Fezf1 (NM_028462) Mouse Tagged ORF Clone

CAT#: MR222210

Fezf1 (Myc-DDK-tagged) - Mouse Fez family zinc finger 1 (Fezf1)

Product Images

Circular map for MR222210

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fezf1
• Synonyms: 3110069A13Rik; Fez; fez-like; Zfp312-like
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222210 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGTAGCTGCCTCAACGCGACCACCAAAATGCTAGCGACTGCTCCAGCTCGGGGCAACGTGATGA
  GCACGTCTAAACCCTTGGCTTTCTCCATCGAACGAATCATGGCGCGCACCCCTGAGCCTAAGGCCCTGCC
  GGTCCCCCACTTCCTGCAGGGAGCCGTGCCCAAAGGGGACCCCAAGCACTCTCTGCATCTCAACTCGTCG
  ATCCCCTGCATGATCCCTTTTGTCCCCGTGGCGTACGACACGAACTCCAAAGCGGGAGTGAATGGCTCAG
  AGCCCCGCAAGGCGAGTTTGGAGGTTCCGGCGCCGCCCGCGGTGGCGCCCTCTGCGCCGGCGTTCAGCTG
  CAGCGACCTGCTCAACTGCGCGCTAAGTCTTAAGGGCGACCTGGCCCGCGACGCATTGCCACTACAGCAG
  TACAAGCTGGTAAGGCCGCGTGTGGTCAACCACTCTTCGTTTCACGCCATGGGTGCCCTGTGCTACCTGA
  ACCGAGGTGACGGCCCGTGCCACCCGGCTGCGAGCGTGAACATCCACCCGGTGGCCTCCTACTTTCTCAG
  TTCCCCTTTGCACCCACAGCCCAAAACGTATTTAGCCGAAAGGAATAAACTGGTTGTACCGGCAGTGGAG
  AAACTCCCTTCGGGAGTAGCTTTCAAAGACCTGTCCCAGGCTCAGCTGCAGCATTACATGAAAGAAAGCG
  CCCAACTTTTGTCGGAAAAAATCGCTTTCAAAACCTCGGATTTCAGCCGCGGCTCTCCAAATGCCAAGCC
  CAAAGTTTTCACTTGTGAAGTCTGCGGCAAGGTCTTTAATGCGCACTATAACTTAACCCGTCACATGCCG
  GTACACACAGGAGCCAGACCCTTCGTGTGTAAAGTGTGTGGGAAAGGTTTCCGGCAAGCTAGCACCCTTT
  GCCGGCATAAGATCATTCACACACAGGAGAAACCTCACAAGTGCAACCAGTGCGGTAAAGCGTTTAACAG
  AAGTTCCACGTTAAACACACATACCCGAATTCACGCGGGCTACAAACCATTCGTGTGTGAATTCTGCGGC
  AAAGGATTTCATCAAAAAGGGAATTACAAAAATCACAAGTTGACCCACAGCGGGGAGAAACAGTTCAAGT
  GCAATATCTGCAACAAGGCTTTCCACCAGGTGTACAACCTCACCTTCCACATGCACACTCACAACGACAA
  GAAGCCTTTCACCTGCCCCACGTGTGGCAAGGGCTTTTGTAGGAACTTTGACCTCAAGAAGCACGTCCGC
  AAGCTACACGACAGCAGCCTGGGGCTGACCCGCACTCCTACTGGGGAGCCAAGCAGCGATCCGCCGCCCC
  AGCTGCAACAGCCGCCGCCTGCGCCTCTGCCGCCACTGCAGCCTACATTGCCGCCTCCCGGGCCTCTGCC
  ATCCGGGCTTCACCAAGGCCACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222210 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSSCLNATTKMLATAPARGNVMSTSKPLAFSIERIMARTPEPKALPVPHFLQGAVPKGDPKHSLHLNSS
  IPCMIPFVPVAYDTNSKAGVNGSEPRKASLEVPAPPAVAPSAPAFSCSDLLNCALSLKGDLARDALPLQQ
  YKLVRPRVVNHSSFHAMGALCYLNRGDGPCHPAASVNIHPVASYFLSSPLHPQPKTYLAERNKLVVPAVE
  KLPSGVAFKDLSQAQLQHYMKESAQLLSEKIAFKTSDFSRGSPNAKPKVFTCEVCGKVFNAHYNLTRHMP
  VHTGARPFVCKVCGKGFRQASTLCRHKIIHTQEKPHKCNQCGKAFNRSSTLNTHTRIHAGYKPFVCEFCG
  KGFHQKGNYKNHKLTHSGEKQFKCNICNKAFHQVYNLTFHMHTHNDKKPFTCPTCGKGFCRNFDLKKHVR
  KLHDSSLGLTRTPTGEPSSDPPPQLQQPPPAPLPPLQPTLPPPGPLPSGLHQGHQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028462
• ORF Size: 1428 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028462.1, NP_082738.1
• RefSeq Size: 2308 bp
• RefSeq ORF: 1428 bp
• Locus ID: 73191
• UniProt ID: Q0VDQ9
• Cytogenetics: 6 A3.1
• MW: 52 kDa
• Gene Summary: Transcription repressor. Involved in the axonal projection and proper termination of olfactory sensory neurons (OSN). Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation. Expression is required in OSN to cell-autonomously regulate OSN axon projections. Regulates non-cell-autonomously the layer formation of the olfactory bulb development and the interneurons. May be required for correct rostral migration of the interneuron progenitors.[UniProtKB/Swiss-Prot Function]