLKQVYIPTYKPNPNQISS
  SNGKAGTVNGAVGTQFQPQSALLGRACESCYATQSHQWYSWGPPNMQCRLCATCWLYWKKYGGLKMPTQS
  DEEKSPSPTAEDPRARSHMSRQALQGMPVRNTGSPKSAVKTRQAFFLRTTYFTKIARQVCKSTLRLRQAA
  RRPFVAINYAAIRAEYADRHAELSGSPLKSRSTRKPLSCIIGYLEIHPAKKPNVIRSPPSLQTPATKRML
  AAPNHTSLSILGKRNYSHHNGLDGPERWLSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_054082
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_054082.2, NP_473423.1
• RefSeq Size: 2115 bp
• RefSeq ORF: 1776 bp
• Locus ID: 116871
• UniProt ID: Q924K8
• Cytogenetics: 17 E4
• MW: 67.5 kDa
• Gene Summary: Plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels. Contributes to transcriptional repression by BCL6 (By similarity).[UniProtKB/Swiss-Prot Function]