Vav2 (NM_009500) Mouse Tagged ORF Clone

CAT#: MR222265

Vav2 (Myc-DDK-tagged) - Mouse vav 2 oncogene (Vav2)

Product Images

Circular map for MR222265

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vav2
• Synonyms: 2810040F13Rik; AI847175; Vav-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222265 representing NM_009500
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGTGGCGGCAATGCGGCCGCTGGCTCATCGACTGCAAGGTCCTGCCGCCCAACCACCGCGTCG
  TGTGGCCCTCGGCGGTGGTCTTCGACCTGGCGCAGGCGCTGCGCGACGGCGTCCTTCTGTGCCAGCTGCT
  GCACAACCTCTCCCCCGGTTCCATCGACCTTAAGGACATCAACTTCCGGCCGCAGATGTCCCAGTTCTTG
  TGTCTGAAGAATATTCGCACCTTCCTCAAAGTCTGCCATGACAAATTTGGACTAAGAAACAGTGAGCTGT
  TTGACCCTTTCGACCTCTTTGATGTCCGAGACTTCGGGAAGGTCATCTCTGCAGTGTCCCGTCTGTCCCT
  GCACAGCATCGCACAGAGCAAAGGGATCAGGCCATTTCCATCAGAGGAGACAGCTGAAAATGATGACGAC
  GTCTACCGGAGCCTGGAAGAGCTGGCTGACGAGCATGACCTGGGTGAGGACATCTACGACTGTGTCCCGT
  GTGAAGATGAAGGAGATGACATTTATGAGGACATCATCAAGGTGGAGGTGCAGCAGCCCATGAAAATGGG
  CATGACTGAGGACGACAAGAGAAGCTGCTGCTTGTTAGAGATTCAGGAGACCGAGGCCAAGTACTACCGC
  ACCCTGGAGGACATTGAGAAGAACTACATGGGTCCCTTGCGGCTGGTGCTGAGCCCGGCGGATATGGCTG
  CTGTCTTCATCAACCTGGAGGACCTCATCAAGGTGCATCACAGCTTTCTGCGAGCCATCGATGTGTCCAT
  GATGGCTGGTGGCAGTACCCTGGCTAAGGTCTTTCTGGAGTTTAAGGAAAGGCTCCTGATCTATGGAGAG
  TACTGTAGCCACATGGAACACGCTCAGAGTACACTGAACCAGCTCCTCGCCAGCCGAGAGGACTTCAGGC
  AGAAAGTGGAGGAGTGCACACTCAGGGTTCAGGATGGCAAGTTCAAGCTGCAAGACCTGCTGGTGGTGCC
  CATGCAACGGGTGCTGAAGTACCACCTGCTGCTCAAGGAGCTCCTGAGCCATTCTGCAGACCGACCAGAA
  AGACAACAGCTCAAAGAAGCCCTGGAAGCCATGCAGGACTTGGCCATGTACATCAATGAAGTGAAGCGGG
  ACAAGGAGACCTTGAAGAAGATTAGCGAGTTCCAGTGCTCCATAGAAAACCTGCAAGTGAAGCTGGAGGA
  ATTTGGGAGGCCAAAGATTGACGGGGAGCTTAAAGTCCGGTCCATAGTCAACCACACCAAGCAAGACAGG
  TACCTGTTCCTATTTGACAAGGTGGTCATCGTGTGCAAGAGGAAGGGCTACAGCTATGAGCTGAAGGAGG
  TCATTGAGCTGCTCTTCCACAAGATGACCGATGACCCGATGCACAACAAGGACATCAAGAAGTGGTCCTA
  TGGCTTCTACCTGATTCACCTCCAAGGAAAGCAAGGCTTTCAGTTCTTCTGCAAGACGGAAGACATGAAG
  CGGAAGTGGATGGAGCAGTTCGAGATGGCCATGTCAAACATCAAGCCAGATAAGGCCAATGCCAACCATC
  ATAGCTTCCAGATGTACACATTCGACAAGACTACCAACTGCAAAGCCTGCAAGATGTTTCTCAGGGGTAC
  CTTCTACCAGGGATACCTGTGTACCAGATGTGGCGTCGGGGCACACAAGGAATGCCTGGAGGTGATCCCC
  CCCTGCAAGATGAGTTCACCTGCAGATGTGGATGCTCCTGGAGCAGGACCAGGTCCCAAGATGGTTGCTG
  TGCAGAATTACCATGGTAACCCAGCCCCTCCTGGGAAGCCTGTGTTGACCTTCCAGACAGGAGACGTGAT
  AGAACTGCTCCGGGGTGACCCCGATTCTCCTTGGTGGGAAGGGCGGCTGGTTCAAACCAGGAAGTCAGGG
  TATTTTCCCAGCTCATCTGTGAAGCCCTGCCCAGTGGATGGAAGGCCGCCTACTGGCCGCCCACCATCCC
  GGGAGATCGATTACACTGCCTACCCGTGGTTTGCTGGCAACATGGAACGGCAGCAGACGGACAATCTGCT
  CAAGTCTCATGCCAGTGGGACCTACCTCATCAGAGAGCGTCCAGCAGAGGCAGAACGCTTCGCCATCAGC
  ATCAAGTTCAATGATGAGGTGAAACATATCAAGGTGGTGGAGAAAGATAGCTGGATCCACATCACGGAAG
  CTAAGAAGTTTGAGAGCCTCCTGGAGCTGGTGGAGTACTACCAGTGTCACTCACTCAAGGAAAGCTTCAA
  GCAGTTGGACACTACACTCAAGTTCCCCTACAAGTCTCGGGAGCGAACCACCAGCAGGGCCTCAAGCCGG
  TCTCCAGCTTCCTGCGCTTCCTACAACTTTTCTTTTCTCAGTCCTCAGGGCCTCAGCTTTGCCCCCCAGG
  CCCCTTCTGCTCCCTTCTGGTCAGTGTTCACACCGCGGGTCATCGGCACAGCCGTGGCCAGGTACAACTT
  TGCTGCTCGAGACATGCGGGAGCTGTCACTGCGAGAAGGTGATGTGGTGAAGATCTACAGCCGAATTGGT
  GGGGACCAGGGCTGGTGGAAAGGAGAGACAAATGGGCGGATCGGTTGGTTTCCTTCGACGTACGTGGAAG
  AGGAAGGCGTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222265 representing NM_009500
  Red=Cloning site Green=Tags(s)
  
