Jak3 (NM_001190830) Mouse Tagged ORF Clone

CAT#: MR222644

Jak3 (Myc-DDK-tagged) - Mouse Janus kinase 3 (Jak3), transcript variant 2

Product Images

Circular map for MR222644

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Jak3
• Synonyms: fae; wil
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222644 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCTCCAAGTGAGGAGACACCTCTGATCCCTCAGCGCTCTTGCAGCCTCTCATCCTCAGAGGCAG
  GAGCCCTGCATGTGCTCCTTCCTCCCCGGGGACCTGGGCCTCCCCAGCGATTGTCATTCTCTTTTGGGGA
  CTACTTGGCTGAGGATTTATGTGTGCGAGCTGCCAAGGCCTGTGGCATCCTGCCTGTTTATCATTCGCTT
  TTCGCTCTGGCCACTGAGGACTTCTCTTGCTGGTTTCCCCCAAGCCACATCTTCTGCATAGAGGACGTGG
  ACACTCAAGTCTTGGTCTACAGGCTACGCTTTTATTTCCCTGACTGGTTTGGGCTGGAGACATGTCACCG
  CTTTGGGCTGCGCAAAGATTTGACCAGTGCCATCCTTGACTTACATGTTTTAGAACATCTCTTTGCTCAG
  CACCGCAGTGACCTGGTGAGTGGGCGCCTCCCGGTGGGCCTTAGCATGAAGGAGCAGGGAGAGTTCCTGA
  GCCTGGCCGTGCTGGACTTGGCCCAGATGGCTCGTGAGCAGGCCCAGCGCCCAGGAGAGCTGCTGAAGAC
  GGTCAGTTACAAAGCCTGTCTGCCGCCCAGCCTGCGCGATGTGATCCAGGGCCAGAACTTCGTGACACGC
  AGGCGCATCCGCAGGACCGTGGTCTTGGCGCTGCGCCGTGTGGTCGCCTGCCAGGCCGACCGCTACGCGC
  TCATGGCCAAGTATATTCTGGACCTGGAGCGGCTACATCCAGCGGCCACCACCGAGACCTTCCGTGTGGG
  GCTCCCGGGCGCCCAGGAGGAGCCGGGGCTTCTGCGTGTGGCGGGGGACAACGGCATCTCCTGGAGCTCC
  GGGGACCAGGAGCTTTTCCAGACCTTCTGTGACTTTCCGGAAATCGTGGATGTCAGCATCAAGCAGGCCC
  CACGTGTGGGTCCGGCAGGGGAGCACCGGCTGGTCACTGTCACCAGGATGGACGGCCACATCCTGGAAGC
  GGAGTTTCCGGGGCTGCCTGAGGCGCTGTCTTTCGTGGCCCTCGTGGATGGGTACTTCCGCCTGATCTGC
  GACTCCAGGCATTATTTCTGCAAGGAGGTGGCGCCGCCACGGCTGCTGGAGGAGGAGGCGGAGCTGTGCC
  ATGGACCCATCACGTTAGACTTTGCCATCCACAAGCTGAAGGCCGCTGGCTCCCTCCCAGGCACCTATAT
  TCTCCGCCGCAGCCCGCAGGACTATGACAGCTTTCTTCTTACCGCCTGCGTCCAGACTCCTCTTGGCCCC
  GACTACAAGGGCTGCCTCATCCGCCAGGACCCCAGCGGGGCTTTCTCCCTGGTTGGCCTCAGCCAGCCCC
  ACAGAAGCCTGCGGGAGCTGCTTGCAGCCTGCTGGAATTCTGGGCTGCGAGTAGACGGTGCTGCCCTGAA
  CCTAACATCCTGCTGCGCTCCCAGACCCAAGGAAAAGTCCAATTTGATCGTGGTGCGAAGGGGCTGCACC
  CCCGCGCCTGCCCCTGGCTGCTCCCCGTCCTGCTGTGCGCTGACACAGCTGAGCTTCCACACAATTCCAA
  CGGACAGCCTGGAGTGGCACGAGAACCTGGGTCACGGTTCTTTTACCAAGATCTTCCGTGGCCGCAGGCG
  GGAGGTCGTGGATGGTGAGACACATGACTCGGAAGTCCTCCTGAAGGTCATGGACTCCAGACATCGGAAC
  TGCATGGAGTCTTTTCTGGAAGCCGCAAGCTTGATGAGCCAAGTATCCTACCCGCACCTGGTGTTACTGC
  ACGGCGTCTGCATGGCTGGAGACAGCATCATGGTGCAGGAATTTGTGTATCTAGGAGCAATTGACATGTA
  CCTGCGCAAGCGTGGCCACCTGGTGTCAGCCAGCTGGAAACTGCAGGTGACCAAGCAGCTGGCATATGCC
  CTTAACTACTTGGAGGACAAAGGCCTTCCTCACGGCAACGTCTCAGCACGGAAGGTGCTCCTGGCTCGTG
  AGGGGGGTGATGGGAATCCACCTTTCATTAAGCTGAGTGATCCTGGTGTCAGTCCCACTGTGCTGAGCCT
  GGAAATGCTCACCGACAGAATACCCTGGGTGGCCCCCGAATGTCTCCAGGAGGCTCAGACACTCTGCTTG
  GAGGCTGACAAGTGGGGCTTTGGAGCCACCACGTGGGAGGTGTTCAGCGGGGGACCCGCCCACATCACCT
  CGCTGGAGCCCGCCAAAAAGCTGAAGTTCTATGAGGACCAGGGACAGCTGCCCGCTCTCAAATGGACAGA
