Cdh20 (NM_011800) Mouse Tagged ORF Clone

CAT#: MR223808

Cdh20 (Myc-DDK-tagged) - Mouse cadherin 20 (Cdh20)

Product Images

Circular map for MR223808

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdh20
• Synonyms: Cdh7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223808 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACTACGGGTAGAATGAGCAATGCAAAGAGTTGGCTTGGACTTGGCACATCCTTGTACTTCTGGG
  CGCTGATGGACCTCACAGCCACTGTTCTCTCCAGCACTCCAATGCCAGAAGTTGAATTAGAAACACTCTT
  CTCTGGAAGGTCGCAGTCACATCAGCGAAGCAAGAGGAGCTGGGTTTGGAATCAGTTTTTTGTCCTGGAA
  GAGTACACTGGGACTGACCCTTTGTATGTCGGCAAGCTTCATTCAGACATGGACAGGGGAGATGGATCCA
  TCAAATACATCCTTTCAGGAGAAGGAGCAGGCATCGTGTTTACCATTGATGACACCACTGGAGACATCCA
  TGCCATTCAGAGGCTCGACCGAGAAGAAAGAGCCCAATATACTCTAAGGGCTCAAGCCCTAGACAGGAGA
  ACAGGCCGGCCAATGGAGCCAGAATCAGAGTTCATTATCAAAATCCAAGATATCAATGACAACGAGCCCA
  AGTTCCTGGATGGACCTTACATCGCCACTGTGCCAGAAATGTCACCAGTAGGTACATCTGTTATCCAAGT
  GACAGCCACAGATGCTGATGACCCAACCTATGGCAACAGTGCCCGGGTAGTGTACAGCATTCTCCAGGGC
  CAGCCATATTTTTCTGTGGACTCCAAAACAGGTGTAATCAGAACAGCCCTTATGAACATGGACAGAGAAG
  CCAAGGAGTACTATGAAGTGATTATCCAAGCCAAAGATATGGGAGGGCAACTTGGAGGATTGGCTGGGAC
  CACAACAGTCAACATCACTCTCTCAGATGTCAATGATAACCCACCCCGATTTCCACAGAAACATTATCAG
  ATGAGTGTGCTAGAATCTGCTCCAATTAGCTCTACTGTGGGCAGAGTGTTTGCCAAGGACCTGGATGAAG
  GAATCAATGCAGAGATGAAGTATACCATTGTGGATGGAGATGGTGCAGATGCTTTTGATATTAATACAGA
  CCAAAACTTCCAAGTTGGCATCATAACTGTGAAGAAGCCCCTGAGTTTTGAAAGCAAGAAAAGCTACACC
  CTGAAGGTGGAAGGATCCAATCCTCACCTAGAGATGCGTTTTCTGAACCTTGGCCCATTTCAGGACACAA
  CAACAGTGCACATCAGTGTGGAGGATGTGGACGAGCCCCCTGTGTTTGAGCCTGGCTTTTATTTTGTGGA
  GGTGCCAGAGGATGTGACAATTGGAACAACCATACAGATCATTTCTGCTAAGGACCCAGACGTAACCAAC
  AACTCAATCAGGTACTCCATTGACAGAGGCAGCGACCCGGGAAGATTTTTCTATGTGGACATTACAACAG
  GTGCTCTAATGACTGCAAGACCACTAGACAGAGAAGAGTTCTCCTGGCATAATATAACTGTCCTTGCTAT
  GGAAATGAACAATCCCTCCCAAGTGGGAAGTGTTGCTGTCACAATCAAAGTCCTCGATGTGAATGACAAC
  GCTCCAGAGTTTCCCAGATTCTATGAAGCTTTTATCTGTGAAAATGCCAAAGCCGGACAGCTGATCCAGA
  CAGTGAGTGCTGTGGATCAAGATGACCCTCACAATGGCCAACATTTCTACTACAGTTTGGCTCCTGAGGC
  TGCTAACAACCCCAACTTCACTGTCAGGGACAACCAAGATAATACTGCTCGGATTCTGACCCGGAGGTCC
