Ick (NM_019987) Mouse Tagged Lenti ORF Clone

CAT#: MR223892L3

Lenti ORF clone of Ick (Myc-DDK-tagged) - Mouse intestinal cell kinase (Ick), transcript variant 2

Product Images

Circular map for MR223892L3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ick
• Synonyms: 2210420N10Rik; AI848300; Mrk
• Vector: pLenti-C-Myc-DDK-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(MR223892).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019987
• ORF Size: 1890 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019987.2, NP_064371.2
• RefSeq Size: 6640 bp
• RefSeq ORF: 1890 bp
• Locus ID: 56542
• UniProt ID: Q9JKV2
• Cytogenetics: 9 E1
• Gene Summary: Has an essential role in ciliogenesis, particularly in neuronal and retinal progenitor cells (PubMed:24797473). Phosphorylates KIF3A (PubMed:24797473). Involved in the control of ciliary length (PubMed:24853502). Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (PubMed:24797473, PubMed:24853502). May play a role in cardiac development (By similarity). Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling -dependent manner and this regulation requires its kinase activity (PubMed:25243405).[UniProtKB/Swiss-Prot Function]