Vrk1 (NM_011705) Mouse Tagged ORF Clone

CAT#: MR223700

Vrk1 (Myc-DDK-tagged) - Mouse vaccinia related kinase 1 (Vrk1), transcript variant 1

Product Images

Circular map for MR223700

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vrk1
• Synonyms: 51PK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223700 representing NM_011705
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGTGTAAAAGCAGCTCAGGCTGGAAGACCCGGACCTGCGAAGAGGCGCCTCGCAGAGCAGTTTG
  CCGCTGGAGAGGTCCTAACCGACATGTCTAGGAAGGAGTGGAAACTAGGATTGCCCATTGGCCAAGGTGG
  CTTTGGCTGCATCTATCTGGCGGACACAAATTCTTCCAAACCGGTTGGCAGTGACGCGCCCTGTGTTGTG
  AAAGTGGAACCCAGTGACAATGGACCTCTTTTCACGGAATTAAAGTTCTACCAGAGGGCTGCTAAACCAG
  AGCAAATTCAGAAATGGATTCGTACACATAAATTGAAGTACCTTGGTGTTCCTAAGTATTGGGGATCTGG
  TCTACATGATAAAAATGGAAAAAGTTACAGGTTTATGATAATGGACCGCTTTGGGAGTGACCTTCAGAAA
  ATATATGAAGCAAATGCCAAAAGGTTTTCTCGGAAAACTGTATTGCAGCTAAGCTTAAGAATTCTGGATA
  TCCTGGAGTACATCCATGAGCATGAGTACGTGCACGGGGACATCAAGGCCTCCAACCTGCTCCTGAGTCA
  CAAGAACCCTGACCAGGTATATTTGGTAGACTATGGCCTTGCTTATCGGTACTGCCCAGATGGAGTTCAT
  AAAGAGTACAAGGAAGATCCCAAAAGGTGCCATGACGGCACCCTGGAGTTCACCAGCATCGACGCTCACA
  AAGGCGTGGCCCCATCAAGACGTGGTGATTTGGAAATACTTGGTTATTGCATGATCCAGTGGCTCAGCGG
  CTGTCTTCCTTGGGAAGATAACTTGAAAGATCCTAACTACGTTAGGGATTCCAAAATTAGATACAGAGAC
  AACGTCGCAGCTTTGATGGAGAAATGCTTTCCTGAGAAAAATAAGCCAGGTGAGATCGCTAAGTACATGG
  AGTCTGTGAAACTACTGGAATACACCGAAAAACCTCTCTATCAAAACCTACGTGATATCCTTTTACAAGG
  ACTAAAAGCTATAGGAAGTAAAGACGACGGCAAACTGGATTTTAGTGCTGTGGAGAACGGAAGTGTGAAG
  ACAAGACCAGCCTCAAAGAAGCGGAAGAAAGAAGCAGAAGAAAGCGCGGTGTGCGCTGTGGAGGACATGG
  AGTGCTCAGACACACAGGTGCAAGAGGCCGCACAGACCCGAAGTGTGGAGTCCCAAGGAGCAATACATGG
  AAGCATGTCTCAGCCAGCGGCGGGCTGCAGCAGCTCTGACAGTTCCAGGAGGCAGCAGCATTTGGGATTG
  GAACAAGACATGCTAAGGCTAGACCGTAGGGGTTCACGAACCAGAAAGAAAGCCCAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223700 representing NM_011705
  Red=Cloning site Green=Tags(s)
  
  MPRVKAAQAGRPGPAKRRLAEQFAAGEVLTDMSRKEWKLGLPIGQGGFGCIYLADTNSSKPVGSDAPCVV
  KVEPSDNGPLFTELKFYQRAAKPEQIQKWIRTHKLKYLGVPKYWGSGLHDKNGKSYRFMIMDRFGSDLQK
  IYEANAKRFSRKTVLQLSLRILDILEYIHEHEYVHGDIKASNLLLSHKNPDQVYLVDYGLAYRYCPDGVH
  KEYKEDPKRCHDGTLEFTSIDAHKGVAPSRRGDLEILGYCMIQWLSGCLPWEDNLKDPNYVRDSKIRYRD
  NVAALMEKCFPEKNKPGEIAKYMESVKLLEYTEKPLYQNLRDILLQGLKAIGSKDDGKLDFSAVENGSVK
  TRPASKKRKKEAEESAVCAVEDMECSDTQVQEAAQTRSVESQGAIHGSMSQPAAGCSSSDSSRRQQHLGL
  EQDMLRLDRRGSRTRKKAQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011705
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011705.4
• RefSeq Size: 3796 bp
• RefSeq ORF: 1323 bp
• Locus ID: 22367
• UniProt ID: Q80X41
• Cytogenetics: 12 E- F1
• MW: 50.2 kDa
• Gene Summary: Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function]