S1pr4 (NM_010102) Mouse Tagged ORF Clone

CAT#: MR222799

S1pr4 (Myc-DDK-tagged) - Mouse sphingosine-1-phosphate receptor 4 (S1pr4)

Product Images

Circular map for MR222799

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: S1pr4
• Synonyms: Edg6; lp; Lpc1; S1; S1p4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222799 representing NM_010102
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACATCAGTACCTGGTCCACGCTGGTGACCCCAGAGTCCTGCCACCGGCTGGCAGCCAGCGGCCACA
  GCCTCCTCATTGTCCTGCACTACAATCACAGCGGCAGGCTGGCCAGCCGCGGGGGCTCTGAGGACGGTGG
  AGGGCTAGGGATGCTGAGGGGGCCGTCGGTGGCCGCAGGTTGCCTGGTGGTGCTGGAGAACGCCATGGTG
  CTGGCCGCCATCGCCATCTACATGCGGTCCCGCCGCTGGGTGTACTACTGCCTGCTGAACATCACACTGA
  GTGACCTGCTCACAGGCCTGGCCTACGTGGTCAACGTGCTGCTGTCAGGGACTCGTACCTTCCAGCTGTC
  ACCGGTGCACTGGTTCCTGCGGGAGGGCCTGCTCTTCATGGCCTTGGCCGCATCCACCTTCAGTCTGCTC
  TTCACGGCCGGCGAGCGCTTCGCCACCATGGTGCGGGTGGCTGAGAGTGGGGCCACCAAGACCAGCCGTG
  TGTATGGCTGCATCGGTCTGTGCTGGCTACTGGCAGCTATCCTGGGCCTGCTGCCCCTGCTGGGCTGGAA
  CTGTGTGTGCGCCTTCCCACGCTGCTCCAGCCTGCTGCCCCTCTACTCCAAGGGCTATGTGCTCTTTTGT
  GTGGTGGTCTTCGCCCTCATCCTAGTGGCTATCCTGAGCCTCTACGGGGCCATCTTTAGAGTGGTCCGAG
  CCAATGGGCAGAAGTCTCCACGTCCTCCTGCCCGCCGCAAGTCCCGCAGGCTACTCAACACCGTGCTGAT
  GATCTTGGTGGCCTTTGTGGTGTGCTGGGGTCCCCTGTTTGGCCTGCTCCTGGCTGACATCTTTGGTTCT
  AATGTCTGGGCCCAGGAGTACCTGCGTGGCATGGACTGGATCCTGGCCCTGGCCGTGTTCAACTCAGCCA
  TTAATCCTCTCATCTACTCCTTCCGCAGCCGTGAGGTGCAGCGCGCTGTGCTGGCCTTCCTGTGCTGCGG
  CTGTCTCTGGCTAGGTCTGCGAGGTCCAGGAGACTGCCTGACCCGGATCACCGAGGCCCACTCCGGTGCA
  TCCACCACTGACAGCTCCCTGAGGCCCAGGGACAGTTTTCGGACTTCTCGGTCACTCAGCTTCAGGATGA
  GAGAGCCGCTGTCCAGCATTTCCAGCGTCCGCAGCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222799 representing NM_010102
  Red=Cloning site Green=Tags(s)
  
  MNISTWSTLVTPESCHRLAASGHSLLIVLHYNHSGRLASRGGSEDGGGLGMLRGPSVAAGCLVVLENAMV
  LAAIAIYMRSRRWVYYCLLNITLSDLLTGLAYVVNVLLSGTRTFQLSPVHWFLREGLLFMALAASTFSLL
  FTAGERFATMVRVAESGATKTSRVYGCIGLCWLLAAILGLLPLLGWNCVCAFPRCSSLLPLYSKGYVLFC
  VVVFALILVAILSLYGAIFRVVRANGQKSPRPPARRKSRRLLNTVLMILVAFVVCWGPLFGLLLADIFGS
  NVWAQEYLRGMDWILALAVFNSAINPLIYSFRSREVQRAVLAFLCCGCLWLGLRGPGDCLTRITEAHSGA
  STTDSSLRPRDSFRTSRSLSFRMREPLSSISSVRST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010102
• ORF Size: 1158 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010102.2, NP_034232.1
• RefSeq Size: 2393 bp
• RefSeq ORF: 1161 bp
• Locus ID: 13611
• UniProt ID: Q9Z0L1
• Cytogenetics: 10 C1
• MW: 42.7 kDa
• Gene Summary: This gene encodes a member of the G-protein coupled receptor 1 family. The encoded protein is a receptor for the lysophospholipid sphingosine 1-phosphate. The encoded protein is one of the predominant sphingosine 1-phosphate G-protein coupled receptors expressed in T cells, and it plays a role in suppression of T cell proliferation, generation of cytokines, and potentially other aspects of immune activation. [provided by RefSeq, Feb 2010]