  ACTGGCGGGACTTATCACACAGTGCATGGCGTATGATCCTGGCCGGCGCCCCTCCTTCCGAGCTATCCTC
  AGAGACCTCAACGGCCTCATTACATCAGATTACGAGCTCCTCTCAGACCCCACACCTGGCATCCCGAGTC
  CTCGAGATGAGCTGTGCGGTGGCGCCCAGCTCTATGCCTGCCAGGACCCCGCCATATTCGAGGAGAGACA
  CCTTAAGTACATCTCTTTGCTGGGCAATGGCAACTTTGGCAGCGTGGAGCTGTGCCGCTATGACCCCCTG
  GGGGACAATACGGGACCCCTGGTGGCAGTGAAACAGCTACAGCACAGCGGGCCAGACCAGCAGAGGGACT
  TCCAGCGGGAGATTCAGATCCTTAAGGCTCTGCACAGCGACTTCATCGTCAAGTACCGGGGAGTCAGCTA
  TGGGCCAGGTCGCCAGAGCCTGCGGTTGGTGATGGAGTACCTGCCCAGCGGCTGCCTGCGAGACTTCCTG
  CAGCGCCATCGCGCGCGCCTGCACACCGACCGCCTACTGCTGTTCGCTTGGCAGATCTGCAAGGGCATGG
  AGTACCTGGGTGCGCGCCGCTGCGTACACCGTGACCTGGCTGCGCGCAACATCTTGGTGGAGAGCGAGGC
  TCATGTGAAGATCGCGGACTTCGGCCTCGCTAAGCTGCTGCCCCTGGGAAAGGACTACTACGTGGTCCGC
  GAGCCTGGCCAAAGCCCCATCTTTTGGTATGCCCCGGAGTCCCTATCTGACAACATCTTCTCCCGCCAAT
  CTGACGTGTGGAGCTTCGGAGTGGTGTTGTACGAGCTCTTCACCTACTGCGACAAGAGCTGCAGCCCATC
  CGCTGAGTTCCTGCGCATGATGGGGCCTGAGCGTGAAGGACCCCCGCTCTGCCGCCTCCTGGAGCTGCTG
  GCAGAGGGCCGACGCCTCCCACCACCTCCCACCTGCCCCACCGAGGTTCAGGAGCTCATGCAGCTGTGCT
  GGGCGCCCAGCCCGCACGACCGGCCAGCCTTCGGCACCCTGAGCCCCCAGCTGGACGCGCTGTGGCGTGG
  AAGACCCGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222644 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPPSEETPLIPQRSCSLSSSEAGALHVLLPPRGPGPPQRLSFSFGDYLAEDLCVRAAKACGILPVYHSL
  FALATEDFSCWFPPSHIFCIEDVDTQVLVYRLRFYFPDWFGLETCHRFGLRKDLTSAILDLHVLEHLFAQ
  HRSDLVSGRLPVGLSMKEQGEFLSLAVLDLAQMAREQAQRPGELLKTVSYKACLPPSLRDVIQGQNFVTR
  RRIRRTVVLALRRVVACQADRYALMAKYILDLERLHPAATTETFRVGLPGAQEEPGLLRVAGDNGISWSS
  GDQELFQTFCDFPEIVDVSIKQAPRVGPAGEHRLVTVTRMDGHILEAEFPGLPEALSFVALVDGYFRLIC
  DSRHYFCKEVAPPRLLEEEAELCHGPITLDFAIHKLKAAGSLPGTYILRRSPQDYDSFLLTACVQTPLGP
  DYKGCLIRQDPSGAFSLVGLSQPHRSLRELLAACWNSGLRVDGAALNLTSCCAPRPKEKSNLIVVRRGCT
  PAPAPGCSPSCCALTQLSFHTIPTDSLEWHENLGHGSFTKIFRGRRREVVDGETHDSEVLLKVMDSRHRN
  CMESFLEAASLMSQVSYPHLVLLHGVCMAGDSIMVQEFVYLGAIDMYLRKRGHLVSASWKLQVTKQLAYA
  LNYLEDKGLPHGNVSARKVLLAREGGDGNPPFIKLSDPGVSPTVLSLEMLTDRIPWVAPECLQEAQTLCL
  EADKWGFGATTWEVFSGGPAHITSLEPAKKLKFYEDQGQLPALKWTELAGLITQCMAYDPGRRPSFRAIL
  RDLNGLITSDYELLSDPTPGIPSPRDELCGGAQLYACQDPAIFEERHLKYISLLGNGNFGSVELCRYDPL
  GDNTGPLVAVKQLQHSGPDQQRDFQREIQILKALHSDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFL
  QRHRARLHTDRLLLFAWQICKGMEYLGARRCVHRDLAARNILVESEAHVKIADFGLAKLLPLGKDYYVVR
  EPGQSPIFWYAPESLSDNIFSRQSDVWSFGVVLYELFTYCDKSCSPSAEFLRMMGPEREGPPLCRLLELL
  AEGRRLPPPPTCPTEVQELMQLCWAPSPHDRPAFGTLSPQLDALWRGRPG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190830
• ORF Size: 3303 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3679 bp
• RefSeq ORF: 3303 bp
• Locus ID: 16453
• UniProt ID: Q62137
• Cytogenetics: 8 34.43 cM
• MW: 122.6 kDa
• Gene Summary: Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.[UniProtKB/Swiss-Prot Function]