Wwp1 (NM_177327) Mouse Tagged ORF Clone

CAT#: MR222724

Wwp1 (Myc-DDK-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 1 (Wwp1)

Product Images

Circular map for MR222724

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wwp1
• Synonyms: 8030445B08Rik; AIP5; SDRP1; Tiul1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222724 representing NM_177327
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACTGCTTCACCAAGATCTGACACTAGTGATATCCACAGTGGAAGGTTACAGTTAAAGGTAACGG
  TTTCTAGTGCCAAACTAAAAAGAAAAAAGAACTGGTTTGGAACAGCAATATATACTGAAGTCATTGTAGA
  TGGAGAAGTTAAGAAAACAGCAAAATCCAGTAGTTCCTCTAATCCAAAATGGGACGAGCAGCTGATTGTA
  AATGTGACCCCACAGACCACCTTGGAGTTCCGAGTTTGGAGTCACCACACTTTAAAGGCAGATGCTCTAT
  TAGGAAAAGCAACAGTGGACTTGAAACAAGTGCTGTTAACACACAACAGAAAATTAGAAAAAGTGAAAGA
  ACAATTAAAACTTTCCTTGGAAAACAAGAATGGAATAGTGCAAACTGGGGAACTGACAGTTGTCCTTGAT
  GGATTAGTGATTGAGCAAGAGCCTGTAACAAACCGCAGCTCCTCGCCACCCATAGAAATTCAGCAAAATG
  GAGATGCCTTACATGAGAATGGAGACCCTGCAACAAGGACAACTCCCAGGTTGCCTGTTGAAGGTACTAT
  TGGAATTGATAATCATGTATCAACCAATACTGTTGTTCCCAACTCCTGCTGTTCACATGTAGTTAATGGA
  GAAAACACACCTTCATCCCCGTCTCAGGTTGCTGCCAGACCCAAAAATGCTCCAGCTCCAAAGCCAGTCA
  CATCTGCGCCCACCAGTGACACGGTTAATGGAGAATCATCATCTGTCCTAGCTGATAATACTTCAACCAT
  GGGTACTTTACTACCATCTGAAGATACCACCTCGACTTCCAATTGCACTAGCACCACCACCCAAGAGCCT
  CCTGTACAAGAACCCCCAGCATCCTCAGAACACAGTGAATGTATTCCCTCTGCCAGTGCGGAAGTGGGAC
  CAGAAGCTCGGAGCCTCATAGACCCTGACTCTGATTCTAGAAATAATTCTGTGTTTGACAAAGTAAGGCA
  GCCAGAAGGGTGTGTAGAACCTCTGCGGCCACAGTCTGGAAACACCAACACAGAAGCTTTGCCATCAGGG
  TGGGAACAGAGAAAAGATCCTCATGGTAGAACCTATTATGTGGATCATAATACTCGAACTACTACATGGG
  AGAGACCACAGCCTTTGCCTCCAGGTTGGGAAAGAAGAGTTGATGATCGTGGAAGAGTTTATTATGTGGA
  TCATAACACCAGAACAACAACCTGGCAGCGGCCTACTATGGAATCTGTTCGAAATTTTGAACAGTGGCAG
  TCTCAGCGGAATCAATTGCAGGGAGCTATGCAACAATTCAACCAACGATACCTCTATTCGGCTTCCATGT
  TAGCTGCAGAAAATGACCCCTATGGACCCTTACCACCGGGCTGGGAAAAAAGAGTGGATTCAACAGACAG
  AGTTTACTTTGTGAATCACAATACAAAAACAACCCAGTGGGAAGATCCAAGAACTCAAGGCTTACCAAAT
  GAAGAACCCCTGCCCGAAGGCTGGGAAATCAGGTATACTCGGGAAGGTGTTAGGTACTTTGTTGATCATA
  ACACGAGAACAACAACATTCAAAGACCCTCGCAATGGGAAGTCATCAGTAACTAAAGGTGGTCCACAGAT
  TGCTTATGAACGCAGCTTTAGGTGGAAGCTTGCTCACTTCCGTTACTTGTGCCAGTCTAATGCACTACCG
  AGTCATGTAAAGATCAATGTGTCCCGACAGACATTATTTGAAGATTCCTTCCAACAGATTATGGCATTAA
  AACCATATGACTTGAGGAGGCGATTATATGTAATATTTAGAGGAGAAGAAGGTCTTGATTATGGTGGCCT
  TGCAAGAGAATGGTTTTTCTTGCTTTCCCATGAAGTTTTGAACCCAATGTATTGCTTATTTGAGTATGCA
  GGCAAGAATAACTATTGTTTACAGATAAATCCAGCATCAACTATAAATCCTGACCATCTTTCATACTTCT
  GTTTTATTGGTCGCTTTATTGCAATGGCACTGTTCCATGGAAAGTTTATTGATACTGGTTTCTCTTTACC
  ATTCTACAAGCGTATGCTAAGTAAAAAACTTACTATTAAGGATTTGGAATCTATTGATACTGAATTTTAC
  AACTCTCTAATCTGGATAAGAGATAACAATATTGAAGAATGTGGCTTAGAAATGTACTTTTCTGTAGACA
  TGGAGATTCTGGGAAAAGTTACTTCACATGATTTAAAGTTGGGAGGTTCCAATATCCTGGTGACGGAAGA
  AAACAAAGATGAATATATTGGTTTAATGACAGAATGGCGTTTTTCTCGAGGAGTACAAGAACAGACCAAA
  GCTTTCCTTGATGGGTTTAATGAAGTTGTTCCCCTTCAGTGGCTACAGTACTTTGATGAAAAAGAATTGG
  AGGTTATGCTGTGTGGCATGCAAGAGGTTGACTTGGCAGACTGGCAGAGAAATACTGTTTATCGACATTA
  TACAAGAAACAGCAAGCAAATTATTTGGTTTTGGCAGTTTGTGAAAGAGACTGATAATGAAGTACGAATG
  CGACTCTTGCAGTTTGTGACTGGAACCTGTCGCTTGCCTCTAGGAGGATTTGCTGAGCTCATGGGAAGTA
  ATGGGCCTCAAAAGTTCTGTATTGAAAAAGTTGGCAAAGACACCTGGTTACCAAGAAGCCATACATGTTT
  TAATCGCTTGGATCTACCACCATATAAGAGTTATGAACAACTAAAGGAAAAACTTCTTTTTGCAATAGAA
  GAGACAGAGGGATTTGGACAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222724 representing NM_177327
  Red=Cloning site Green=Tags(s)
  
