Kdm5a (NM_145997) Mouse Tagged ORF Clone

CAT#: MR223212

Kdm5a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 5A (Kdm5a)

Product Images

Circular map for MR223212

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kdm5a
• Synonyms: AA409370; C76986; Jarid1a; Rbbp2; RBP2
• Vector: pCMV6-AC-Myc-DDK
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223212 representing NM_145997.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGTCCGTGGGCCCGGGGGGCTACGCGGCGGAGTTCGTGCCACCGCCCGAGTGCCCGGTCTTTGAG
  CCCAGTTGGGAGGAGTTCACAGACCCGCTGAGTTTTATCGGGCGCATCCGGCCCTTTGCGGAGAAAACG
  GGCATCTGCAAAATCCGGCCACCCAAGGATTGGCAACCTCCATTTGCCTGTGAAGTAAAAACCTTCCGT
  TTCACTCCTAGAGTCCAGCGCCTGAATGAACTTGAGGCAATGACTAGAGTAAGATTGGACTTCTTGGAT
  CAACTGGCAAAATTTTGGGAACTTCAAGGATCTACATTGAAGATCCCTGTGGTGGAGAGAAAAATCCTG
  GATTTGTATGCTTTAAGCAAGATTGTTGCCAGCAAAGGAGGTTTTGAAATAGTCACCAAAGAGAAGAAA
  TGGTCTAAAGTGGGTAGCCGCTTGGGATATCTGCCAGGAAAAGGAACTGGGTCTCTTTTGAAGTCACAC
  TATGAAAGAATTCTCTACCCATATGAACTTTTCCAATCTGGTGTCAGCCTTATGGGTGTACAAATGCCT
  GATTTAGATCTTAAAGAAAAAGTTGAGGCTGAGGTTCTCAGCACTGACATCCAGCCTTCCCCAGAGCGG
  GGAACAAGAATGAATATTCCACCAAAGAGAACAAGACGAGTTAAGTCTCAGTCAGACTCTGGAGAAGTA
  AATAGAAACACAGAACTGAAGAAACTTCAGATTTTTGGGGCTGGACCCAAAGTTGTGGGCCTGGCAGTA
  GGAGCAAAAGATAAAGAAGATGAAGTCACCCGAAGAAGAAAAGTTACCAACAGGTCAGATGCATTTAAC
  ATGCAAATGAGACAACGGAAAGGAACTCTCTCTGTTAACTTTGTTGATCTCTATGTTTGCATGTTTTGT
  GGTCGAGGAAATAATGAGGATAAACTGCTTTTGTGTGATGGGTGCGATGACAGCTATCATACATTTTGT
  CTACTTCCACCACTACCTGATGTCCCCAAAGGAGACTGGAGGTGTCCTAAATGTGTTGCTGAGGAATGT
  AACAAACCTCGAGAAGCCTTTGGATTTGAGCAAGCTGTAAGAGAATATACACTTCAAAGCTTTGGAGAG
  ATGGCAGATAATTTTAAATCTGATTATTTCAATATGCCAGTCCATATGGTTCCTACAGAGCTAGTAGAA
  AAAGAATTCTGGCGGCTAGTAAGTAGCATTGAAGAAGATGTTATTGTAGAGTATGGAGCTGATATCTCT
  TCAAAAGATTTTGGAAGTGGATTTCCTAAAAAGGATGGTCAAAGAAAGATGTTGCCAGAAGAAGAGGAA
  TATGCACTTTCTGGTTGGAATTTGAATAACATGCCTGTTCTGGAGCAGTCCGTTCTTGCACATATTAAT
  GTGGACATTTCTGGTATGAAGGTGCCATGGCTCTATGTGGGAATGTGCTTTTCTTCTTTTTGCTGGCAT
  ATTGAAGATCACTGGAGTTATTCCATTAACTACTTGCATTGGGGCGAGCCAAAGACATGGTATGGTGTA
  CCATCTCATGCTGCAGAACAACTGGAGGAGGTGATGAGGGAGCTGGCCCCTGAGTTATTTGAATCCCAG
