Smarca2 (NM_011416) Mouse Tagged ORF Clone

CAT#: MR224152

Smarca2 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (Smarca2), transcript variant 1

Product Images

Circular map for MR224152

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smarca2
• Synonyms: 2610209L14Rik; brahma; brm; SNF2alpha; Snf2l2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224152 representing NM_011416
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACACCCACAGACCCAGCAGCAATGCCCCATCCTGGGCCCTCCCCGGGGCCTGGACCCTCTCCTG
  GACCAATTCTGGGGCCTAGTCCAGGACCAGGACCATCCCCAGGTTCTGTGCACAGCATGATGGGTCCTAG
  TCCCGGACCTCCCAGCGTCTCACATCCTCTGTCAACGATGGGCTCTGCAGACTTCCCACAGGAAGGCATG
  CACCAATTACATAAGCCCATGGATGGGATACATGACAAAGGGATTGTAGAAGATGTCCACTGTGGATCCA
  TGAAGGGCACCAGCATGCGCCCCCCACACCCAGGAATGGGCCCTCCACAGAGCCCCATGGACCAGCACAG
  CCAAGGTTATATGTCACCACATCCGTCTCCTCTGGGAGCCCCGGAGCACGTCTCTAGCCCTATATCTGGA
  GGAGGCCCAACCCCACCCCAGATGCCACCGAGCCAGCCAGGGGCACTCATCCCAGGAGATCCGCAGGCCA
  TGAACCAGCCTAACAGAGGTCCCTCGCCTTTCAGTCCTGTGCAGCTGCATCAGCTTCGAGCTCAGATTTT
  AGCTTACAAAATGTTGGCCAGGGGCCAGCCTCTCCCTGAAACTCTGCAGCTGGCAGTCCAGGGAAAAAGG
  ACCTTGCCTGGCATGCAGCAGCAGCAGCAGCAACAACAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGC
  AGCAACAGCAGCAACAACAGCAGCCCCAGCAGCCTCAGCAGCAGGCTCAGGCACAGCCCCAGCAGCAGCA
  GCAACAGCAGCAGCAGCCAGCTCTTGTTAGCTATAATCGACCATCTGGCCCCGGGCAGGAGCTGCTACTG
  AGTGGCCAGAGCGCTCCGCAGAAGCTGTCAGCACCAGCACCAAGCGGCCGACCTTCACCGGCACCCCAGG
  CCGCCGTCCAGCCCACGGCCACAGCGGTGCCCGGGCCCTCCGTGCAGCAGCCCGCCCCAGGGCAGCCGTC
  TCCGGTCCTACAGCTGCAACAGAAGCAGAGCCGCATCAGCCCCATCCAGAAACCGCAAGGCCTGGACCCG
  GTGGAGATCCTGCAGGAACGAGAGTACAGACTTCAAGCTCGCATCGCTCATAGGATACAAGAACTGGAAA
  GTCTGCCTGGTTCCTTGCCACCAGATTTACGCACCAAAGCAACCGTGGAACTGAAAGCACTTCGCTTACT
  CAACTTCCAACGTCAGCTGAGACAGGAGGTGGTGGCCTGCATGCGGAGGGACACCACCCTGGAGACGGCC
  CTCAACTCCAAAGCATATAAGCGGAGCAAGCGCCAGACCCTGCGTGAGGCACGCATGACAGAGAAACTGG
  AGAAGCAGCAGAAGATAGAACAGGAGAGGAAACGCCGGCAGAAACACCAGGAATACCTGAACAGTATTTT
  GCAACATGCAAAAGATTTTAAGGAATATCACCGGTCTGTGGCCGGGAAGATCCAGAAGCTCTCCAAAGCA
  GTGGCGACTTGGCATGCTAACACAGAAAGGGAGCAGAAGAAGGAGACGGAGCGGATCGAGAAGGAGAGAA
  TGCGGAGGCTGATGGCCGAAGATGAAGAGGGCTACAGGAAGCTTATTGACCAAAAGAAAGACAGACGTCT
  CGCCTACCTATTGCAGCAGACCGATGAGTATGTCGCCAATCTGACCAACCTGGTGTGGGAGCACAAGCAG
  GCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAGGAAGAAGAAGGCTGAAGAGAATGCAGAGGGAG
  GGGAACCTGCCCTGGGACCAGATGGAGAGCCAATAGATGAAAGCAGCCAGATGAGTGACCTGCCTGTCAA
  AGTGACACACACAGAAACTGGCAAGGTCCTCTTTGGACCAGAAGCACCCAAAGCAAGTCAGCTGGATGCC
  TGGCTGGAGATGAATCCTGGTTACGAAGTTGCACCCAGATCTGACAGTGAAGAGAGTGAATCGGACTACG
  AGGAGGAGGATGAAGAAGAAGAGTCCAGTAGGCAGGAAACCGAGGAGAAGATACTGCTGGATCCCAACAG
  TGAAGAAGTTTCCGAAAAGGATGCCAAGCAGATCATTGAGACTGCGAAGCAGGACGTGGACGACGAATAC
  AGCATGCAGTACAGTGCCAGAGGCTCTCAGTCCTACTACACGGTGGCTCACGCTATCTCTGAGAGGGTGG
