Myf6 (NM_008657) Mouse Tagged ORF Clone

CAT#: MR222107

Myf6 (Myc-DDK-tagged) - Mouse myogenic factor 6 (Myf6)

Product Images

Circular map for MR222107

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myf6
• Synonyms: bHLHc4; herculin; MRF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222107 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATGGACCTTTTTGAAACTGGCTCCTATTTCTTCTACTTAGATGGAGAAAATGTGACTCTTCAGC
  CATTAGAAGTGGCAGAGGGCTCTCCTTTGTATCCAGGGAGTGATGGTACCCTATCCCCTTGCCAGGACCA
  AATGCCCCAGGAAGCCGGGAGCGACAGCAGTGGAGAGGAACACGTTCTGGCTCCCCCAGGCCTGCAGCCT
  CCCCACTGCCCTGGCCAGTGTCTGATCTGGGCTTGCAAGACTTGCAAGAGAAAATCTGCCCCCACAGATC
  GTCGGAAAGCAGCTACCCTGCGCGAAAGGAGGAGACTAAAGAAAATCAACGAAGCCTTTGAGGCCCTGAA
  GCGTCGGACTGTGGCCAACCCCAACCAGAGACTGCCCAAGGTGGAGATTCTGCGGAGTGCCATCAGCTAC
  ATTGAGCGTCTACAGGACCTGCTGCACCGGCTGGATCAGCAAGAGAAGATGCAGGAGCTGGGCGTGGACC
  CCTACAGCTACAAACCCAAGCAAGAAATTCTTGAGGGTGCGGATTTCCTGCGCACCTGCAGCCCCCAGTG
  GCCAAGTGTTTCGGATCATTCCAGGGGCCTCGTGATAACTGCTAAGGAAGGAGGAGCAAACGTGGATGCC
  TCAGCCTCCAGCAGTCTTCAGCGCCTTTCTTCCATCGTGGACAGTATTTCTTCAGAGGAACGCAAACTCC
  CCAGCGTGGAGGAGGTGGTGGAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222107 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMMDLFETGSYFFYLDGENVTLQPLEVAEGSPLYPGSDGTLSPCQDQMPQEAGSDSSGEEHVLAPPGLQP
  PHCPGQCLIWACKTCKRKSAPTDRRKAATLRERRRLKKINEAFEALKRRTVANPNQRLPKVEILRSAISY
  IERLQDLLHRLDQQEKMQELGVDPYSYKPKQEILEGADFLRTCSPQWPSVSDHSRGLVITAKEGGANVDA
  SASSSLQRLSSIVDSISSEERKLPSVEEVVEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008657
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008657.3
• RefSeq Size: 1248 bp
• RefSeq ORF: 729 bp
• Locus ID: 17878
• UniProt ID: P15375
• Cytogenetics: 10 55.96 cM
• MW: 27 kDa
• Gene Summary: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.[UniProtKB/Swiss-Prot Function]