Nkx3-2 (NM_007524) Mouse Tagged ORF Clone

CAT#: MR224227

Nkx3 (Myc-DDK-tagged) - Mouse NK3 homeobox 2 (Nkx3-2)

Product Images

Circular map for MR224227

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nkx3-2
• Synonyms: Bapx1; Nkx-3.2; NKX3.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224227 representing NM_007524
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGCGCGGCAGCGGCACCTTGACGCCCTTCTCCATCCAGGCGATCCTCAACAAGAAAGAGGAGC
  GCGGCGGGTTGGCTACGCCGGAGGGGCGCCCGGCACCAGGGGGCACAGAGGTGGCGGTGACCGCGGCGCC
  CGCCGTCTGCTGCTGGCGGATCTTCGGGGAGACCGAGGCCGGTGCGCTGGGGGGCGCCGAGGACTCTCTT
  CTGGCATCTCCCGCCCGAACCAGAACAGCCGTGGGGCAGAGCGCAGAGAGTCCGGGAGGTTGGGACTCAG
  ACTCAGCCCTGAGCGAAGAGAACGAGGGCAGGAGGCGCTGCGCCGATGTCCCGGGAGCCAGTGGAACCGG
  CCGCGCCAGGGTGACCCTGGGCCTGGACCAGCCAGGCTGCGAGCTGCACGCCGCCAAGGACCTGGAGGAG
  GAAGCCCCGGTCCGCAGCGACAGCGAGATGTCAGCCAGCGTTTCAGGCGACCACAGCCCGAGAGGCGAGG
  ATGACAGCGTTAGCCCTGGAGGTGCACGCGTGCCTGGGTTGCGCGGCGCCGCGGGCAGCGGGGCTAGCGG
  CGGGCAGGCGGGCGGCGTGGAGGAGGAGGAAGAGCCCGCAGCCCCTAAACCGCGAAAGAAGCGCTCCCGG
  GCCGCCTTCTCGCACGCCCAGGTCTTCGAGCTGGAGCGCCGCTTTAACCATCAGCGCTACCTGTCCGGGC
  CGGAGCGCGCAGACCTGGCAGCTTCGCTGAAGCTCACAGAGACGCAAGTGAAGATCTGGTTTCAGAACCG
  TCGCTACAAGACCAAACGCCGGCAGATGGCCGCCGACCTGCTCGCCTCTGCACCCGCCGCCAAGAAAGTG
  GCCGTAAAGGTGCTGGTGCGTGACGACCAAAGACAGTATTTGCCCGGAGAGGTGCTGAGGCCACCTTCCC
  TTCTGCCACTGCAGCCCTCCTACTACTACCCTTACTACTGTCTCCCGGGCTGGGCGCTGTCCACGTGCGC
  GGCCGCTGCTGGTACCCAG
  
  CTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG
  ATAAGGTTTAA
  Protein Sequence

  >MR224227 representing NM_007524
  Red=Cloning site Green=Tags(s)
  
  MAVRGSGTLTPFSIQAILNKKEERGGLATPEGRPAPGGTEVAVTAAPAVCCWRIFGETEAGALGGAEDSL
  LASPARTRTAVGQSAESPGGWDSDSALSEENEGRRRCADVPGASGTGRARVTLGLDQPGCELHAAKDLEE
  EAPVRSDSEMSASVSGDHSPRGEDDSVSPGGARVPGLRGAAGSGASGGQAGGVEEEEEPAAPKPRKKRSR
  AAFSHAQVFELERRFNHQRYLSGPERADLAASLKLTETQVKIWFQNRRYKTKRRQMAADLLASAPAAKKV
  AVKVLVRDDQRQYLPGEVLRPPSLLPLQPSYYYPYYCLPGWALSTCAAAAGTQ
  
  LEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007524
• ORF Size: 999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007524.3, NP_031550.2
• RefSeq Size: 1437 bp
• RefSeq ORF: 1002 bp
• Locus ID: 12020
• UniProt ID: P97503
• Cytogenetics: 5 22.58 cM
• MW: 35.6 kDa
• Gene Summary: Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development; required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear; required for tympanic ring and gonium development and in the regulation of the width of the malleus.[UniProtKB/Swiss-Prot Function]