Ccdc40 (NM_175430) Mouse Tagged ORF Clone

CAT#: MR223550

Ccdc40 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 40 (Ccdc40)

Product Images

Circular map for MR223550

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc40
• Synonyms: B930008I02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223550 representing NM_175430
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGATGCTGAGAAAGTCAGCACGGATGGGGAAGCCATCTCTGAAGGAGAAGTGGGGTCCAATGGAG
  AGACTCCCCCAGAAACAGAAGTGGAATTCATTGGAGAGACCGCTCCAGACACAGATGTGGAATTCATTGG
  AGAGACCTCCCCAGGAACAGACGTGGAACCCACTGGAGAGAGCATACAGGAAACAGAAGTTGAATCCATC
  GGAGAGGCTACCCCAGGAATGGACGTGGAACCCATTAAAAAGACCATGACAGAACTCAACGTTGAATCCA
  TCGGAGAAGAAACCTCAGAAACAGACGTGGACTCCATTAGGAAGGCCCTCCGAGGAATAGACTTGGAATC
  TATCACAGTTGCCTATCCGCCAAAAAAGGCTAAACACAGAAAGGTCAGACCCCAAGCGGAAGTTGAATCC
  ACTGGAAGAGCTGCCCCAGAAGGAGAATTAGAAGTTTCTGACCACGAGAAAGTAGAAGCCCTTCTCGATG
  AGTTGGATGAGCTCTCTGAGATAGTCAGCTCTCCCGAGGTCTCCTACTCTGACATCAGTCCCTTGGAGAT
  GGGTGAAGATGACACAAACGTCTCAGCCACATCAACCGACACATTCCAGCAAGGCATATATGAGCCCATA
  GAACCCATAGAGCCCACAGAGCCCCCAGAGCCTGCAGAGCCCCCAAAGCCTGCAGAGACCCCAGAGGACA
  GCACGGTGAGAGCCCCAGCGCATCCCTATCAAAGAGACTTCCCAATGGGGGCACGACACCGATTCAGACT
  GAGCATCATGGGAAGTCTGACTCCCTCGGACACGGACGATCTTCCCCTGGAAACGGACGAGCCGCCTCAG
  CAAGAGTCAGTGCAGTCAACTCCCAGAGCCCTTGAAGAAACCCGGATACAGTTCCTAGACCAGGTCCAGA
  GCCTCAGCCCGGAGGCCCTGCTCGATAGAGCAACTGAAGGGAGTGATGAAGCAGAGGAGGAAGGCTCCCA
  GCTGATCGTGCTGGACCCAGACCATCCTCTGATGATCAGATTCCAGGAGGCTCTGAAGGGCTACCTCAAC
  CGGCAGATGGACAAACTGAAGCTGGACGTCCAAGAGCTGGATGTGGCCACCAAGCAAACCCGAAGCCAGA
  GGCAGGAGCTGGGCGTGAACCTCTACGGGGTCCAGCAGCACCTGGCCCGCCTGCAGATGCAGCTGGAGAA
  GAGCCATGACCGGCACTCTCTGGTGGCCTGTGAGCGGAGAAGGAAGGAGGAGGAGCTGCAATGCGCACGC
  TCCGTCTACAACAAGACCTGCCAGACCGCCAATGAGGAACGAAAGAAGTTGGCCGCCCTGCAGACAGAGG
  TGGAGAGCCTGGCCCTGCACCTCTTCTACATGCAGAACATAGAGCAGGACGTGCGCGATGACATCCAAGT
  CATGAAGCAGGTGGTGAGGAAGACCGAGACGGAGAAGATGCACGCCGAGGTGGAGAAGAAAAAGCAGGAC
  CTGTTTGTGGACCAGCTCACCGAAAGGTCACACCAGCTAGAAGAAAACATCGCCCTGTTTGAGGCTCAAT
  ACTTGTCCCAGGCTGAGGACACCCGGGTCCTCAAGAAAGCAGTGACTGAGGCCATCACAGAGATTGACAC
  CATTGCCGTGGAGAAGAAGCGAATCCTACAGCAGTGGACCACCAGCCTAGTGGGCATGAAGCACCGTAAC
