Zscan4d (NM_001100186) Mouse Tagged ORF Clone

CAT#: MR222789

Zscan4d (Myc-DDK-tagged) - Mouse zinc finger and SCAN domain containing 4D (Zscan4d)

Product Images

Circular map for MR222789

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zscan4d
• Synonyms: EG545913
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222789 representing NM_001100186
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCACAGCAGGCACCAGCAAAAGACCTTCAGACCAACAATTTAGAGTTTACTCCATCTCATAGTT
  CTGGTGTGCAGTGGGTAGAAGACATCTCTAACTCACCAAGTGCTCAGCTAAACTTTTCTCCAAGTAACAA
  TGGCTGCTGGGCAACTCAGGAGCTGCAAAGTCTCTGGAAGATGTTCAACTCCTGGTTGCAGCCAGAAAAG
  CAGACTAAGGAGCAGATGATTTCTCAACTGGTCTTGGAGCAGTTTCTCCTCATTGGGCACTGCAAGGACA
  AGTATGCTTTGACAGAGAAGTGGAAAGCCAGTGGTAGCGATATGAGGAGATTCATGGAGAGTCTGACTGA
  TGAGTGCTTGAAGCCTCCTGTCATGGTCCATGTTTCAATGCAAGGACAAGAAGCTCTCTTTTCTGAAAAC
  ATGCCATTAAAAGAAGTCATCAAGCTTTTGAAACAACAGCAATCTGCAACAAGGCCAACACCAGATAATG
  AGCAGATGCCAGTAGACACCACACAAGATAGATTATTGGCCACAGGACAAGAAAACAGTGAAAATGAATG
  CAACAACTCTTGTAATGCTACTGAAGCAAATGTTGGTGAAAGCTGTAGTGGAAATGAAATGGACTCCCTT
  CTTATTATCCAGAAAGAACAGTACCCTGAGCATGAAGAGGGGAATGTTGTTTTTCAATTCCCTCTTGATG
  CCAGAAGAGCAAGTCAAGGCAACTCCAGTCATCATGTAGACTTCCGGAGTGCTCCGACTCCTGCGGATGT
  CCCCATGGAGGAACAACCAAAGGATTTATCCAGAGAAAACATCTCTGAGGACAAGAACAATTGCTATAAC
  ACTTCCAGGAATGCAGCTACTCAAGTATATAGAAGTGATAATATTCCCAGGAAAAAGACAGACTCCCTTT
  CCATTAACAAGAGAATATATCATTCTGAGCCTGAGGAGGGAGATATTCCTTATGGAGTTCCTCAGGATTC
  TACAAGAGCAAGTCAAGGAACATCTACATGCTTGCAAGAGTCACTTGGGGAATGTTTTTCTGAAAAAGAC
  CCTAGGGAGCTACCAGGGTTGGAGTCTAGGCAAGAGGAGCCTATCTCTGATCCTGTCTTTCTTGGTAAGG
  ATCATGAGGCAAACTTACCATGTGAAAGTCATCAAAAGAGATTCCGTAGAGATGCCAAACTATTCAAGTG
  TGAAGAATGTTCTAGGATGTTCAAACATGCCAGGAGCCTTTCGTCCCACCAGAGAACTCACCTGAATAAG
  AAGAGTGAATTGCTTTGTGTCACCTGTCAGAAAATGTTCAAACGAGTCTCTGACCGCCGAACCCATGAGA
  TCATACACATGCCAGAAAAGCCTTTCAAGTGCAGCACATGTGAAAAGTCCTTCAGCCACAAGACCAACCT
  GAAGTCTCATGAGATGATTCACACAGGAGAAATGCCTTATGTCTGTTCCCTATGTAGCCGTCGCTTTCGC
  CAATCATCCACTTACCATCGTCACCTGAGGAATTACCACAGATCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222789 representing NM_001100186
  Red=Cloning site Green=Tags(s)
  
  MASQQAPAKDLQTNNLEFTPSHSSGVQWVEDISNSPSAQLNFSPSNNGCWATQELQSLWKMFNSWLQPEK
  QTKEQMISQLVLEQFLLIGHCKDKYALTEKWKASGSDMRRFMESLTDECLKPPVMVHVSMQGQEALFSEN
  MPLKEVIKLLKQQQSATRPTPDNEQMPVDTTQDRLLATGQENSENECNNSCNATEANVGESCSGNEMDSL
  LIIQKEQYPEHEEGNVVFQFPLDARRASQGNSSHHVDFRSAPTPADVPMEEQPKDLSRENISEDKNNCYN
  TSRNAATQVYRSDNIPRKKTDSLSINKRIYHSEPEEGDIPYGVPQDSTRASQGTSTCLQESLGECFSEKD
  PRELPGLESRQEEPISDPVFLGKDHEANLPCESHQKRFRRDAKLFKCEECSRMFKHARSLSSHQRTHLNK
  KSELLCVTCQKMFKRVSDRRTHEIIHMPEKPFKCSTCEKSFSHKTNLKSHEMIHTGEMPYVCSLCSRRFR
  QSSTYHRHLRNYHRSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100186
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100186.1, NP_001093656.1
• RefSeq Size: 1992 bp
• RefSeq ORF: 1521 bp
• Locus ID: 545913
• UniProt ID: A7KBS4
• Cytogenetics: 7 A1
• MW: 58 kDa
• Gene Summary: Transcription factor required to regulate early development. Binds telomeres and plays a key role in genomic stability by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation (By similarity).[UniProtKB/Swiss-Prot Function]