Dctn1 (NM_007835) Mouse Tagged ORF Clone

CAT#: MR222193

Dctn1 (Myc-DDK-tagged) - Mouse dynactin 1 (Dctn1), transcript variant 1

Product Images

Circular map for MR222193

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dctn1
• Synonyms: AL022633; DAP-150; DP-150; Glued; p150; p150-glued; p150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222193 representing NM_007835
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGAGCAGGAGGCACATGTCCAGTCGGACGCCGAGTGGCAGCAGGATGAGTACGGAGGCAAGCG
  CCCGGCCCCTGCGGGTTGGCTCCCGCGTGGAGGTGATTGGGAAGGGCCACCGAGGCACTGTGGCCTATGT
  TGGAGCCACACTCTTTGCCACTGGCAAATGGGTGGGCGTGATTCTGGATGAAGCAAAAGGCAAAAATGAT
  GGCACTGTCCAGGGAAGGAAGTATTTCACATGTGATGAAGGCCACGGCATCTTTGTACGCCAGTCCCAGA
  TCCAAGTATTTGAAGATGGAGCAGATACTACTTCCCCAGAGACTCCTGATTCTTCTGCTTCAAAGGTCCT
  CAAGAGAGAGGGAGCCGATGCAGCTGCAAAGACCAGCAAACTGCGGGGACTGAAGCCTAAGAAGGCACCG
  ACAGCCCGAAAGACCACAACTCGACGGCCCAAGCCTACTCGCCCAGCCAGCACTGGGGTGGCTGGGCCCA
  GTAGCTCCCTTGGCCCCTCTGGCTCAGCGTCAGCCGGGGAACTAAGCAGCAGTGAGCCCAGCACCCCAGC
  TCAGACTCCGCTGGCAGCACCCATCATCCCCACACCGGCCCTCACCTCTCCTGGAGCAGCACCCCCACTT
  CCATCTCCCTCTAAGGAAGAGGAAGGGCTGAGGGCTCAGGTACGGGACCTGGAGGAGAAGCTGGAGACCC
  TGCGCCTAAAACGCTCAGAAGACAAAGCAAAGCTGAAAGAGCTGGAGAAGCACAAGATCCAGCTGGAGCA
  GGTGCAGGAATGGAAGAGCAAAATGCAGGAGCAGCAGGCAGACCTGCAGCGGCGCCTCAAGGAGGCTCGG
  AAGGAAGCCAAGGAGGCGCTAGAGGCAAAGGAACGCTACATGGAGGAGATGGCCGACACAGCCGACGCTA
  TCGAGATGGCCACTCTGGACAAGGAGATGGCTGAAGAGCGCGCTGAGTCTCTGCAGCAAGAGGTGGAGGC
  ACTGAAGGAACGGGTAGACGAGCTCACCACAGACCTGGAGATTCTCAAGGCTGAAATCGAAGAGAAAGGC
  TCTGATGGGGCCGCATCAAGCTACCAGCTCAAGCAGCTGGAGGAGCAGAATGCCCGCCTGAAGGATGCCC
  TGGTGAGGATGCGAGACCTCTCTTCCTCAGAGAAGCAGGAGCACGTGAAGCTGCAGAAACTCATGGAAAA
  GAAAAACCAGGAGCTGGAGGTCGTGCGGCAGCAGCGCGAGCGTCTTCAGGAGGAGCTGAGCCAGGCTGAG
  AGCACCATCGATGAGCTCAAAGAGCAGGTGGACGCCGCTCTGGGAGCCGAGGAGATGGTGGAGATGCTGA
  CCGACCGGAACCTGAATCTAGAGGAGAAAGTGCGGGAGTTACGGGAGACTGTGGGGGACTTGGAAGCCAT
  GAACGAGATGAACGATGAGCTGCAGGAGAACGCACGGGAGACGGAGCTGGAACTCCGAGAGCAGCTGGAC
  ATGGCGGGCGCCCGAGTGAGGGAAGCGCAGAAGCGAGTGGAAGCCGCCCAGGAGACAGTCGCCGACTACC
  AGCAGACCATCAAGAAGTACCGCCAGTTGACTGCCCACCTACAGGATGTCAATCGGGAGCTGACAAACCA
  GCAGGAAGCGTCTGTAGAGAGGCAGCAGCAGCCGCCGCCAGAGACTTTTGATTTCAAAATCAAGTTTGCT
  GAGACCAAGGCTCATGCCAAGGCCATTGAGATGGAGTTGAGACAGATGGAAGTTGCCCAGGCCAACCGGC
  ACATGTCCCTGCTGACAGCCTTTATGCCTGACAGCTTCCTTCGGCCAGGTGGAGACCACGACTGTGTCCT
  GGTGCTGCTGCTCATGCCCCGACTCATTTGCAAGGCAGAGCTCATCCGGAAGCAGGCCCAGGAGAAGTTT
  GACCTGAGCGAGAACTGTTCGGAGCGGCCCGGGCTGCGGGGAGCTGCCGGGGAGCAGCTGAGCTTTGCTG
  CTGGACTGGTGTACTCGCTGAGTCTGCTGCAGGCCACGCTGCACCGCTATGAGCATGCCCTCTCTCAGTG
  CAGTGTGGACGTGTATAAGAAGGTCGGCAGCCTGTACCCCGAGATGAGCGCCCACGAGCGCTCCTTAGAT
  TTCCTCATTGAGCTGCTGCACAAGGATCAGCTGGATGAGACTGTCAACGTGGAGCCCCTCACCAAGGCCA
  TCAAGTATTACCAGCATCTGTACAGCATCCACCTCGCTGAACAACCCGAGGACTCCACCATGCAGCTGGC
  TGACCACATCAAGTTCACCCAGAGTGCCCTGGACTGCATGGGCGTGGAGGTGGGGCGGCTGCGTGCCTTC
  TTGCAGGGTGGGCAGGAGGCAACAGATATTGCCCTTCTTCTCCGAGACCTGGAAACATCATGTAGTGACA
  CCCGTCAGTTCTGCAAGAAGATCCGAAGGCGGATGCCGGGGACGGATGCTCCTGGGATCCCAGCAGCGCT
  GGCCTTTGGCTCACAGGTATCCGACACACTCCTGGACTGCAGGAAGCACTTGACGTGGGTGGTAGCTGTT
  CTGCAGGAGGTGGCAGCTGCAGCCGCCCAGCTTATTGCCCCCTTGGCAGAGAACGAGGGGCTGCCTGTGG
  CTGCACTGGAGGAGCTGGCCTTCAAAGCAAGCGAGCAGATCTACGGGAGCCCCTCCAGCAGCCCCTATGA
  GTGTCTACGCCAGTCATGCACCATCCTCATCAGCACGATGAACAAGCTGGCCACAGCCATGCAAGAAGGC
  GAGTATGACGCAGAGCGACCCCCGAGCAAGCCTCCTCCGGTTGAACTTCGGGCTGCAGCCCTGCGTGCAG
  AGATCACAGATGCTGAAGGTCTGGGTTTGAAGCTTGAGGATCGAGAGACAGTTATCAAGGAGTTAAAGAA
  GTCACTCAAGATTAAGGGAGAGGAGCTGAGTGAGGCCAACGTGCGGCTCAGCCTCCTGGAGAAGAAGTTG
  GACAGCGCTGCCAAGGATGCAGACGAGCGAATCGAGAAAGTTCAGACACGGCTGGACGAGACTCAGACCC
  TGCTGCGGAAGAAGGAGAAAGACTTTGAGGAGACAATGGACGCACTCCAGGCTGACATCGACCAGCTGGA
  GGCAGAGAAGGCAGAGCTCAAGCAGCGCCTGAACAGCCAGTCCAAGCGCACAATCGAGGGGCTCCGGGGC
  CCCCCTCCGTCAGGCATCGCTACCCTGGTCTCTGGCATCGCTGGTGAGGAACCACAGCGAGGGGGCGCTC
  CTGGGCAGGCTCCGGGCGCCTTGCCAGGCCCGGGGCTGGTGAAGGACTCCCCACTGCTGCTTCAGCAGAT
  CTCTGCTATGAGGCTACACATCTCTCAGCTCCAGCATGAGAACAGCATCCTCAGAGGAGCCCAGATGAAG
  GCGTCCTTGGCAGCTCTGCCCCCTCTGCATGTTGCAAAGCTTTCCCTCCCACCCCATGAGGGCCCCGGTG
  GTAACCTAGTGGCTGGGGCACTGTACCGCAAGACCAGCCAGCTCCTGGAGAAACTAAACCAGCTGAGTAC
  CCACACCCACGTGGTGGATATCACTCGGAGCAGCCCAGCTGCCAAGAGCCCGTCAGCTCAGCTTATGGAA
  CAAGTGGCTCAGCTCAAGTCCCTGAGTGACACCATTGAGAAGCTCAAGGATGAGGTCCTCAAGGAGACAG
  TGACTCAGCGCCCTGGAGCCACTGTGCCCACCGACTTTGCCACTTTCCCTTCATCTGCCTTCCTCAGGGC
  CAAGGAAGAGCAGCAAGATGACACAGTCTACATGGGCAAAGTGACCTTTTCATGCGCGGCAGGCCTAGGA
  CAGCGACACCGCCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGTCGCCTCATCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222193 representing NM_007835
  Red=Cloning site Green=Tags(s)
  