  CCTGATCTCCTACATCAGTTAGTAACCATCATGAACCCAAATGTGCTCATGGAGCATGGTGTGCCTGTG
  TACAGGACTAATCAGTGTGCTGGAGAGTTTGTGGTGACGTTTCCTCGTGCCTATCACTCTGGATTTAAC
  CAGGGATACAACTTTGCCGAAGCGGTGAACTTCTGTACTGCTGACTGGTTGCCCATTGGACGTCAGTGT
  GTAAATCACTATCGACGGCTCAGGCGCCATTGTGTCTTTTCACATGAGGAACTCATTTTCAAGATGGCA
  GCAGATCCAGAATGCTTAGATGTGGGGCTGGCTGCCATGGTATGCAAAGAGTTGACTCTCATGACTGAA
  GAAGAAACACGATTGAGAGAATCAGTTGTTCAAATGGGTGTCGTGATGTCAGAAGAAGAAGTCTTTGAA
  CTTGTTCCTGATGATGAGCGGCAGTGTTCAGCATGCAGAACTACATGTTTTCTCTCAGCTCTCACATGC
  TCCTGTAATCCGGAGCGACTTGTATGTCTGTATCATCCAACTGATTTGTGCTCTTGCCCCATGCAGAAC
  AAATGTCTTAGGTACCGCTATCCACTAGAAGACCTTCCATCTCTGCTTTATGGTGTCAAAGTGAGAGCA
  CAATCCTATGACACTTGGGTCAATCGTGTTACAGAAGCATTGTCAGCTAGCTTCAACCATAAGAAAGAT
  TTGATTGAATTGAGAGTAATGCTGGAAGATGCTGAGGATAGGAAATACCCAGAGAATGATCTCTTTCGA
  AAGCTCAGGGACGCGGTGAAAGAAGCTGAGACCTGTGGTTCTGTGGCTCAGCTGCTTCTGAGCAAAAAG
  CAAAAACACAGGCAGAGCTCAGATAGTGGGAAGACTCGGACCAAACTGACAGTAGAAGAATTGAAGGCC
  TTTGTCCAACAACTTGTTAGTCTTCCGTGTGTCATCAGCCAAACTCGACAAGTAAAGAATCTGCTGGAT
  GATGTAGAAGAATTTCATGAACGTGCCCAGGAGGCCATGATGGATGAAACCCCAGATTCTTCCAAACTC
  CAAATGTTAATAGACATGGGCTCTAGTCTCTATGTGGAGCTACCTGAATTGCCCCGACTAAAGCAAGAG
  CTCCAGCAGGCTCGATGGCTGGATGAAGTAAGGCTAACTCTATCAGATCCACAACAAGTCACTTTGGAT
  GTCATGAAGAAATTGATTGACTCTGGCGTAGGCTTGGCACCTCACCATGCTGTAGAAAAAGCAATGGCC
  GAACTACAGGAACTCCTTACAGTCTCTGAACGATGGGAAGAGAAGGCTAAGGTCTGCCTGCAAGCAAGA
  CCTCGGCACAGTATGGCAAATTTAGAAAATATTGTGAATGAAGCCAAGAACATTCCAGCCTTTCTACCC
  AATGTGTTGTCCTTAAAAGAAGCCTTACAAAAGGCTCGTGAATGGACAGCTAAAGTGGAAGCTATTCAG
  AGTGGCAACAACTATGCTTACTTGGAACAGCTTGAGAGCCTATCTGCTAAAGGACGCCCTATCCCTGTG
  CGTCTTGATGCCTTGCCTCAAGTGGAGTCACAGGTAGCAGCAGCACGGGCCTGGAGAGAACGTACTGGG
  CGGACCTTCCTTAAAAAGAATTCCAGCCATACATTGTTACAGGTGCTAAGTCCACGAACTGACATTGGT
  GTATATGGGAGTGGTAAAAACAGGAGGAAAAAGGTAAAAGAAATAATAGAAAAAGAAAAGGAGAAGGAC
  CTCGACCTTGAGCCTTTGAGTGACTTAGAGGAAGGACTGGAGGAGTCAAGAGATACAGCCATGGTGGTG
  GCAGTTTTCAAAGAACGAGAGCAAAAGGAGATTGAAGCCATGCATTCCCTCAGAGCAGCCAACCTAGCC
  AAGATGACAATTGTGGAGCGCATAGAAGAAGTGAAATTTTGTATTTGCCGCAAGACAGCCAGTGGGTTT
  ATGCTACAGTGTGAGCTCTGCAAAGACTGGTTCCATAACAGCTGTGTTCCCCTTCCTAAATCAAGTTCC
