Cacna1d (NM_028981) Mouse Tagged ORF Clone

CAT#: MR222925

Cacna1d (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 1

Product Images

Circular map for MR222925

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1d
• Synonyms: 8430418G19Rik; C79217; Cach3; Cacn; Cacn4; Cacnl1a2; Cav1.3; Cchl; Cchl1; Cchl1a; Cchl1a2; D-LTC; D-LTCC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222925 representing NM_028981
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTTCCGACATTTTCTAGTGATCTAATTTTAATTAAAAGCGTTCTCTCCCAAGAGACTGATGCCC
  GATATAAAGGCAGAGTTGTAAGTGCGGTAGAAAGCACGGAGGACTTCTCCCAGGCCTTTGCAGAGGCAAA
  CTATGCAAGAGGCACCAGACTCCCTATTTCTGGTGAAGGACCAACTTCTCAGCCCAACAGCTCCAAGCAA
  ACTGTCCTATCTTGGCAAGCTGCAATCGATGCTGCTAGACAGGCCAAGGCCGCCCAGACCATGAGCACCT
  CTGCACCCCCACCTGTAGGATCTCTCTCCCAAAGAAAACGTCAGCAATACGCCAAGAGCAAAAAACAGGG
  TAACTCGTCTAACAGCCGACCTGCCCGTGCCCTTTTCTGTTTATCCCTCAATAACCCCATCCGAAGAGCC
  TGCATTAGTATAGTGGAATGGAAACCATTTGACATATTTATATTATTGGCTATTTTTGCCAATTGTGTGG
  CCTTAGCTATTTACATCCCATTCCCTGAAGATGATTCTAATTCAACAAATCATAACTTGGAAAAAGTAGA
  ATATGCCTTCCTGATTATTTTTACAGTGGAGACATTTCTGAAGATTATAGCGTATGGACTGTTGCTGCAT
  CCTAATGCTTACGTTAGGAATGGATGGAATTTACTGGATTTTGTTATAGTAATTGTAGGATTGTTTAGTG
  TAATTCTGGAACAATTAACCAAAGAAACAGAAGGCGGGAACCACTCTAGTGGCAAATCAGGAGGCTTTGA
  TGTCAAAGCCCTCCGTGCCTTCCGAGTGTTAAGACCACTTCGACTTGTATCAGGAGTGCCCAGTTTACAA
  GTTGTTCTGAACTCCATTATAAAAGCCATGGTCCCCCTCCTTCACATAGCCCTTTTGGTATTATTTGTAA
  TTATCATCTATGCTATTATAGGATTGGAACTTTTTATTGGAAAAATGCACAAAACATGTTTTTTTGCTGA
  CTCAGATATAGTAGCTGAAGAGGACCCAGCTCCGTGTGCGTTCTCAGGGAATGGACGCCAGTGCACCGCC
  AATGGCACGGAATGTAGGAGTGGCTGGGTTGGCCCCAATGGAGGCATCACTAACTTTGATAACTTTGCCT
  TTGCCATGCTCACTGTGTTCCAGTGCATTACCATGGAGGGCTGGACAGATGTGCTCTACTGGGTTAATGA
  TGCGATAGGATGGGAATGGCCATGGGTGTATTTTGTTAGTCTGATCATCCTTGGCTCATTTTTCGTCCTT
  AACCTGGTTCTTGGTGTCCTTAGTGGAGAATTCTCCAAGGAAAGAGAGAAGGCTAAAGCTCGTGGAGATT
  TCCAGAAGCTTCGGGAAAAACAGCAGCTGGAAGAGGACCTCAAGGGCTACCTGGACTGGATCACCCAGGC
  AGAAGACATCGATCCCGAGAACGAGGAGGAGGGTGGCGAGGAAGGCAAACGAAACACTAGCATGCCCACC
  AGCGAGACTGAATCTGTCAACACAGAGAACGTAAGTGGTGAAGGCGAGACCCAGGGATGCTGTGGAACTC
  TCTGTCAGGCCATCTCGAAATCCAAACTCAGCCGTCGATGGCGTCGCTGGAACCGGTTCAATCGCAGGAG
  GTGCAGGGCTGCCGTGAAGTCTGTCACGTTTTACTGGCTGGTGATTGTCCTGGTGTTTCTCAACACTTTA
  ACTATTTCCTCTGAGCATTACAATCAGCCAGACTGGCTGACACAGATTCAAGACATCGCAAACAAAGTCC
  TCTTGGCTCTGTTCACCTGTGAGATGCTGGTGAAGATGTACAGCCTGGGCCTCCAGGCTTACTTTGTCTC
  CCTCTTCAACCGCTTCGATTGCTTTGTGGTGTGTGGGGGCATCACCGAAACCATCCTGGTGGAACTGGAG
  CTCATGTCTCCCCTGGGGGTCTCCGTGTTCCGGTGTGTGCGCCTCCTGAGAATCTTCAAAGTGACCAGGC