  GAGGCATACAAGACAGTGATGGATGCGCTCAGGGAGTGTCAGCATCAGGTGAAGTCCACCGACAGTGAGA
  TCGAAGTCTGCAAGAAGTCCATCATGCAGGAGGAAGAGAAGAATGAGAAGCTGGCCCGTCTCCTGAACCG
  CGCAGAGACAGAAGCCACACTGGTGCAGAAAATGACCGCCCAGTGCTTAAGCAAGCAAGAGGCTCTGCAG
  ACGGAGTTTAACACGTACCAGCTCGCCCTGCAGGACACGGAGGAGATGCTGAACAAGGGCTATGTGGAAC
  ACTCGGCAGTCTTGAGCGAGCTCCAGGCCACACGCCAGGCTTTCCACCAGGAACAGGAGCTACGGCAAAA
  GATGGACATGTCCATGGTGGACAAGCTGCAGGAACAGGGGACCTCCAGCAAGATGACCAAGTACTTTCAT
  CAGCTCCTTCGGAAGCTGCAAAAGGAAAACACCAACCTGGTGACACATCTTTCAAAAATTGACGGCGATA
  TCGCCCAGGCCACCCTGGACATCACCAACACCAACTGCAAAATAGACATGCATAAGAAGACGCTGGCAGA
  GATGGACAAGGAGGTAAAGAGGTTCAACGATCTCATCACCAACAGCGAGAGCGAGATCGCCCGGCGCACC
  ATCCTCATTGAGAGGAAGCAGAGCCTCATCAACTTCTTCAACAAGCAGCTGGAACAGATGGTGTCGGAGC
  TGGGGGGGGAAGAAGCTGGACCCCTGGAACTGGAGATCAAAAGGCTGTCGAAGCTGACAGAAGAGTACAA
  CACGGGAGTGGCTGAGGCGCAGATGACCTGGTTGCGCCTGCAGCAGGAACTGGTCCAGGTCACGCATGAG
  CGCGAGGAGCAGCTGGTGTCCGTGGACCAACTGAAGAAGGAGGTGCACATCATGGAACAAAAGAAGCTTC
  GCATAGAGAGCAAGATTGCACATGAGAAGAAGGAACAAAAGATAGTGAGCCGCCACATGAGGGACCTGGA
  CAACGACCTGAGCAAGCTCAACATGCTGTTGGACAAGAACCGATGCAGTTCGGAAGAGCTGGAGCAGAAC
  AATATAGCAACTGAGACGGAGTTTCTGCGCACGCTGAAGGACTCAGAAAGAGAGACCATTCAAATGCAGG
  AAAAGCTGATGGAACTCTCTGAGGAAAAGGCCACCCTCCTCAACAGCTTCATGGAGGCAGAGCACCAAAT
  CATGCTGTGGGAGAAGAAAATCCAACTGGCCAAGGAGATGCGCAGCTCGGTGGATTCTGAGACCGGGCAG
  ACAGAGATCCGTGCTATGAAGGCCGAGATCCACAGGATGAAGGTTAGGCACGGGCAGCTACTGAAGCAGC
  AGGAGAAGATGATCCGTGACATGGAGTTGGCTGTGGCCCGCAGGGAGACCATCGTCGTCCAGGCTGAAGG
  GCAGAGCAAGATAGACAAGAAAGTAATCACCAAGACAGAGTTCCACTACCAGCAGCGCGAGCTGCAGAAG
  AAAGTCAGGGAGATGCACAAGGCCACAGATGACTGCACCAACACCATCTCAGAACTGGAAGAAACTCAAA
  AGTTCCTAAGCAGCTCCCTCCAGGAGAAGCAGCAACTGCTGTCAGAAATGCAGGCTACCACAGATGTTCT
  TGAAGAGGAGATCAACCAGCTAACAGCTCTCAAACGGCAGAACCTTCTGGAGATCGTGACCCTGCAGACC
  CGTGGCAAGCACCTGCAGGCCGCCATTGAGGGGAAATATGTGTTCCTGCACCGCAACAGCAGGTCCCAGC
  TGATGGAGCGTAAGCGCCTGAGCGTCCGGCTGTCCCAGCTCAACAAGGTCCTCTCCAGCGTGCAGGAAGA
  CTACCCCCAGTACCAGGAGGTGCTGCAGAGCATTCAACAGAAGATCGCCACCAAGCTGGAGACCCCAGAG
  CCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223550 representing NM_175430
  Red=Cloning site Green=Tags(s)
  
