Egflam (NM_178748) Mouse Tagged ORF Clone

CAT#: MR223436

Egflam (Myc-DDK-tagged) - Mouse EGF-like, fibronectin type III and laminin G domains (Egflam)

Product Images

Circular map for MR223436

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Egflam
• Synonyms: 5930412K08; AU040377
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223436 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTAATCTCAACCTTCTCGCTCCATTTCTTGCTCCTGGCCTGCAGCCTCCCACCCGGCGCTGTGT
  CACTCCGAACGGCTCTGCGAAAATCAGGCAAGGTGGGACCTCCTCTTGACATCAAGCTGGGAACCTTGAA
  CTGTACAGCTTTCAGCATCCAGTGGAAAACACCAAAGCGTTCTGGTAGCTCCATCATTGGCTACACAGTG
  TTTTACTCTGAGGTTGGTTCAGATAAGTCCCTGCGGGAGCGGTCACACAACGTCCCGGTTGGCCAGGATA
  CCCTAATCACTGAGGAAGTGATTGGAGATTTGAAGCCAGGCACTGAATATCAAGTAAGCGTAGCAGCCTA
  CAGTCAGACTGGCAAGGGGAGGCTTAGCTTCCCTCGACATGTGACCACTTTGTCTCAAGATTCCTGCCTG
  CCCCCTGCAGCACCCCAACAGCCACATGTCCTTGTGGTTTCTGATTCTGAAGTGGCCCTTTCTTGGAGAC
  CTGGAGAGAATGAAGGAAGTGCCCCAATTCAGTCCTACTCTGTGGAATTCATTAGACCAGATTTTGACAA
  GAGCTGGACCATAATCCAAGAGCGCCTGCAGATGGACTCCATGGTTATCAAAGGCCTTGATCCAGATACC
  AACTACCAGTTTGCTGTGAAAGCCATGAATGCTCACGGCTTCAGTCCGCGCAGCTGGCCCAGCAACACCG
  TCCGAACCCTGGGTCCCGGGGAGGCTGGAAGTGGTCATTATGGACCTGGGTATATCACCAATCCCGGTGT
  GAGTGAAGATGATGATGGATCTGAAGACGAACTGGACCTGGATGTATCCTTTGAGGAGGTTAAACCACTT
  CCTGCTACCAAAGTTGGGAATAAGAAGTTCTCAGTGGAGTCCAAGAAGACTTCTGTCTCCAACTCAGTGA
  TGGGCTCCAGACTCGCCCAACCTACCTCAGCCTCTCTCCACGAAACTACAGTGGCCATCCCACCCACACC
  AGCCCAACGGAAGGGGAAGAACAGTGTGGCCATGATGTCAAGGCTCTTTGACATGTCCTGTGATGAAACT
  CTCTGTTCAGCTGACAGCTTCTGTGTCAATGACTACGCCTGGGGGGGCTCACGATGCCATTGCAACCTGG
  GCAAAGGAGGCGAGGCCTGCTCAGAAGATATCTTTATTCAGTATCCTCAGTTCTTTGGCCACTCCTACGT
  AACCTTTGAGCCTCTGAAGAACTCTTATCAGGCATTTCAGGTTACTCTGGAGTTCAGGGCAGAGGCGGAG
  GATGGCTTGCTGCTCTACTGCGGAGAGAGTGAGCACGGAAGAGGAGACTTCATGTCCCTGGCTCTCATCC
  GGCGGTCCCTCCACTTCAGGTTTAATTGTGGAACTGGGATTGCCATCATCATAAGTGAGACCAAAATCAA
  ACTTGGGGCCTGGCATACGGTGACACTGTACAGAGATGGCCTGAATGGGATGCTGCAGTTGAATAATGGC
  ACCCCAGTGACAGGCCAGTCCCAGGGTCAGTACAGTAAAATTACCTTCCGGACACCTCTGTACCTCGGGG
  GCGCTCCCAGTGCTTACTGGCTGGTCAGAGCAACAGGGACAAACCGTGGCTTCCAAGGCTGTGTGCAGTC
  ACTTTCTGTTAATGGGAAGAAGATTGACATGAGGCCCTGGCCATTAGGAAAAGCCCTCAATGGAGCTGAT
  GTTGGGGAGTGCAGCAGTGGGATCTGTGACGAAGCCTCCTGCATCCACGGGGGCACCTGCGCAGCCATCA
  AGGCCGACTCCTACATCTGCCTCTGTCCCCTTGGGTTCAGAGGTCGACACTGTGAAGATGCTTTTGCCCT
  GACCATCCCTCAGTTCAGAGAGTCGCTGAGGTCCTATGCTGCCACACCCTGGCCCCTGGAGCCTCAGCAT
  TATCTTTCTTTCACCGAGTTCGAGATCACCTTTCGGCCAGACTCGGGGGATGGTGTCCTTTTGTACAGCT
  ACGACACGGGCAGCAAGGACTTCCTCTCCATCAATATGGCTGCGGGCCACGTGGAATTCCGCTTCGACTG
  TGGTTCTGGGACGGGAGTTCTCAGGAGTGAGGCTCCCCTCACCCTAGGCCAATGGCATGACCTACGTGTG
