Islr2 (NM_001161537) Mouse Tagged ORF Clone

CAT#: MR223162

Islr2 (Myc-DDK-tagged) - Mouse immunoglobulin superfamily containing leucine-rich repeat 2 (Islr2), transcript variant 3

Product Images

Circular map for MR223162

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Islr2
• Synonyms: B930052A04Rik; Linx; Mbu-3; mKIAA1465
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223162 representing NM_001161537
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCTTTGGAGCCCTGTGTTTGGCCTGGGCTTTGCTAGGAGTGGTCAGAGCGTGTCCCGAGCCTT
  GCGCCTGTGTTGACAAGTACGCCCACCAGTTTGCAGACTGTGCCTACAAGGAGCTGCGCGAGGTCCCAGA
  AGGACTGCCAGCCAACGTGACCACGCTTAGTCTGTCTGCCAACAAGATTACGGTACTAAGGCGGGGGGCC
  TTCGTCAACGTCACGCAGGTCACTTCGCTGTGGCTGGCTCACAGTGAGGTACGCACGGTAGAGTCAGGGG
  CATTGGCAGTGCTGAGTCAGCTCAAGAACCTCGACCTAAGCCACAACCTTATATCCAACTTCCCTTGGAG
  CGACCTTCGTAACTTGAGCGCGCTGCAGCTGCTGAAAATGAACCACAACCGTCTGGGATCGCTGCCCCGG
  GATGCACTCGGCGCGCTCCCGGACCTGCGCTCTCTGCGCATCAACAACAACCGGCTGCGTACCCTGGAGC
  CCGGCACGTTCGACGCACTAAGCGCGCTGTCTCACCTGCAACTCTATCACAACCCCTTCCACTGCAGCTG
  TGGTCTCGTGTGGCTGCAGGCCTGGGCAGCGAGCACCCGGGTCTCCTTACCGGAGCCCGATTCTATAGCG
  TGCGCCTCGCCCCCTGAGCTGCAGGGCGTGCCCGTGCACCGCCTGCCCGCCCTGCCCTGCGCACCCCCCA
  GCGTGCGTCTGAGCGCAGAGCCGCCGCCTGAGGCACCTGGCACCCCTCTGCGCGCAGGCTTGGCTTTCAT
  GTTACATTGCGTCGCCGAAGGCCACCCCACACCCCGCCTGCAATGGCAACTTCAGATCCCGGGTGGCACT
  GTAGTCTTAGTGCCACCGGTTCTCAGCAAGGAAGAAGATGGAGGAGATAAGGTGGAGGATGGAGAGGGTG
  ACGGAGATGAGGACCTGCCTACGCAGACTGAAGCGCCAACCCCAACTCCAGCACCTGCTTGGCCAGCGCC
  TCCAGCCACCCCGCGCTTCTTGGCCCTCGCAAATGGCTCTCTGTTGGTGCCCCTTCTGAGTGCCAAGGAG
  GCAGGCATCTACACTTGTCGTGCACACAATGAGCTGGGCACCAACTCAACGTCCTTACGGGTGACGGTGG
  CTGCAGCGGGGCCGCCAAAACACGCTCCTGGAACAGGGGAAGAACCTGATGCGCAGGTCCCGACCTCTGA
  GCGCAAGGCCACCACTAAGGGCCGTAGCAACAGCGTCCTGCCCTTCAAGCCTGAAGGCAAAACCAAAGGC
  CAAGGTCTTGCCCGAGTCAGCGTGCTTGGGGAAATCGAGGCAGAGCTGGAGGAGACAGATGAAGGAGAGC
  AGATGGAAGGTCAGATCCCTGCAGATCCGATGGGAGAGAAGCACTGTGGCCATGGGGACCCCTCTCGCTA
  TGTGTCTAACCATGCATTCAACCAGAGCTCAGATCTCAAGCCGCACGTTTTTGAGTTGGGCGTCATCGCG
  CTGGATGTAGCAGAGCGTGAGGCTCGGGTGCAGCTGACGCCTCTTGCTGCGCGCTGGGGCCCTGGGCCAG
  ATGGTGCTAGCGGAGCGCGGCGGCCGGGGAGGCGGCCACTGCGCCTACTCTATCTGTGTCCTGCGGGGGG
  TGGCACGGCAGTTCAGTGGTCACGAGTGGAAGAGGGGGTCAATGCCTACTGGTTTCGCGGCCTGCGGCCT
  GGCACCAACTACTCCGTATGCCTGGCACTGGCGGGCGAGGCGTGTCACGTGCAGGTGGTGTTTTCTACCA
  AGAAAGAACTGCCGTCCCTGCTGGTTATCGTGACCGTGAGTGTTTTTCTCCTGGTGCTGGCCACCGTGCC
  CCTGCTGGGTGCCGCCTGCTGCCATCTGCTGGCCAAACATCCGGGCAAACCCTACCGTTTAATCCTGAGG
  CCACAGGCCCCGGACCCTATGGAGAAACGCATCGCGGCCGATTTCGATCCGCGTGCTTCCTACCTTGAGT
  CTGAGAAAAGCTACCCTGCTCGTGGCGAGGCGGGAGGTGAGGAGCCAGAGGAGGTCCCGGAGGAAGGCCT
  GGATGAAGATGTGGAGCAGGGGGACCCAAGCGGGGACCTTCAGAGAGAGGAAAGCCTGGCGGGTTGCTCA
  TTGGTGGAGTCTCAGTCCAAGGCCAACCAAGAGGAGTTCGAGGCTGGCTCGGAGTACAGCGATCGGCTGC
  CCCTGGGAGCGGAAGCAGTCAACATCGCCCAGGAAATAAACGGCAACTACAGGCAGACAGCGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223162 representing NM_001161537
  Red=Cloning site Green=Tags(s)
  
