Cdh7 (NM_172853) Mouse Tagged ORF Clone

CAT#: MR223112

Cdh7 (Myc-DDK-tagged) - Mouse cadherin 7, type 2 (Cdh7)

Product Images

Circular map for MR223112

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdh7
• Synonyms: 9330156F07Rik; CDH7L; CDH7L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223112 representing NM_172853
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGGGCAAAGTGGAGTTGTGCCATTTTCTACAGCTTATAGCTCTCTTCCTTTGCTTCTCTGGAA
  TGAGTCAAGCTGAGCTGCCACGGTCCAGATCCAAGCCATACTTCCAGTCAGGAAGGTCCCGGACCAAGCG
  CAGCTGGGTTTGGAATCAGTTCTTCGTGCTGGAAGAATACATGGGTTCAGATCCCCTCTATGTAGGGAAG
  CTCCACTCTGATGTTGATAAAGGAGACGGTTCCATCAAGTACATCTTGTCAGGCGAAGGGGCAAGTTCAA
  TTTTCATTATTGATGAGAACACTGGTGATATCCATGCTACCAAGAGACTGGATCGGGAGGAGCAGGCCTA
  TTATACACTGAGAGCCCAAGCTCTGGACAGACTCACCAACAAGCCTGTGGAGCCTGAATCAGAGTTTGTT
  ATCAAGATTCAGGATATCAATGACAATGAACCCAAATTTCTAGATGGTCCATACACAGCAGGAGTTCCTG
  AGATGTCTCCTGTAGGGACTTCAGTGGTACAAGTGACAGCAACAGATGCCGATGATCCTACATATGGCAA
  CAGTGCTCGAGTGGTGTATAGTATTCTTCAAGGACAGCCATATTTCTCAGTGGAACCCAAAACAGGAGTC
  ATCAAGACTGCCCTTCCAAACATGGATAGAGAGGCTAAAGACCAGTATTTGCTTGTCATTCAGGCAAAGG
  ATATGGTTGGTCAAAATGGCGGACTGTCTGGAACCACCTCAGTTACTGTGACCCTAACTGATGTCAATGA
  TAACCCACCTCGATTTCCACGAAGATCTTATCAGTATAATGTTCCAGAATCTTTGCCTGTGGCATCAGTT
  GTGGCCAGAATTAAAGCTGCAGATGCAGATATAGGAGTCAATGCAGAAATGGAATACAAAATAGTGGATG
  GTGATGGACTGGGCATTTTCAAGATATCTGCTGACAAAGACACACAAGAAGGAATCATTACAATACAAAA
  GGAACTCGATTTTGAAGCCAAAACGAGTTATACACTGCGCATAGAAGCTGCAAATCGAGATGCTGATCCC
  CGGTTTCTGAGCTTGGGTCCATTCAGTGACACAACAACGGTTAAGATAATTGTGGAAGATGTGGATGAGC
  CCCCTGTTTTTTCTTCACCCTTGTATCCCATGGAGGTGTCAGAAGCGACACAGGTGGGGCATATCATTGG
  CACTGTAGCAGCTCATGACCCAGATTCTTCCAACAGCCCTGTGAGATATTCAATTGACAGAAACACAGAC
  TTGGAGAGATATTTCAATATTGATGCCAACAGTGGAGTTATTACTACTGCCAAATCATTGGATCGAGAGA
  CAAATGCGGTTCATAACATTACAGTCCTTGCAATGGAGAGCCAGAATCCATCCCAGGTGGGGAGAGGCTA
  CGTGGCCATTACTATACTGGATATTAACGACAACGCACCTGAGTTCGCCATGGACTATGAAACCACTGTC
  TGTGAAAACGCTCAGCCAGGGCAGGTTATCCAGAAAATCAGTGCAGTAGATAAAGATGAGCCATCCAATG
  GACACCAGTTTTACTTCAGCTTAACAACAGACATGACAAATAACCACAACTTTTCATTGAAAGATAACAA
  AGACAATACAGCCTCAATACTCACGAGAAGAAATGGCTTCCGAAGACAGGAACAATCAGTTTATTATCTG
  CCCATTTTCATTGTGGACAGTGGATCTCCTTCACTAAGCAGTACCAACACACTTACCATCCGTGTATGTG
  ACTGTGATGCTGATGGCATAGCCCAGACCTGCAATGCAGAGGCCTATGTTCTACCTGCTGGCCTCAGCAC
  TGGGGCCTTGATAGCAATACTGGCCTGTGTCCTGACACTATTGGTGCTGATCCTCCTCATTGTCACTATG
  AGAAGACGAAAAAAAGAGCCTCTCATTTTTGATGAGGAGAGGGATATCAGAGAAAACATAGTCAGATATG
  ATGATGAAGGCGGGGGAGAAGAAGACACAGAAGCCTTTGACATGGCTGCGCTGAGAAACCTCAATGCCAT
  AAGAGACAGCAAAACTCGGAGAGATGTGACTCCTGAAATTCAGTTCTTGAGTCGACCGACTTTTAAAAAC
  ATCCCAGATAATGTCATTTTTAGGGAATTTATTTGGGAAAGACTGAAAGAAGCTGATGTGGATCCAGGGG
  CTCCACCATATGACTCTCTTCAGACTTATGCATTTGAAGGAAATGGCTCAGTAGCTGAATCTCTCAGTTC
  TTTAGATTCCATCAGCTCAAACTCTGATCAGAATTATGACTACCTTAGTGACTGGGGTCCCCGTTTTAAA
  CGACTAGCTGAAATGTATGGAAATGGCCAAGAGAGTTTGTACTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223112 representing NM_172853
  Red=Cloning site Green=Tags(s)
  