  AGAAGCAGTCTGCCCTCCTCATTAACGGCACCCTAAAGCATTACCAGCTCCAGGGCCTGGAATGGATGGT
  TTCCCTGTATAATAACAATCTGAACGGAATCTTAGCTGATGAAATGGGGCTAGGCAAGACCATCCAGACC
  ATTGCACTCATCACGTATCTGATGGAGCACAAAAGGCTCAATGGTCCCTACCTCATCATCGTCCCCCTCT
  CGACTCTGTCTAACTGGACATATGAATTTGACAAATGGGCTCCTTCTGTGGTGAAAATTTCTTACAAGGG
  TACCCCTGCCATGCGACGCTCCCTCGTTCCCCAGCTACGGAGTGGCAAATTCAATGTCCTCCTGACTACT
  TACGAGTACATTATAAAAGACAAGCACATTCTTGCAAAGATTCGGTGGAAGTACATGATCGTGGACGAAG
  GCCACCGGATGAAGAATCACCACTGCAAGCTAACCCAGGTCCTGAACACACACTATGTGGCCCCCAGGCG
  GATCCTTCTGACTGGGACCCCACTGCAGAATAAGCTTCCGGAACTCTGGGCCCTCCTCAACTTCCTCCTC
  CCTACAATCTTCAAGAGTTGCAGCACATTTGAGCAGTGGTTTAATGCTCCATTTGCCATGACCGGTGAAA
  GGGTGGACCTGAACGAAGAAGAAACGATTTTGATCATCAGGCGTCTACACAAGGTGCTGAGACCCTTTTT
  ACTGAGGAGGCTGAAGAAAGAGGTTGAGTCTCAGCTTCCGGAAAAGGTTGAGTATGTGATCAAGTGTGAC
  ATGTCAGCTCTGCAGAAGATTCTGTACCGTCACATGCAAGCCAAGGGGATCCTCCTCACGGACGGGTCTG
  AGAAAGATAAGAAGGGGAAAGGAGGTGCCAAGACACTTATGAACACCATCATGCAGCTGAGAAAAATATG
  CAACCACCCATATATGTTTCAGCACATTGAGGAATCCTTTGCTGAACACCTGGGCTATTCGAATGGGGTC
  ATCAATGGGGCTGAGCTGTATCGGGCCTCGGGAAAGTTTGAGCTGCTTGATCGTATTCTGCCCAAATTGA
  GAGCGACTAACCACCGCGTGCTGCTTTTCTGCCAGATGACGTCACTCATGACCATTATGGAGGATTACTT
  TGCTTTTCGGAACTTCCTGTACCTGCGCCTTGACGGCACCACCAAGTCTGAAGATCGTGCTGCTTTGCTA
  AAGAAATTCAATGAACCTGGGTCCCAGTATTTCATTTTCTTGCTGAGCACAAGAGCAGGGGGCCTGGGCT
  TAAATCTTCAGGCGGCAGACACGGTGGTCATATTTGACAGCGACTGGAATCCTCACCAGGATCTGCAGGC
  CCAAGACCGAGCTCACCGCATTGGCCAACAAAACGAGGTCCGGGTGCTGAGGCTTTGCACCGTCAACAGT
  GTGGAGGAAAAGATTCTCGCGGCTGCCAAGTACAAGCTGAACGTGGATCAGAAGGTTATCCAAGCAGGCA
  TGTTTGACCAGAAGTCATCCAGCCACGAGCGGAGGGCCTTCCTGCAGGCCATTCTGGAGCACGAGGAGGA
  GAATGAGGAAGAAGATGAGGTACCAGACGACGAGACCCTGAACCAGATGATTGCTCGCCGGGAGGAAGAA
  TTTGATCTTTTTATGCGCATGGACATGGACCGGCGGAGGGAGGATGCCCGGAACCCGAAGCGCAAACCCC
  GCTTGATGGAGGAAGATGAGCTGCCCTCCTGGATTATCAAGGATGACGCCGAAGTGGAAAGGCTCACCTG
  TGAAGAAGAGGAGGAGAAGATATTTGGGAGGGGCTCTCGCCAGCGCCGGGATGTGGACTACAGTGATGCC
  CTCACCGAGAAGCAATGGCTCAGGGCCATCGAAGACGGCAATTTGGAAGAAATGGAAGAGGAGGTACGGC
  TTAAGAAGAGAAAAAGACGAAGAAATGTGGATAAAGACCCCGTGAAGGAAGATGTGGAAAAAGCGAAGAA
  AAGAAGAGGCCGCCCTCCGGCTGAGAAGTTGTCACCAAATCCCCCAAAACTAACGAAGCAGATGAACGCC
  ATCATTGATACTGTGATAAACTACAAAGACAGTTCAGGGCGACAGCTCAGTGAAGTCTTCATTCAGTTAC
  CTTCCAGGAAAGACTTACCAGAATACTATGAATTAATTAGGAAGCCAGTGGATTTCAAAAAGATAAAGGA
  GCGAATCCGTAATCATAAGTATCGGAGCCTGGGAGACCTGGAGAAAGACGTCATGCTTCTCTGTCACAAC
  GCACAGACATTCAACTTGGAAGGATCCCAGATCTACGAAGACTCCATTGTCCTACAGTCAGTGTTTAAGA
  GTGCTCGGCAGAAAATTGCCAAAGAAGAAGAGAGTGAGGAAGAAAGCAATGAAGAAGAGGAAGAAGATGA
  TGAAGAGGAGTCGGAGTCAGAGGCGAAATCTGTGAAGGTGAAAATCAAGCTGAATAAAAAGGAAGAGAAA
  GGCCGGGACACAGGGAAGGGCAAGAAGCGGCCAAACCGAGGCAAAGCCAAACCCGTCGTGAGCGATTTTG
  ACAGTGACGAGGAACAGGAAGAGAACGAACAGTCAGAAGCAAGTGGAACTGATAACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224152 representing NM_011416
  Red=Cloning site Green=Tags(s)
  
  MSTPTDPAAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPLSTMGSADFPQEGM
  HQLHKPMDGIHDKGIVEDVHCGSMKGTSMRPPHPGMGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPISG
  GGPTPPQMPPSQPGALIPGDPQAMNQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKR
  TLPGMQQQQQQQQQQQQQQQQQQQQQQQQQQPQQPQQQAQAQPQQQQQQQQQPALVSYNRPSGPGQELLL
  SGQSAPQKLSAPAPSGRPSPAPQAAVQPTATAVPGPSVQQPAPGQPSPVLQLQQKQSRISPIQKPQGLDP
  VEILQEREYRLQARIAHRIQELESLPGSLPPDLRTKATVELKALRLLNFQRQLRQEVVACMRRDTTLETA
  LNSKAYKRSKRQTLREARMTEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSVAGKIQKLSKA
  VATWHANTEREQKKETERIEKERMRRLMAEDEEGYRKLIDQKKDRRLAYLLQQTDEYVANLTNLVWEHKQ
  AQAAKEKKKRRRRKKKAEENAEGGEPALGPDGEPIDESSQMSDLPVKVTHTETGKVLFGPEAPKASQLDA
  WLEMNPGYEVAPRSDSEESESDYEEEDEEEESSRQETEEKILLDPNSEEVSEKDAKQIIETAKQDVDDEY
  SMQYSARGSQSYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWMVSLYNNNLNGILADEMGLGKTIQT
  IALITYLMEHKRLNGPYLIIVPLSTLSNWTYEFDKWAPSVVKISYKGTPAMRRSLVPQLRSGKFNVLLTT
  YEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRILLTGTPLQNKLPELWALLNFLL
  PTIFKSCSTFEQWFNAPFAMTGERVDLNEEETILIIRRLHKVLRPFLLRRLKKEVESQLPEKVEYVIKCD
  MSALQKILYRHMQAKGILLTDGSEKDKKGKGGAKTLMNTIMQLRKICNHPYMFQHIEESFAEHLGYSNGV
  INGAELYRASGKFELLDRILPKLRATNHRVLLFCQMTSLMTIMEDYFAFRNFLYLRLDGTTKSEDRAALL
  KKFNEPGSQYFIFLLSTRAGGLGLNLQAADTVVIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNS
  VEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEENEEEDEVPDDETLNQMIARREEE
  FDLFMRMDMDRRREDARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEEEKIFGRGSRQRRDVDYSDA
  LTEKQWLRAIEDGNLEEMEEEVRLKKRKRRRNVDKDPVKEDVEKAKKRRGRPPAEKLSPNPPKLTKQMNA
  IIDTVINYKDSSGRQLSEVFIQLPSRKDLPEYYELIRKPVDFKKIKERIRNHKYRSLGDLEKDVMLLCHN
  AQTFNLEGSQIYEDSIVLQSVFKSARQKIAKEEESEEESNEEEEEDDEEESESEAKSVKVKIKLNKKEEK
  GRDTGKGKKRPNRGKAKPVVSDFDSDEEQEENEQSEASGTDNE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011416
• ORF Size: 4749 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011416.2, NP_035546.2
• RefSeq Size: 5831 bp
• RefSeq ORF: 4752 bp
• Locus ID: 67155
• UniProt ID: Q6DIC0
• Cytogenetics: 19 21.17 cM
• MW: 180.7 kDa
• Gene Summary: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.[UniProtKB/Swiss-Prot Function]