Ick (NM_019987) Mouse Tagged ORF Clone

CAT#: MG209586

Ick (tGFP-tagged) - Mouse intestinal cell kinase (Ick)

Product Images

Circular map for MG209586

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ick
• Synonyms: 2210420N10Rik; AI848300; Mrk
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209586 representing NM_019987
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAGATACACAACGATCAAGCAGCTTGGGGATGGGACCTACGGCTCTGTCCTGCTGGGGAGAAGCA
  TTGAGTCTGGAGAACTGATTGCCATTAAAAAAATGAAAAGAAAGTTTTACTCCTGGGAAGAATGCATGAA
  CCTTCGGGAGGTTAAGTCTTTAAAGAAGCTTAATCATGCCAATATCGTAAAGTTAAAAGAGGTTATCAGG
  GAAAACGACCATCTTTACTTTATCTTCGAGTACATGAAGGAAAACCTTTACCAGCTCATTAAAGAAAGAA
  ATAAGTTGTTTCCAGAGTCTGCTATAAGGAATATAATGTACCAGATATTGCAAGGACTGGCATTCATTCA
  CAAACACGGTTTCTTCCACCGGGACTTAAAACCCGAGAACCTCCTCTGCATGGGACCAGAACTTGTGAAA
  ATCGCCGACTTTGGATTGGCTCGAGAAATCCGATCAAGACCTCCGTACACAGACTATGTGTCTACTAGAT
  GGTACAGGGCTCCAGAGGTACTGCTGAGATCCACCAACTACAGCTCCCCCATTGACATCTGGGCGGTAGG
  CTGCATCATGGCGGAGGTGTACACGCTCCGGCCCCTTTTCCCTGGGGCCAGTGAAATTGACACAATTTTC
  AAAATTTGCCAAGTGTTGGGAACACCAAAGAAGACTGACTGGCCTGAAGGCTACCAGCTGTCAAGTGCTA
  TGAATTTCCTCTGGCCCCAGTGCATACCCAATAACTTAAAGACTCTAATCCCAAATGCTAGCAGCGAAGC
  CATTCAGCTCCTGAGAGACTTGCTTCAGTGGGATCCCAAGAAACGACCAACCGCTAGTCAGGCTCTTCGA
  TATCCTTACTTCCAGATCGGACACCCACTGGGCATCATCAGCAAGGACTCAGGAAAACCACAGAGAGAGG
  TCCAGGACAAGACAGGCCCTCCTCCTTACATCAAGCCAGCCCCTCCTGCCCAGGCCCCGGCCAAGGCATA
  TACGCTGATTTCTTCACGACCAAGCCAAGCCAGTCAGCCCCCTCAGCATTCTGTGCACCCCTACAAAGGC
  GATGTCTCTAGGACTGAGCAGCTGAGCCATGTTCAGGAGGGCAAGCCAAGCCCTCCATTCTTCCCATCCC
  TCCACAATAAGAATCTTCAGCCAAAAATCCTAGCCAGCCTGGAACAGAAAAATGGTGAAATCAAGCCAAA
  GAGCAGGAGAAGATGGGGTCTCATCTCCAGGTCGACAAAGGGTTCTGACGACTGGGCCGACTTGGATGAC
  TTAGACTTCAGTCCCTCTCTCACCAGGATAGATGTAAAAAACAAGAAGAGACAGAGTGATGACACCCTCT
  GCAGGTTTGAAAGTGTTCTGGACCTGAAGCCATCTGAGTCAGTGGGTACAGGAACCACTGTCTCCACGCA
  GGCTTCCTCGCAGAGGAGAGACACGCCGACCCTGCAGTCCTCAGCAAAGCAGCACTACTTGAAGCACTCT
  CGCTACTTGCCTGGAATAAATATAAGAAATGGCGTGCTCCCAAATCCAGGCAAGGACTTTCTTCCATCAA
  ATTCATGGTCCAGTTCCGGCTTGTCTGGAAAATCTTCAGGAACAGTATCAGTAGTGAGCAAAATAACTTC
  AGTTGGCTCAGGCTCTGCGAGCTCTAGTGGACTGACTGGAAGCTACATCCCTTCCTTTCTGAAAAAGGAA
  ATCGGTTCTGTCATGCAGAGGGTTCAGCTGGCGCCCCTCGCAGCCCCTCCCCCCGGCTACTCTTCCCTCA
  AGGCCGTGAGACCTCACCCGGGGAGGCCTTTCTTTCACACCCAGCCCAGGAGCACGCCCGGCTTGATCCC
  CCGACCTCCAGCTGCTCAGCCTGTGCACGGCAGGATAGACTGGTCTTCCAAGTACCCATCCCGGCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209586 representing NM_019987
  Red=Cloning site Green=Tags(s)
  
  MNRYTTIKQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANIVKLKEVIR
  ENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVK
  IADFGLAREIRSRPPYTDYVSTRWYRAPEVLLRSTNYSSPIDIWAVGCIMAEVYTLRPLFPGASEIDTIF
  KICQVLGTPKKTDWPEGYQLSSAMNFLWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTASQALR
  YPYFQIGHPLGIISKDSGKPQREVQDKTGPPPYIKPAPPAQAPAKAYTLISSRPSQASQPPQHSVHPYKG
  DVSRTEQLSHVQEGKPSPPFFPSLHNKNLQPKILASLEQKNGEIKPKSRRRWGLISRSTKGSDDWADLDD
  LDFSPSLTRIDVKNKKRQSDDTLCRFESVLDLKPSESVGTGTTVSTQASSQRRDTPTLQSSAKQHYLKHS
  RYLPGINIRNGVLPNPGKDFLPSNSWSSSGLSGKSSGTVSVVSKITSVGSGSASSSGLTGSYIPSFLKKE
  IGSVMQRVQLAPLAAPPPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRIDWSSKYPSRR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019987
• ORF Size: 1887 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019987.1
• RefSeq Size: 2326 bp
• RefSeq ORF: 1890 bp
• Locus ID: 56542
• UniProt ID: Q9JKV2
• Cytogenetics: 9 E1
• Gene Summary: Has an essential role in ciliogenesis, particularly in neuronal and retinal progenitor cells (PubMed:24797473). Phosphorylates KIF3A (PubMed:24797473). Involved in the control of ciliary length (PubMed:24853502). Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (PubMed:24797473, PubMed:24853502). May play a role in cardiac development (By similarity). Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling -dependent manner and this regulation requires its kinase activity (PubMed:25243405).[UniProtKB/Swiss-Prot Function]