  ACTGGACCTCCCTGAGCAACTTGGTGGCATCCTTGTTAAACTCCATGAAGTCCATCGCCTCTCTGCTGTT
  ACTCCTCTTCCTCTTCATCATCATCTTCTCCTTGCTGGGGATGCAGTTGTTTGGTGGGAAGTTTAATTTT
  GACGAGACTCAAACCAAGCGAAGCACCTTTGACAACTTTCCGCAAGCACTCCTGACGGTGTTCCAGATCC
  TGACAGGTGAAGACTGGAATGCGGTGATGTATGACGGCATCATGGCCTATGGAGGCCCTTCCTCTTCCGG
  GATGATTGTCTGTATCTACTTCATCATCCTTTTCATCTGTGGTAACTATATCCTGCTGAATGTCTTCTTG
  GCCATCGCTGTGGACAATTTGGCTGATGCTGAAAGTCTGAACACTGCTCAGAAAGAGGAAGCTGAAGAAA
  AAGAAAGGAAAAAGATTGCCAGAAAAGAAAGCCTAGAAAACAAAAAGAACAACAAACCAGAAGTCAACCA
  GATAGCCAACAGTGACAACAAGGTTACAATTGATGATTATCAGGAAGATGCTGAAGACAAGGACCCTTAC
  CCGCCCTGTGATGTGCCAGTAGGTGAAGAGGAAGAGGAGGAGGAAGAGGATGAGCCCGAAGTTCCTGCTG
  GTCCCCGCCCTCGCAGAATCTCAGAGTTGAACATGAAGGAGAAGATTGCGCCCATCCCAGAAGGAAGCGC
  CTTCTTCATTCTTAGCAAGACCAACCCGATACGTGTGGGTTGCCACAAGCTCATTAACCACCACATCTTC
  ACCAACCTTATCCTGGTCTTCATCATGCTGAGCAGCGCTGCCCTGGCTGCAGAGGACCCCATCCGTAGCC
  ACTCATTCCGCAACACGATACTGGGCTACTTTGACTATGCCTTCACAGCCATCTTTACGGTTGAAATCCT
  GTTAAAGATGACAACTTTTGGAGCCTTCTTGCACAAAGGGGCTTTCTGCAGGAACTACTTCAATTTGCTG
  GACATGCTGGTTGTTGGGGTGTCGCTGGTGTCCTTTGGGATTCAGTCCAGCGCCATCTCCGTTGTGAAGA
  TTCTGAGGGTTTTGAGGGTCTTGCGGCCTCTCAGAGCAATCAACAGAGCAAAGGGACTTAAGCACGTGGT
  CCAGTGTGTCTTTGTGGCCATCCGAACCATTGGCAACATCATGATCGTCACGACCCTGCTCCAGTTCATG
  TTTGCATGCATTGGGGTCCAGCTGTTCAAGGGGAAGTTCTACCGTTGCACAGATGAAGCCAAAAGTAACC
  CTGAGGAGTGCAGGGGGCTTTTCATTCTTTATAAGGACGGCGATGTTGACAGTCCTGTGGTCCGTGAGAG
  GATCTGGCAAAATAGTGATTTCAATTTTGACAATGTCCTTTCGGCTATGATGGCGCTGTTCACAGTCTCA
  ACTTTTGAGGGCTGGCCCGCGTTGCTGTACAAAGCTATTGATTCAAACGGTGAGAACGTTGGTCCTGTCT
  ACAACTACCGTGTGGAGATCTCCATCTTCTTCATCATCTACATCATCATCGTTGCCTTCTTCATGATGAA
  CATCTTTGTGGGCTTCGTCATCGTCACCTTCCAGGAACAGGGAGAAAAAGAGTATAAGAACTGTGAGCTG
  GACAAAAATCAGCGTCAGTGTGTTGAATATGCCTTGAAAGCACGCCCCTTAAGGAGATACATCCCCAAAA
  ACCCATACCAGTACAAGTTCTGGTATGTGGTGAACTCCTCGCCTTTCGAATACATGATGTTCGTCCTCAT
  CATGCTCAACACGCTCTGCCTGGCCATGCAGCACTATGAGCAGTCCAAGATGTTCAATGATGCCATGGAC
  ATTCTGAACATGGTCTTCACAGGGGTCTTCACTGTTGAGATGGTTTTGAAAGTCATTGCTTTCAAGCCCA
  AGGGGTATTTTAGTGACGCCTGGAACACGTTTGACTCCCTCATCGTAATCGGCAGCATTATAGACGTGGC
  CCTCAGCGAAGCCGACAACTCTGAAGAGAGCAATAGAATCTCCATCACCTTTTTCCGTCTTTTCCGAGTG
  ATGCGGTTGGTGAAACTTCTCAGCAGGGGGGAAGGCATCCGGACACTGCTGTGGACCTTCATCAAGTCCT
  TTCAGGCGCTGCCGTATGTTGCCCTCCTCATTGCCATGCTCTTCTTCATCTACGCTGTCATAGGCATGCA
  GATGTTTGGGAAAGTTGCCATGAGAGATAACAACCAGATCAATAGGAACAACAACTTCCAGACCTTTCCT
  CAGGCAGTGCTCCTACTCTTCAGGTGTGCAACAGGGGAAGCCTGGCAGGAGATCATGCTTGCCTGCCTCC
  CAGGAAAGCTGTGCGACCCAGACTCCGATTACAACCCAGGAGAGGAGTACACTTGTGGGAGCAACTTTGC