  MKLGKVELCHFLQLIALFLCFSGMSQAELPRSRSKPYFQSGRSRTKRSWVWNQFFVLEEYMGSDPLYVGK
  LHSDVDKGDGSIKYILSGEGASSIFIIDENTGDIHATKRLDREEQAYYTLRAQALDRLTNKPVEPESEFV
  IKIQDINDNEPKFLDGPYTAGVPEMSPVGTSVVQVTATDADDPTYGNSARVVYSILQGQPYFSVEPKTGV
  IKTALPNMDREAKDQYLLVIQAKDMVGQNGGLSGTTSVTVTLTDVNDNPPRFPRRSYQYNVPESLPVASV
  VARIKAADADIGVNAEMEYKIVDGDGLGIFKISADKDTQEGIITIQKELDFEAKTSYTLRIEAANRDADP
  RFLSLGPFSDTTTVKIIVEDVDEPPVFSSPLYPMEVSEATQVGHIIGTVAAHDPDSSNSPVRYSIDRNTD
  LERYFNIDANSGVITTAKSLDRETNAVHNITVLAMESQNPSQVGRGYVAITILDINDNAPEFAMDYETTV
  CENAQPGQVIQKISAVDKDEPSNGHQFYFSLTTDMTNNHNFSLKDNKDNTASILTRRNGFRRQEQSVYYL
  PIFIVDSGSPSLSSTNTLTIRVCDCDADGIAQTCNAEAYVLPAGLSTGALIAILACVLTLLVLILLIVTM
  RRRKKEPLIFDEERDIRENIVRYDDEGGGEEDTEAFDMAALRNLNAIRDSKTRRDVTPEIQFLSRPTFKN
  IPDNVIFREFIWERLKEADVDPGAPPYDSLQTYAFEGNGSVAESLSSLDSISSNSDQNYDYLSDWGPRFK
  RLAEMYGNGQESLYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172853
• ORF Size: 2355 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172853.3, NP_766441.1
• RefSeq Size: 3353 bp
• RefSeq ORF: 2358 bp
• Locus ID: 241201
• UniProt ID: Q8BM92
• Cytogenetics: 1 E2.1
• MW: 87.7 kDa
• Gene Summary: This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene is located adjacent to a related cadherin gene on chromosome 1. [provided by RefSeq, Oct 2015]