  MEQWRQCGRWLIDCKVLPPNHRVVWPSAVVFDLAQALRDGVLLCQLLHNLSPGSIDLKDINFRPQMSQFL
  CLKNIRTFLKVCHDKFGLRNSELFDPFDLFDVRDFGKVISAVSRLSLHSIAQSKGIRPFPSEETAENDDD
  VYRSLEELADEHDLGEDIYDCVPCEDEGDDIYEDIIKVEVQQPMKMGMTEDDKRSCCLLEIQETEAKYYR
  TLEDIEKNYMGPLRLVLSPADMAAVFINLEDLIKVHHSFLRAIDVSMMAGGSTLAKVFLEFKERLLIYGE
  YCSHMEHAQSTLNQLLASREDFRQKVEECTLRVQDGKFKLQDLLVVPMQRVLKYHLLLKELLSHSADRPE
  RQQLKEALEAMQDLAMYINEVKRDKETLKKISEFQCSIENLQVKLEEFGRPKIDGELKVRSIVNHTKQDR
  YLFLFDKVVIVCKRKGYSYELKEVIELLFHKMTDDPMHNKDIKKWSYGFYLIHLQGKQGFQFFCKTEDMK
  RKWMEQFEMAMSNIKPDKANANHHSFQMYTFDKTTNCKACKMFLRGTFYQGYLCTRCGVGAHKECLEVIP
  PCKMSSPADVDAPGAGPGPKMVAVQNYHGNPAPPGKPVLTFQTGDVIELLRGDPDSPWWEGRLVQTRKSG
  YFPSSSVKPCPVDGRPPTGRPPSREIDYTAYPWFAGNMERQQTDNLLKSHASGTYLIRERPAEAERFAIS
  IKFNDEVKHIKVVEKDSWIHITEAKKFESLLELVEYYQCHSLKESFKQLDTTLKFPYKSRERTTSRASSR
  SPASCASYNFSFLSPQGLSFAPQAPSAPFWSVFTPRVIGTAVARYNFAARDMRELSLREGDVVKIYSRIG
  GDQGWWKGETNGRIGWFPSTYVEEEGVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009500
• ORF Size: 2604 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009500.2, NP_033526.1
• RefSeq Size: 5812 bp
• RefSeq ORF: 2607 bp
• Locus ID: 22325
• UniProt ID: Q60992
• Cytogenetics: 2 19.36 cM
• MW: 99.9 kDa
• Gene Summary: This gene encodes a member of the Vav family of Rho guanine nucleotide exchange factors. Vav family proteins are involved in the development and activation of lymphocytes, and the encoded protein may also be involved in angiogenesis. Disruption of this gene in mice is associated with heart, artery, and kidney defects, as well as tachycardia and hypertension. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]