  CATTGTCTACTTCATCAGCTTTTACATGCTCTGTGCATTCCTGATCATCAACCTCTTTGTGGCTGTCATC
  ATGGACAATTTTGACTATCTGACTCGGGACTGGTCTATTCTGGGGCCTCACCACTTGGACGAATTCAAAA
  GAATATGGTCAGAATATGACCCTGAAGCAAAGGGAAGGATAAAACACCTTGATGTGGTCACTCTGCTCCG
  GCGGATCCAGCCTCCCCTGGGGTTTGGAAAATTATGCCCACACCGAGTAGCATGTAAGAGATTGGTTGCC
  ATGAACATGCCTCTCAACAGTGATGGGACAGTCATGTTCAATGCAACCCTGTTTGCTTTGGTCCGGACAG
  CTCTCAAGATCAAGACTGAAGGGAACCTGGAGCAAGCTAATGAAGAACTCCGAGCTGTGATCAAGAAAAT
  CTGGAAGAAGACAAGCATGAAGCTGCTTGACCAAGTTGTCCCTCCAGCTGGTGATGATGAGGTAACCGTG
  GGGAAGTTCTATGCCACTTTCCTGATACAGGACTACTTTAGGAAATTCAAGAAACGGAAAGAGCAAGGAC
  TGGTGGGGAAGTACCCTGCGAAGAACACCACGATTGCCCTACAGGCGGGATTAAGGACCCTGCATGACAT
  TGGGCCAGAAATCCGACGGGCTATATCCTGTGACTTGCAAGATGACGAGCCAGAAGACTCCAAACCAGAA
  GAAGAAGATGTATTCAAAAGAAATGGTGCCCTGCTTGGAAACCATGTCAATCATGTTAATAGTGATAGGA
  GAGATTCCCTTCAGCAGACCAATACCACCCACCGTCCCCTGCATGTCCAAAGGCCTTCAATGCCACCTGC
  AAGTGATACTGAGAAACCGCTGTTTCCTCCAGCAGGAAATTCGGGGTGTCATAACCATCATAACCATAAT
  TCCATAGGGAAGCAAGCTCCCACCTCAACAAATGCCAATCTCAATAATGCCAATATGTCCAAAGCTGCCC
  ACGGAAAGCCGCCCAGCATTGGGAACCTTGAGCATGTGTCTGAAAATGGGCATTACTCCTGCAAGCATGA
  TCGAGAGCTTCAAAGAAGGTCCAGTATTAAAAGAACCCGCTATTACGAGACTTACATTAGGTCTGAGTCA
  GGAGATGAGCAATTTCCAACCATTTGCCGTGAAGATCCTGAGATACACGGCTACTTCCGAGACCCCCGCT
  GCTTGGGGGAACAGGAGTATTTCAGTAGCGAGGAGTGCTGTGAGGACGACAGCTCTCCCACCTGGAGCAG
  GCAAAACTACAACTACTACAACAGGTACCCAGGCAGCTCCATGGACTTTGAGAGGCCCCGAGGCTACCAC
  CACCCCCAAGGCTTCTTAGAAGATGATGACTCTCCCACTGGCTATGATTCACGGAGATCTCCGAGGAGAC
  GTCTTCTACCTCCTACCCCACCATCCCATCGGAGGTCCTCCTTCAACTTTGAGTGTCTGCGCAGGCAAAG
  CAGCCAAGACGATGTCCTTCCATCGCCTGCCCTGCCTCACCGCGCTGCCCTGCCCCTGCACCTGATGCAG
  CAGCAGATCATGGCCGTTGCAGGCCTAGATTCCAGTAAAGCCCAGAAGTACTCACCGAGCCACTCTACCC
  GGTCGTGGGCCACCCCTCCAGCAACCCCTCCATACCGAGACTGGAGCCCATGCTACACCCCTTTGATCCA
  GGTGGACCGCTCAGAATCTATGGACCAGGTCAATGGCAGCCTGCCATCCCTACACCGAAGCTCCTGGTAC
  ACAGATGAACCCGACATCTCCTATAGGACTTTCACGCCAGCTAGTCTGACTGTCCCCAGCAGCTTCCGGA
  ACAAGAACAGCGACAAGCAGAGGAGTGCAGACAGCCTGGTGGAGGCAGTCCTGATATCCGAAGGCTTGGG
  ACGCTACGCGAGGGACCCCAAATTTGTCTCAGCGACAAAACATGAGATTGCCGATGCCTGTGACCTAACC
  ATAGACGAGATGGAAAGTGCTGCCAGCACCCTGCTCAATGGCAGTGTGTGTCCTCGAGCCAATGGGGACA
  TGGGCCCCATCTCCCACCGCCAGGACTATGAGCTCCAGGACTTTGGTCCTGGCTACAGTGACGAGGAGCC
  AGACCCTGGGCGAGAGGAAGAGGACCTGGCAGATGAGATGATTTGCATTACCACCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222925 representing NM_028981
  Red=Cloning site Green=Tags(s)
  
  MNLPTFSSDLILIKSVLSQETDARYKGRVVSAVESTEDFSQAFAEANYARGTRLPISGEGPTSQPNSSKQ
  TVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRA
  CISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLH
  PNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQ
  VVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTA
  NGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVL
  NLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPT
  SETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTL
  TISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELE
  LMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNF
  DETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFL
  AIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPY
  PPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIF
  TNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLL
  DMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFM
  FACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVS
  TFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCEL
  DKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMD
  ILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRV
  MRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFP
  QAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVI
  MDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVA
  MNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTV
  GKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPE
  EEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHN
  SIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSES
  GDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYH
  HPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQ
  QQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWY
  TDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLT
  IDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028981
• ORF Size: 6498 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028981.3, NP_083257.2
• RefSeq Size: 8705 bp
• RefSeq ORF: 6501 bp
• Locus ID: 12289
• UniProt ID: Q99246
• Cytogenetics: 14 18.43 cM
• MW: 245.7 kDa
• Gene Summary: This gene encodes a pore-forming subunit of the L-type, voltage-activated calcium channel family. These channels have been found to play a role in heart and smooth muscle contraction and in the transmission of auditory information. Homozygous knockout mice for this gene exhibit deafness and heart defects. These channels have also been linked to mitochondrial oxidative stress in a mouse model of Parkinson's disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]