Cask (NM_009806) Mouse Tagged ORF Clone

CAT#: MR223129

Cask (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent serine protein kinase (MAGUK family) (Cask)

Product Images

Circular map for MR223129

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cask
• Synonyms: DXPri1; DXRib1; LIN-2; mLin-2; Pals3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223129 representing NM_009806
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACGACGACGTGCTGTTCGAGGATGTGTACGAGCTATGCGAGGTGATCGGCAAGGGTCCCTTCA
  GTGTTGTACGGCGATGTATCAACAGAGAAACTGGGCAACAATTTGCTGTAAAAATTGTTGATGTAGCCAA
  GTTCACATCAAGTCCAGGGTTAAGTACAGAAGATCTAAAGCGGGAAGCCAGTATCTGTCATATGCTGAAG
  CATCCACACATTGTAGAGCTGTTGGAGACATATAGCTCAGATGGGATGCTTTACATGGTTTTTGAATTTA
  TGGATGGAGCAGATCTGTGTTTTGAAATCGTAAAGCGAGCTGATGCTGGTTTTGTATACAGTGAAGCTGT
  AGCCAGCCACTACATGAGACAGATACTGGAAGCTCTGCGCTACTGTCATGATAATAACATAATTCACAGG
  GACGTGAAGCCCCACTGTGTTCTCCTTGCCTCAAAAGAAAACTCGGCACCTGTTAAACTTGGGGGCTTTG
  GGGTGGCCATTCAGTTAGGAGAATCTGGACTTGTTGCTGGAGGCCGCGTTGGAACACCTCACTTTATGGC
  CCCAGAAGTGGTCAAGAGAGAGCCTTACGGAAAGCCTGTGGATGTCTGGGGCTGTGGTGTGATCCTTTTC
  ATCCTGCTCAGTGGCTGTTTGCCTTTCTACGGAACCAAGGAAAGATTGTTTGAAGGCATTATTAAAGGAA
  AATATAAGATGAATCCAAGGCAGTGGAGCCATATCTCTGAAAGTGCCAAAGACCTAGTACGCCGCATGCT
  GATGCTGGATCCTGCTGAAAGGATCACTGTTTATGAAGCACTGAATCACCCATGGCTTAAGGAGCGGGAT
  CGTTATGCCTACAAAATCCATCTTCCAGAAACAGTAGAACAACTGAGGAAATTCAATGCAAGGAGAAAAC
  TAAAGGGTGCAGTACTAGCTGCTGTGTCAAGTCACAAATTCAATTCCTTCTATGGGGACCCTCCTGAAGA
  GTTGCCAGATTTCTCCGAAGACCCTACCTCCTCAGGAGCCGTCTCTCAGGTGCTGGACAGCCTGGAAGAG
  ATTCACGCACTTACAGACTGCAGTGAAAAGGACCTAGATTTTCTACACAGTGTTTTCCAGGATCAACATC
  TTCACACACTGCTGGATCTGTATGACAAAATTAACACAAAGTCTTCGCCACAAATCAGGAATCCTCCAAG
  TGATGCAGTACAGAGAGCCAAAGAGGTATTGGAAGAAATTTCATGTTACCCTGAGAATAATGATGCGAAG
  GAACTAAAGCGTATTTTAACACAACCTCATTTCATGGCCTTACTTCAGACTCATGATGTAGTGGCACATG
  AAGTTTACAGTGATGAAGCATTAAGGGTCACACCTCCCCCGACTTCCCCCTATTTAAACGGTGATTCTCC
  AGAAAGTGCAAACGGAGACATGGACATGGAGAATGTGACCAGAGTTCGGCTGGTACAGTTCCAAAAGAAC
  ACGGATGAGCCAATGGGAATCACTTTGAAAATGAATGAGCTAAATCATTGTATTGTCGCAAGAATCATGC
  ATGGGGGTATGATTCACAGGCAAGGTACACTTCATGTTGGTGATGAAATCCGAGAAATCAATGGCATCAG
  TGTCGCTAACCAAACAGTGGAACAGCTACAGAAAATGCTTAGGGAAATGCGAGGGAGTATTACCTTCAAG
  ATTGTGCCAAGCTACCGCACTCAGTCTTCGTCCTGTGAGGACTTGCCATCAACCACCCAACCAAAAGGAC
  GACAGATCTATGTAAGAGCACAATTTGAATATGATCCAGCCAAGGATGACCTCATCCCCTGCAAAGAAGC
  TGGCATCCGGTTCCGAGTTGGTGACATCATCCAGATTATTAGTAAGGATGACCACAACTGGTGGCAGGGT
  AAACTGGAAAACTCCAAAAATGGAACTGCAGGTCTCATTCCTTCTCCTGAACTTCAGGAATGGCGAGTAG
  CTTGCATTGCCATGGAGAAGACCAAACAAGAGCAGCAGGCCAGCTGTACTTGGTTTGGCAAGAAAAAGAA
  GCAGTACAAAGATAAATATTTGGCAAAGCACAACGCAGTGTTTGATCAATTAGATCTTGTCACATATGAA
  GAAGTAGTCAAACTGCCAGCATTCAAAAGGAAAACATTAGTCTTATTAGGTGCACATGGTGTTGGAAGAA
  GACACATAAAAAATACCCTCATCACAAAGCACCCGG[AT]CCGGTTTGCGTACCCTATTCCACATACAAC
  CAGACCTCCAAAGAAAGATGAAGAAAATGGCAAGAATTATTACTTTGTATCTCATGACCAAATGATGCAA
  GACATCTCAAATAACGAATACTTGGAGTATGGCAGCCATGAGGATGCAATGTACGGGACAAAACTGGAGA
  CCATTCGGAAAATCCATGAGCAGGGGCTGATTGCGATTCTGGACGTGGAGCCTCAGGCACTGAAGGTCCT
  GAGGACTGCAGAGTTTGCTCCTTTTGTTGTCTTCATTGCGGCGCCAACTATCACTCCAGGTTTAAATGAG
  GATGAATCTCTTCAGCGCCTGCAGAAGGAGTCCGATGTCTTGCAGAGAACATATGCACACTACTTCGATC
  TCACAATTATCAACAACGAAATTGATGAGACAATCAGACATCTGGAAGAAGCTGTCGAGCTTGTGTGCAC
  AGCCCCACAGTGGGTCCCAGTCTCCTGGGTCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223129 representing NM_009806
  Red=Cloning site Green=Tags(s)
  
  MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLK
  HPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHR
  DVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILF
  ILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERD
  RYAYKIHLPETVEQLRKFNARRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGAVSQVLDSLEE
  IHALTDCSEKDLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRNPPSDAVQRAKEVLEEISCYPENNDAK
  ELKRILTQPHFMALLQTHDVVAHEVYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVTRVRLVQFQKN
  TDEPMGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVANQTVEQLQKMLREMRGSITFK
  IVPSYRTQSSSCEDLPSTTQPKGRQIYVRAQFEYDPAKDDLIPCKEAGIRFRVGDIIQIISKDDHNWWQG
  KLENSKNGTAGLIPSPELQEWRVACIAMEKTKQEQQASCTWFGKKKKQYKDKYLAKHNAVFDQLDLVTYE
  EVVKLPAFKRKTLVLLGAHGVGRRHIKNTLITKHPXXRFAYPIPHTTRPPKKDEENGKNYYFVSHDQMMQ
  DISNNEYLEYGSHEDAMYGTKLETIRKIHEQGLIAILDVEPQALKVLRTAEFAPFVVFIAAPTITPGLNE
  DESLQRLQKESDVLQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVPVSWVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009806
• ORF Size: 2694 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009806.2, NM_009806.3, NP_033936.2
• RefSeq Size: 8260 bp
• RefSeq ORF: 2694 bp
• Locus ID: 12361
• UniProt ID: O70589
• Cytogenetics: X 8.43 cM
• MW: 102.5 kDa
• Gene Summary: Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.[UniProtKB/Swiss-Prot Function]