Emx1 (NM_010131) Mouse Tagged ORF Clone

CAT#: MR222943

Emx1 (Myc-DDK-tagged) - Mouse empty spiracles homolog 1 (Drosophila) (Emx1)

Product Images

Circular map for MR222943

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Emx1
• Synonyms: MGC129284; MGC129285
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222943 representing NM_010131
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCAGCCCGCGGCTAAGCGGGGTTTCACCATAGAGTCCTTGGTGGCCAAGGATGGTGGCACCGGCG
  GGAGTCCTGGCAGCGGGGGCGCGGGCTCCCATCCACTGGCTGTGGCCGCATCAGAGGAACCGCTCAGGCC
  CACGGCGCTCAACTATCCTCACCCCAGTGCGGCCGAGACGGCCTTCGTGAGTGGCTTCCCTGCCGCTGCC
  GCTGCGGGCGCCGGCCGCTCGCTCTATGGAGGGCCGGAGCTGGTGTTCCCAGAGGCCATGAACCACCCGG
  CTCTGACGGTGCACCCGGCACACCAGCTGGGCTCCTCCTCGCTGCAACCCCCTCACTCTTTCTTCAGCGC
  CCAGCATCGGGACCCTCTCCACTTCTACCCCTGGGTGCTTCGGAATCGCTTCTTTGGCCACCGCTTCCAG
  GCAAGCGACGTTCCCCAGGACGGGCTGCTTTTGCACGGGCCCTTCGCACGCAAGCCCAAGCGGATTCGCA
  CAGCCTTCTCGCCCTCGCAGCTGCTGCGGCTGGAGCGAGCCTTTGAGAAGAATCACTACGTGGTGGGAGC
  CGAGCGGAAGCAGCTGGCAGGCAGCCTCAGCCTCTCCGAGACGCAGGTGAAGGTGTGGTTCCAGAATCGG
  AGGACAAAATACAAACGGCAGAAGCTGGAAGAGGAAGGGCCGGAGTCTGAGCAGAAGAAGAAGGGTTCCC
  ACCATATCAACCGGTGGCGCATCGCCACGAAGCAGGCCAACGGGGAGGACATTGATGTCACCTCCAATGA
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222943 representing NM_010131
  Red=Cloning site Green=Tags(s)
  
  MFQPAAKRGFTIESLVAKDGGTGGSPGSGGAGSHPLAVAASEEPLRPTALNYPHPSAAETAFVSGFPAAA
  AAGAGRSLYGGPELVFPEAMNHPALTVHPAHQLGSSSLQPPHSFFSAQHRDPLHFYPWVLRNRFFGHRFQ
  ASDVPQDGLLLHGPFARKPKRIRTAFSPSQLLRLERAFEKNHYVVGAERKQLAGSLSLSETQVKVWFQNR
  RTKYKRQKLEEEGPESEQKKKGSHHINRWRIATKQANGEDIDVTSND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010131
• ORF Size: 771 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010131.2, NP_034261.1
• RefSeq Size: 1188 bp
• RefSeq ORF: 774 bp
• Locus ID: 13796
• UniProt ID: Q04742
• Cytogenetics: 6 37.21 cM
• MW: 28.6 kDa
• Gene Summary: Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.[UniProtKB/Swiss-Prot Function]