  TCTCGCACAGCGAAGAATGGGATCTTACAGGTGGACAAGCAGAAGGTGGTAGAGGGAATGGCAGAGGGAG
  GCTTCACCCAGATTAAGTGTAATACGGACATTTTTATTGGTGGAGTCCCAAATTACGATGATGTCAAGAA
  GAACTCGGGGATCCTCCATCCATTTAGTGGGAGCATTCAGAAGATCATCCTCAACGACCGGACCATCCAT
  GTGAAGCATGACTTTACATCGGGCGTGAATGTGGAGAACGCAGCCCACCCCTGTGTAGGGGCCCCCTGTG
  CCCATGGGGGCAGCTGTCGGCCCAGGAAGGAGGGTTATGAGTGCGACTGTCCCTTGGGCTTCGAAGGCCT
  GAACTGCCAGAAAGCTATTATAGAAGCCATCGAGATCCCACAGTTCATCGGCCGCAGCTACCTGACGTAT
  GACAACCCAAATATCCTCAAGAGGGTGTCTGGATCGAGGTCAAATGCGTTCATGAGGTTTAAGACAACCG
  CCAAGGATGGCCTGTTGCTGTGGAGGGGGGACAGCCCCATGAGACCTAACAGTGACTTCATTTCCCTGGG
  TCTTCGGGATGGAGCCCTGATATTCAGCTACAACCTGGGCAGTGGCGTGGCTTCCATCATGGTGAACGGT
  TCCTTCAGCGATGGCCGGTGGCACCGAGTCAAGGCTGTCAGGGATGGCCAGTCCGGGAAGATTACAGTGG
  ATGACTACGGAGCCAGAACAGGCAAGTCACCGGGCTTGATGCGGCAGCTTAACATCAACGGTGCTCTGTA
  CGTGGGTGGAATGAAGGAAATCGCTCTGCACACGAACAGACAATACCTGCGAGGCCTTGTGGGCTGCATC
  TCCCATTTCACACTGTCCACCGATTACCACATTTCCCTCGTGGAAGACGCCGTGGATGGGAAGAACATCA
  ACACTTGTGGGGCTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223436 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLISTFSLHFLLLACSLPPGAVSLRTALRKSGKVGPPLDIKLGTLNCTAFSIQWKTPKRSGSSIIGYTV
  FYSEVGSDKSLRERSHNVPVGQDTLITEEVIGDLKPGTEYQVSVAAYSQTGKGRLSFPRHVTTLSQDSCL
  PPAAPQQPHVLVVSDSEVALSWRPGENEGSAPIQSYSVEFIRPDFDKSWTIIQERLQMDSMVIKGLDPDT
  NYQFAVKAMNAHGFSPRSWPSNTVRTLGPGEAGSGHYGPGYITNPGVSEDDDGSEDELDLDVSFEEVKPL
  PATKVGNKKFSVESKKTSVSNSVMGSRLAQPTSASLHETTVAIPPTPAQRKGKNSVAMMSRLFDMSCDET
  LCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQVTLEFRAEAE
  DGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNG
  TPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMRPWPLGKALNGAD
  VGECSSGICDEASCIHGGTCAAIKADSYICLCPLGFRGRHCEDAFALTIPQFRESLRSYAATPWPLEPQH
  YLSFTEFEITFRPDSGDGVLLYSYDTGSKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRV
  SRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIH
  VKHDFTSGVNVENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKAIIEAIEIPQFIGRSYLTY
  DNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLGSGVASIMVNG
  SFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGLMRQLNINGALYVGGMKEIALHTNRQYLRGLVGCI
  SHFTLSTDYHISLVEDAVDGKNINTCGAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178748
• ORF Size: 3030 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178748.1, NM_178748.2, NM_178748.3, NM_178748.4, NM_178748.5, NM_178748.6, NP_848863.1
• RefSeq Size: 4864 bp
• RefSeq ORF: 3030 bp
• Locus ID: 268780
• UniProt ID: Q4VBE4
• Cytogenetics: 15 A1
• MW: 109.9 kDa
• Gene Summary: Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.[UniProtKB/Swiss-Prot Function]