  MATASPRSDTSDIHSGRLQLKVTVSSAKLKRKKNWFGTAIYTEVIVDGEVKKTAKSSSSSNPKWDEQLIV
  NVTPQTTLEFRVWSHHTLKADALLGKATVDLKQVLLTHNRKLEKVKEQLKLSLENKNGIVQTGELTVVLD
  GLVIEQEPVTNRSSSPPIEIQQNGDALHENGDPATRTTPRLPVEGTIGIDNHVSTNTVVPNSCCSHVVNG
  ENTPSSPSQVAARPKNAPAPKPVTSAPTSDTVNGESSSVLADNTSTMGTLLPSEDTTSTSNCTSTTTQEP
  PVQEPPASSEHSECIPSASAEVGPEARSLIDPDSDSRNNSVFDKVRQPEGCVEPLRPQSGNTNTEALPSG
  WEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQ
  SQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLPN
  EEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALP
  SHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYA
  GKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFY
  NSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTK
  AFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRM
  RLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIE
  ETEGFGQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177327
• ORF Size: 2754 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177327.5, NP_796301.2
• RefSeq Size: 6458 bp
• RefSeq ORF: 2757 bp
• Locus ID: 107568
• UniProt ID: Q8BZZ3
• Cytogenetics: 4 A3
• MW: 104.7 kDa
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF (By similarity). Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7.[UniProtKB/Swiss-Prot Function]