  CAGAAAAAGGGATCCAGCTGGCAGGCTAAAGATGTAAAATTCCTTTGCCCTCTTTGCATGAGATCTCGG
  AGGCCAAGGCTAGAGACTATTCTGTCACTCCTGGTATCCCTTCAGAAGTTGCCTGTACGGTTGCCGGAA
  GGAGAAGCACTGCAGTGTTTGACAGAACGTGCCATGAGTTGGCAAGATAAAGCACGCCAAGCTTTAGCC
  ACAGATGAACTGTCCTCTGCCCTGGCCAAGCTCTCTGTGCTAAGCCAGCGTATGGTGGAACAAGCAGCT
  AGAGAAAAAACTGAAAAGATTATCAGTGCAGAACTCCAAAAAGCAGCTGCCAATCCAGATTTACAGGGA
  CACTTACCTAGTTTCCAGCAGTCTGCTTTTAACCGGGTGGTGAGCAGTGTATCATCTTCCCCCCACCAA
  ACAATGGACTATGATGATGAAGAAACAGATTCTGATGAAGATATTCGGGAGACATACGGCTATGACATG
  AAGGATACAGCCAGCGTGAAGTCCTCTAGTAGTCTGGAACCCAATCTCTTTTGTGATGAAGAGATTCCC
  ATCAAGTCTGAGGAAGTGGTGACTCACATGTGGACGGCACCGTCATTCTGTGCAGAGCATGCATATTCT
  TCTGCTTCAAAGAGTTGTTCTCAAGGTTCTAGCACTCCAAGAAAGCAGCCTCGGAAGAGCCCTTTGGTT
  CCCAGAAGTTTGGAGCCTCCAGTATTGGAATTGTCTCCTGGAGCAAAAGCCCAGTTGGAAGAACTCATG
  ATGGTTGGAGACCTCCTAGAAGTGTCTCTGGATGAGACACAACACATTTGGCGGATTTTGCAGGCCACA
  CATCCACCCTCAGAGGACAGATTCTTACATATCATGGAGGATGATAGCATAGAAGAGAAACCATTAAAA
  ATGAAAGGAAAGGACTCTTCAGAGAAGAAACGGAAACGGAAGCTAGAAAAGGTAGAACAGCTTTTTGGA
  GAAGGAAAACAGAAGACCAAGGAGCTAAAGAAAATAGATAAACCTAAAAAGAAGAAATTAAAATTAAAT
  GTGGACAAATCAAAAGAGCTGAATAAATTGGCCAAGAAGTTAGCTAAAGAAGAAGAGCGAAAGAAAAAG
  AAAGAAAAGGCTGCTGCCGCCAAAGTGGAGCTTGTGAAAGAGAGCACGGAGAAGAAACGGGAGAGGAAG
  GTGTTGGATATCCCCTCAAAGTATGACTGGTCGGGGGCAGAGGAGTCTGATGATGAGAATGCTGTGTGT
  GCAGCCCAAAACTGCCAAAGGCCGTGTAAGGACAAGGTAGACTGGGTACAGTGTGATGGTGGCTGTGAT
  GAGTGGTTTCATCAAGTTTGTGTTGGTGTATCCGCAGAAATGGCTGAAAATGAAGATTACATCTGTATA
  AACTGTGCAAAGAAGCAGGGGCCAGATAGCCCAGGCCAAGCACCACCTCCTCCCTTCCTAATGAGCTAC
  AAACTACCAATGGAGGATCTTAAAGAGACCAGT
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by MR223212
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MASVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPFAEKTGICKIRPPKDWQPPFACEVKTFR
  FTPRVQRLNELEAMTRVRLDFLDQLAKFWELQGSTLKIPVVERKILDLYALSKIVASKGGFEIVTKEKK
  WSKVGSRLGYLPGKGTGSLLKSHYERILYPYELFQSGVSLMGVQMPDLDLKEKVEAEVLSTDIQPSPER
  GTRMNIPPKRTRRVKSQSDSGEVNRNTELKKLQIFGAGPKVVGLAVGAKDKEDEVTRRRKVTNRSDAFN
  MQMRQRKGTLSVNFVDLYVCMFCGRGNNEDKLLLCDGCDDSYHTFCLLPPLPDVPKGDWRCPKCVAEEC
  NKPREAFGFEQAVREYTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVIVEYGADIS
  SKDFGSGFPKKDGQRKMLPEEEEYALSGWNLNNMPVLEQSVLAHINVDISGMKVPWLYVGMCFSSFCWH
  IEDHWSYSINYLHWGEPKTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIMNPNVLMEHGVPV
  YRTNQCAGEFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVNHYRRLRRHCVFSHEELIFKMA
  ADPECLDVGLAAMVCKELTLMTEEETRLRESVVQMGVVMSEEEVFELVPDDERQCSACRTTCFLSALTC
  SCNPERLVCLYHPTDLCSCPMQNKCLRYRYPLEDLPSLLYGVKVRAQSYDTWVNRVTEALSASFNHKKD
  LIELRVMLEDAEDRKYPENDLFRKLRDAVKEAETCGSVAQLLLSKKQKHRQSSDSGKTRTKLTVEELKA
  FVQQLVSLPCVISQTRQVKNLLDDVEEFHERAQEAMMDETPDSSKLQMLIDMGSSLYVELPELPRLKQE
  LQQARWLDEVRLTLSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLTVSERWEEKAKVCLQAR
  PRHSMANLENIVNEAKNIPAFLPNVLSLKEALQKAREWTAKVEAIQSGNNYAYLEQLESLSAKGRPIPV
  RLDALPQVESQVAAARAWRERTGRTFLKKNSSHTLLQVLSPRTDIGVYGSGKNRRKKVKEIIEKEKEKD
  LDLEPLSDLEEGLEESRDTAMVVAVFKEREQKEIEAMHSLRAANLAKMTIVERIEEVKFCICRKTASGF
  MLQCELCKDWFHNSCVPLPKSSSQKKGSSWQAKDVKFLCPLCMRSRRPRLETILSLLVSLQKLPVRLPE
  GEALQCLTERAMSWQDKARQALATDELSSALAKLSVLSQRMVEQAAREKTEKIISAELQKAAANPDLQG
  HLPSFQQSAFNRVVSSVSSSPHQTMDYDDEETDSDEDIRETYGYDMKDTASVKSSSSLEPNLFCDEEIP
  IKSEEVVTHMWTAPSFCAEHAYSSASKSCSQGSSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELM
  MVGDLLEVSLDETQHIWRILQATHPPSEDRFLHIMEDDSIEEKPLKMKGKDSSEKKRKRKLEKVEQLFG
  EGKQKTKELKKIDKPKKKKLKLNVDKSKELNKLAKKLAKEEERKKKKEKAAAAKVELVKESTEKKRERK
  VLDIPSKYDWSGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSAEMAENEDYICI
  NCAKKQGPDSPGQAPPPPFLMSYKLPMEDLKETS
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145997
• ORF Size: 5070 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145997.2, NP_666109.2
• RefSeq Size: 10969 bp
• RefSeq ORF: 5073 bp
• Locus ID: 214899
• UniProt ID: Q3UXZ9
• Cytogenetics: 6 56.95 cM
• MW: 192.2 kDa
• Gene Summary: Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4' (PubMed:17320161, PubMed:17320163). Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif. May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (By similarity). May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (PubMed:21960634). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (By similarity).[UniProtKB/Swiss-Prot Function]