Hspa1l (NM_013558) Mouse Tagged ORF Clone

CAT#: MR223206

Hspa1l (Myc-DDK-tagged) - Mouse heat shock protein 1-like (Hspa1l)

Product Images

Circular map for MR223206

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hspa1l
• Synonyms: Hsc70t; Msh5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223206 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTAATAAAGGAATGGCGATCGGCATCGACTTGGGCACCACCTACTCGTGCGTGGGCGTGTTCC
  AGCACGGCAAGGTGGAGATCATCGCCAACGACCAGGGCAACCGCACGACCCCTAGCTACGTGGCCTTCAC
  CGACACCGAGCGCCTCATCGGAGACGCTGCCAAGAACCAGGTGGCCATGAATCCCCAGAACACTGTTTTT
  GATGCCAAACGTCTAATTGGCAGGAAGTTTAATGATCCTGTTGTGCAGTCAGATATGAAGCTTTGGCCAT
  TTCAAGTGATCAATGAAGCCGGCAAACCCAAGGTGATGGTGTCCTATAAAGGAGAGAAGAAAGCCTTCTA
  CCCAGAGGAGATCTCATCCATGGTACTGACGAAGATGAAGGAGACTGCAGAGGCTTTTTTGGGCCACAAT
  GTCACCAACGCTGTGATCACGGTGCCAGCCTATTTCAATGACTCTCAGCGGCAAGCCACCAAGGATGCAG
  GTGTCATCGCAGGACTCAATGTGCTGAGAATAATCAATGAGCCCACGGCGGCCGCCATCGCCTACGGCTT
  GGATAAAGGAAGTCACGGAGAGCGGCACGTGCTCATCTTCGACCTGGGGGGTGGCACGTTCGACGTGTCC
  ATCCTGACGATCGACGACGGCATCTTCGAGGTGAAGGCCACGGCGGGCGACACGCACCTGGGAGGGGAGG
  ACTTCGACAACCGGCTGGTGAGCCACTTCGTGGAGGAGTTCAAGAGGAAGCACAAGAAGGACATCAGCCA
  GAACAAGCGCGCGGTGCGGCGGCTGCGCACTGCGTGTGAGAGGGCCAAGAGGACGCTGTCGTCCAGCACC
  CAGGCAAACCTGGAGATCGACTCTTTATATGAGGGCATCGACTTCTACACGTCCATCACTAGAGCACGGT
  TTGAAGAGCTGTGTGCAGACCTATTTAGAGGCACACTTGAGCCCGTGGAAAAGTCTCTTCGGGATGCCAA
  GATGGATAAGGCTAAAATCCATGACATTGTTCTAGTAGGGGGCTCCACCCGCATCCCAAAGGTGCAAAAA
  CTGCTTCAGGACTACTTTAATGGACGGGATCTCAACAAGAGTATCAATCCCGATGAGGCAGTCGCCTACG
  GAGCTGCAGTCCAGGCAGCTATTTTAATGGGCGACAAATCTGAAAAAGTACAGGATTTGCTTTTGTTGGA
  CGTAGCTCCCCTGTCTCTAGGATTGGAGACAGCTGGGGGTGTGATGACTGTACTGATCAAGCGCAACTCC
  ACCATCCCCACCAAGCAGACGCAGATCTTCACCACCTACTCGGACAACCAGCCCGGGGTGCTGATCCAGG
  TGTACGAGGGCGAGAGAGCCATGACGCGCGACAACAACCTGCTGGGGCGCTTTGACTTGACTGGAATACC
  TCCTGCACCTAGGGGCGTGCCACAGATCGAGGTGACCTTCGACATCGACGCCAACGGTATCCTGAACGTC
  ACGGCCATGGACAAGAGCACCGGCAAGGCCAACAAGATCACCATCACCAACGACAAGGGTCGCCTGAGCA
  AGGAGGAGATTGAGCGCATGGTGCAGGAGGCCGAGCGCTACAAAGCGGAGGATGAGGGCCAGAGGGAGAA
  AATCGCTGCCAAAAATGCCTTAGAATCGTACGCCTTTAATATGAAGAGCGCTGTGGGTGATGAGGGTCTG
  AAGGACAAGATCAGCGAGTCCGATAAAAAGAAAATACTGGATAAATGCAATGAGGTCCTTTCCTGGCTGG
  AGGCCAACCAGCTGGCTGAGAAAGATGAGTTTGATCATAAAAGAAAAGAACTGGAAAATATGTGTAATCC
  GATCATCACAAAACTGTACCAGAGCGGATGCACCGGGCCCACCTGTACGCCAGGGTATACTCCCGGCAGG
  GCTGCCACAGGCCCTACCATCGAGGAAGTAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223206 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAANKGMAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPQNTVF
  DAKRLIGRKFNDPVVQSDMKLWPFQVINEAGKPKVMVSYKGEKKAFYPEEISSMVLTKMKETAEAFLGHN
  VTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDKGSHGERHVLIFDLGGGTFDVS
  ILTIDDGIFEVKATAGDTHLGGEDFDNRLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSST
  QANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKSLRDAKMDKAKIHDIVLVGGSTRIPKVQK
  LLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNS
  TIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFDLTGIPPAPRGVPQIEVTFDIDANGILNV
  TAMDKSTGKANKITITNDKGRLSKEEIERMVQEAERYKAEDEGQREKIAAKNALESYAFNMKSAVGDEGL
  KDKISESDKKKILDKCNEVLSWLEANQLAEKDEFDHKRKELENMCNPIITKLYQSGCTGPTCTPGYTPGR
  AATGPTIEEVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013558
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013558.2, NP_038586.2
• RefSeq Size: 2415 bp
• RefSeq ORF: 1926 bp
• Locus ID: 15482
• UniProt ID: P16627
• Cytogenetics: 17 18.51 cM
• MW: 70.6 kDa
• Gene Summary: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Positive regulator of PRKN translocation to damaged mitochondria.[UniProtKB/Swiss-Prot Function]