  MMDAEKVSTDGEAISEGEVGSNGETPPETEVEFIGETAPDTDVEFIGETSPGTDVEPTGESIQETEVESI
  GEATPGMDVEPIKKTMTELNVESIGEETSETDVDSIRKALRGIDLESITVAYPPKKAKHRKVRPQAEVES
  TGRAAPEGELEVSDHEKVEALLDELDELSEIVSSPEVSYSDISPLEMGEDDTNVSATSTDTFQQGIYEPI
  EPIEPTEPPEPAEPPKPAETPEDSTVRAPAHPYQRDFPMGARHRFRLSIMGSLTPSDTDDLPLETDEPPQ
  QESVQSTPRALEETRIQFLDQVQSLSPEALLDRATEGSDEAEEEGSQLIVLDPDHPLMIRFQEALKGYLN
  RQMDKLKLDVQELDVATKQTRSQRQELGVNLYGVQQHLARLQMQLEKSHDRHSLVACERRRKEEELQCAR
  SVYNKTCQTANEERKKLAALQTEVESLALHLFYMQNIEQDVRDDIQVMKQVVRKTETEKMHAEVEKKKQD
  LFVDQLTERSHQLEENIALFEAQYLSQAEDTRVLKKAVTEAITEIDTIAVEKKRILQQWTTSLVGMKHRN
  EAYKTVMDALRECQHQVKSTDSEIEVCKKSIMQEEEKNEKLARLLNRAETEATLVQKMTAQCLSKQEALQ
  TEFNTYQLALQDTEEMLNKGYVEHSAVLSELQATRQAFHQEQELRQKMDMSMVDKLQEQGTSSKMTKYFH
  QLLRKLQKENTNLVTHLSKIDGDIAQATLDITNTNCKIDMHKKTLAEMDKEVKRFNDLITNSESEIARRT
  ILIERKQSLINFFNKQLEQMVSELGGEEAGPLELEIKRLSKLTEEYNTGVAEAQMTWLRLQQELVQVTHE
  REEQLVSVDQLKKEVHIMEQKKLRIESKIAHEKKEQKIVSRHMRDLDNDLSKLNMLLDKNRCSSEELEQN
  NIATETEFLRTLKDSERETIQMQEKLMELSEEKATLLNSFMEAEHQIMLWEKKIQLAKEMRSSVDSETGQ
  TEIRAMKAEIHRMKVRHGQLLKQQEKMIRDMELAVARRETIVVQAEGQSKIDKKVITKTEFHYQQRELQK
  KVREMHKATDDCTNTISELEETQKFLSSSLQEKQQLLSEMQATTDVLEEEINQLTALKRQNLLEIVTLQT
  RGKHLQAAIEGKYVFLHRNSRSQLMERKRLSVRLSQLNKVLSSVQEDYPQYQEVLQSIQQKIATKLETPE
  PS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175430
• ORF Size: 3576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175430.4, NP_780639.1
• RefSeq Size: 4979 bp
• RefSeq ORF: 3579 bp
• Locus ID: 207607
• UniProt ID: Q8BI79
• Cytogenetics: 11 E2
• MW: 137.2 kDa
• Gene Summary: Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Probably acts together with CCDC39 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella. Not required for outer dynein arm complexes assembly. Required for axonemal recruitment of CCDC39.[UniProtKB/Swiss-Prot Function]