  MGPFGALCLAWALLGVVRACPEPCACVDKYAHQFADCAYKELREVPEGLPANVTTLSLSANKITVLRRGA
  FVNVTQVTSLWLAHSEVRTVESGALAVLSQLKNLDLSHNLISNFPWSDLRNLSALQLLKMNHNRLGSLPR
  DALGALPDLRSLRINNNRLRTLEPGTFDALSALSHLQLYHNPFHCSCGLVWLQAWAASTRVSLPEPDSIA
  CASPPELQGVPVHRLPALPCAPPSVRLSAEPPPEAPGTPLRAGLAFMLHCVAEGHPTPRLQWQLQIPGGT
  VVLVPPVLSKEEDGGDKVEDGEGDGDEDLPTQTEAPTPTPAPAWPAPPATPRFLALANGSLLVPLLSAKE
  AGIYTCRAHNELGTNSTSLRVTVAAAGPPKHAPGTGEEPDAQVPTSERKATTKGRSNSVLPFKPEGKTKG
  QGLARVSVLGEIEAELEETDEGEQMEGQIPADPMGEKHCGHGDPSRYVSNHAFNQSSDLKPHVFELGVIA
  LDVAEREARVQLTPLAARWGPGPDGASGARRPGRRPLRLLYLCPAGGGTAVQWSRVEEGVNAYWFRGLRP
  GTNYSVCLALAGEACHVQVVFSTKKELPSLLVIVTVSVFLLVLATVPLLGAACCHLLAKHPGKPYRLILR
  PQAPDPMEKRIAADFDPRASYLESEKSYPARGEAGGEEPEEVPEEGLDEDVEQGDPSGDLQREESLAGCS
  LVESQSKANQEEFEAGSEYSDRLPLGAEAVNIAQEINGNYRQTAG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161537
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161537.1, NP_001155009.1
• RefSeq Size: 4310 bp
• RefSeq ORF: 2238 bp
• Locus ID: 320563
• UniProt ID: Q5RKR3
• Cytogenetics: 9 B
• MW: 80.2 kDa
• Gene Summary: Required for axon extension during neural development.[UniProtKB/Swiss-Prot Function]