Gbp2b (NM_010259) Mouse Tagged ORF Clone

CAT#: MR222355

Gbp1 (Myc-DDK-tagged) - Mouse guanylate binding protein 1 (Gbp1)

Product Images

Circular map for MR222355

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gbp2b
• Synonyms: Gbp-1; Gbp1; Mag-1; Mpa-1; Mpa1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222355 representing NM_010259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCAGAAATCCACATGAAAGGCCCAGTGTGCCTCATCAAGAACTCCGGTGAGCAGTTGGAGGTCT
  ACCAGGAAGCACTGGACATCCTGTCTGCTATCCAAAATCCTGTGGTGGTCGTAGCAATAGTGGGCTTCTA
  CCACACAGGCAAATCCTATCTGATGAATAAGCTGGCTGGAAAGCAGAAGGGCTTCTCCCTGGGCTCCACT
  GTGCAGTCTCACACAAAGGGCATCTGGATGTGGTGCATGCCTCATCCCGAGAAGCCAGAACATACCCTGG
  TTCTGCTTGACACTGAGGGCCTGAAAGACATGCAGAAGGGTGACAACCAGAATGACTGTTGGATCTTTGC
  TTTGGCAGTCCTCCTCAGCAGCACCTTCATCTACAACAGCATAGGAACCATCAACCAGCAGGCCATGGAC
  CAGCTGCACTATGTGACAGAACTGACTGATCTCATCAAATCAAAGTCATCACCTGATCAGAGTGATGTAG
  ACAACTCAGCTAACTTTGTGGGCTTTTTTCCTATCTTTGTGTGGACTCTGAGGGATTTCTCCCTGGATCT
  GGAATTTGATGGAGAATCCATCACTCCTGATGAGTACCTGGAGACTTCACTGGCTCTGAGAAAAGGAACT
  GATGAGAACACTAAAAAATTTAATATGCCTCGCCTGTGTATCAGGAAGTTCTTCCCAAAGAGGAAGTGCT
  TCATCTTTGACAGGCCTGGAGACAGGAAGCAACTTTCCAAACTAGAGTGGATACAGGAGGACCAGCTGAA
  TAAAGAATTTGTAGAACAAGTTGCAGAATTCACCTCATACATCTTCAGCTATTCTGGTGTCAAGACTCTA
  TCTGGAGGCATCACAGTCAATGGGCCACGTCTAAAAAGCCTGGTGCAGACCTATGTCAGTGCCATCTGCA
  GTGGAGAACTACCCTGTATGGAGAACGCAGTCCTGACTTTGGCCCAGATAGAGAACTCAGCAGCAGTGCA
  AAAGGCCATCACCTACTACGAAGAACAGATGAATCAGAAGATCCACATGCCCACAGAAACCCTCCAGGAG
  CTCCTGGATCTGCACAGGACCTGTGAGAGGGAGGCCATTGAGGTCTTCATGAAGAATTCTTTCAAGGATG
  TAGACCAGAAGTTCCAGGAAGAATTAGGGGCCCAGCTGGAAGCCAAACGAGATGCCTTTGTTAAGAAGAA
  CATGGACATGTCATCTGCTCATTGCTCAGACTTACTGGAGGGCCTCTTTGCTCATCTGGAAGAAGAAGTG
  AAGCAGGGGACATTTTATAAACCAGGAGGCTACTACCTTTTTCTTCAAAGGAAACAAGAGCTGGAGAAAA
  AGTATATCCAGACTCCTGGAAAGGGACTCCAGGCTGAAGTGATGCTGAGAAAATACTTTGAATCCAAGGA
  GGATTTGGCTGATACACTTCTAAAGATGGACCAGTCACTCACAGAAAAGGAAAAGCAGATTGAAATGGAA
  CGTATAAAAGCAGAAGCCGCAGAAGCAGCAAATAGAGCATTGGCAGAAATGCAAAAGAAGCATGAGATGC
  TGATGGAACAGAAGGAACAGAGTTATCAAGAGCACATGAAACAGCTGACTGAGAAGATGGAGCAGGAACG
  GAAAGAGTTAATGGCAGAGCAACAAAGAATCATATCCCTTAAACTTCAGGAACAGGAAAGACTTCTCAAG
  CAAGGATTCCAGAATGAGAGCTTGCAACTACGTCAAGAGATAGAGAAAATCAAGAACATGCCTCCACCTC
  GATCATGCACCATACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222355 representing NM_010259
  Red=Cloning site Green=Tags(s)
  
  MASEIHMKGPVCLIKNSGEQLEVYQEALDILSAIQNPVVVVAIVGFYHTGKSYLMNKLAGKQKGFSLGST
  VQSHTKGIWMWCMPHPEKPEHTLVLLDTEGLKDMQKGDNQNDCWIFALAVLLSSTFIYNSIGTINQQAMD
  QLHYVTELTDLIKSKSSPDQSDVDNSANFVGFFPIFVWTLRDFSLDLEFDGESITPDEYLETSLALRKGT
  DENTKKFNMPRLCIRKFFPKRKCFIFDRPGDRKQLSKLEWIQEDQLNKEFVEQVAEFTSYIFSYSGVKTL
  SGGITVNGPRLKSLVQTYVSAICSGELPCMENAVLTLAQIENSAAVQKAITYYEEQMNQKIHMPTETLQE
  LLDLHRTCEREAIEVFMKNSFKDVDQKFQEELGAQLEAKRDAFVKKNMDMSSAHCSDLLEGLFAHLEEEV
  KQGTFYKPGGYYLFLQRKQELEKKYIQTPGKGLQAEVMLRKYFESKEDLADTLLKMDQSLTEKEKQIEME
  RIKAEAAEAANRALAEMQKKHEMLMEQKEQSYQEHMKQLTEKMEQERKELMAEQQRIISLKLQEQERLLK
  QGFQNESLQLRQEIEKIKNMPPPRSCTIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010259
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010259.2, NP_034389.2
• RefSeq Size: 2820 bp
• RefSeq ORF: 1770 bp
• Locus ID: 14468
• Cytogenetics: 3 66.69 cM
• MW: 67.8 kDa