  GGCTTCCGACAGCAGGAGCAGAGCGTCTTCTACCTGCCCATCCTCATCGCAGACAGCGGCCAGCCCGTCC
  TCAGCAGCACCGGCACGCTTACCATTCAAGTGTGCAGCTGCAATGACGATGGCCACGTCATGTCCTGCAG
  CCCAGAGGCCTATCTGCTCCCAGTCAGCTTGAGTCGGGGTGCCCTCATCGCCATCCTGGCCTGCATCTTT
  GTCCTCTTAGTGTTGGTGCTGCTCATCCTGTCCATGAGGCGACATCGCAAACAACCCTACATCATCGACG
  ACGATGAGAACATCCACGAGAACATTGTGCGCTACGACGACGAGGGGGGCGGCGAAGAGGACACAGAGGC
  CTTCGACATCGCAGCCATGTGGAACCCGCGCGAGGCGCAGGCAGGCGCCGCCCCCAAGACGCGCCAGGAC
  ATGCTCCCCGAGATCGAGAGCCTGTCCCGCTACGTGCCTCAGACCTGCGCGGTCAGCAGCACGGTCCACA
  GCTACGTGCTGGCCAAGCTCTACGAGGCCGACATGGACCTGTGGGCCCCGCCCTTCGACTCCCTGCAGAC
  CTACATGTTTGAAGGGGACGGCTCTGTGGCCGGCTCGCTCAGCTCCCTGCAGTCAGCCACCTCGGACTCA
  GAGCAGAGCTTTGATTTCCTCACGGACTGGGGACCCCGCTTCCGAAAACTGGCAGAACTCTACGGGGCGT
  CCGAGGGGCCCGCGCCCTTGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223808 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWTTGRMSNAKSWLGLGTSLYFWALMDLTATVLSSTPMPEVELETLFSGRSQSHQRSKRSWVWNQFFVLE
  EYTGTDPLYVGKLHSDMDRGDGSIKYILSGEGAGIVFTIDDTTGDIHAIQRLDREERAQYTLRAQALDRR
  TGRPMEPESEFIIKIQDINDNEPKFLDGPYIATVPEMSPVGTSVIQVTATDADDPTYGNSARVVYSILQG
  QPYFSVDSKTGVIRTALMNMDREAKEYYEVIIQAKDMGGQLGGLAGTTTVNITLSDVNDNPPRFPQKHYQ
  MSVLESAPISSTVGRVFAKDLDEGINAEMKYTIVDGDGADAFDINTDQNFQVGIITVKKPLSFESKKSYT
  LKVEGSNPHLEMRFLNLGPFQDTTTVHISVEDVDEPPVFEPGFYFVEVPEDVTIGTTIQIISAKDPDVTN
  NSIRYSIDRGSDPGRFFYVDITTGALMTARPLDREEFSWHNITVLAMEMNNPSQVGSVAVTIKVLDVNDN
  APEFPRFYEAFICENAKAGQLIQTVSAVDQDDPHNGQHFYYSLAPEAANNPNFTVRDNQDNTARILTRRS
  GFRQQEQSVFYLPILIADSGQPVLSSTGTLTIQVCSCNDDGHVMSCSPEAYLLPVSLSRGALIAILACIF
  VLLVLVLLILSMRRHRKQPYIIDDDENIHENIVRYDDEGGGEEDTEAFDIAAMWNPREAQAGAAPKTRQD
  MLPEIESLSRYVPQTCAVSSTVHSYVLAKLYEADMDLWAPPFDSLQTYMFEGDGSVAGSLSSLQSATSDS
  EQSFDFLTDWGPRFRKLAELYGASEGPAPLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011800
• ORF Size: 2406 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011800.1
• RefSeq Size: 3722 bp
• RefSeq ORF: 2406 bp
• Locus ID: 23836
• UniProt ID: Q9Z0M3
• Cytogenetics: 1 49.42 cM
• MW: 89 kDa
• Gene Summary: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (By similarity).[UniProtKB/Swiss-Prot Function]