  MAQSRRHMSSRTPSGSRMSTEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKND
  GTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGADAAAKTSKLRGLKPKKAP
  TARKTTTRRPKPTRPASTGVAGPSSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPALTSPGAAPPL
  PSPSKEEEGLRAQVRDLEEKLETLRLKRSEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEAR
  KEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKG
  SDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAE
  STIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLD
  MAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFA
  ETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKF
  DLSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLD
  FLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDSTMQLADHIKFTQSALDCMGVEVGRLRAF
  LQGGQEATDIALLLRDLETSCSDTRQFCKKIRRRMPGTDAPGIPAALAFGSQVSDTLLDCRKHLTWVVAV
  LQEVAAAAAQLIAPLAENEGLPVAALEELAFKASEQIYGSPSSSPYECLRQSCTILISTMNKLATAMQEG
  EYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKL
  DSAAKDADERIEKVQTRLDETQTLLRKKEKDFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRG
  PPPSGIATLVSGIAGEEPQRGGAPGQAPGALPGPGLVKDSPLLLQQISAMRLHISQLQHENSILRGAQMK
  ASLAALPPLHVAKLSLPPHEGPGGNLVAGALYRKTSQLLEKLNQLSTHTHVVDITRSSPAAKSPSAQLME
  QVAQLKSLSDTIEKLKDEVLKETVTQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGLG
  QRHRLVLTQEQLHQLHSRLIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007835
• ORF Size: 3843 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007835.2, NP_031861.2
• RefSeq Size: 4437 bp
• RefSeq ORF: 3846 bp
• Locus ID: 13191
• UniProt ID: O08788
• Cytogenetics: 6 35.94 cM
• MW: 141.7 kDa
• Gene Summary: Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule. Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. Plays a role in metaphase spindle orientation. Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitement to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole. Plays a role in primary cilia formation.[UniProtKB/Swiss-Prot Function]