Tpx2 (NM_028109) Mouse Tagged ORF Clone

CAT#: MR221964

Tpx2 (Myc-DDK-tagged) - Mouse TPX2, microtubule-associated protein homolog (Xenopus laevis) (Tpx2), transcript variant 2

Product Images

Circular map for MR221964

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tpx2
• Synonyms: 2610005B21Rik; DIL2; p100; REPP86
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221964 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACAAGTCCCTACTACTTACTCTTTCGATGCCCCCACCGACTTTATCAATTTTTCATCTTTGGATG
  CTGAAGAGGATACTGAAAATATAGACTCATGGTTTGATGAGAAGGCCAACTTGGAGAACAAGTTTCTTCG
  ACAGAGGGGAATAGGCGAGCCTTTTCAGGGGAAGAATTCCTTGAGAAAAGCCAAACTTCAACAGGGCTTC
  GTCACACCGTTGAAGGCAGTTGACAACACTTACCACAAAGAGACAGAAAAGGAAAATCTTCAGAAACAGT
  CTATCCCATCAAATGATTGTTCTTCCCTGGATGCTAAGAGAGCTGTATCAGGAAATACTCCTGTCCAGCC
  TCAGAGAAGATCTATTAGACTCTCTGCTCAGAAGGATTTGGAGCAGAAAGAGAAAAACCATGTTGCCTCT
  GTTGAAATGAAAGCCAAGAGATGTGTTGCTCCGGCCACTGATTGTCCCCCCCAGAAAAGAATGAAAGTTT
  CTGATAAAAAGAAACTGGAGGAAGAGGAGGAAGGCAGTGCTCCAGCTACTTCAAGAAAGAATGAAAGAGA
  AACCCTGGAGAAAGCCAAGGGCAAGCACACTGTGCCAGGTGTGCCACCTGCAAGGGAGAAGGTTCTAAAG
  AGTACTGAGGAGCAGGAGATAGAGAAAAGGCTGCGGATGCAGCAGGAGGTGGTGGAGCTGCGCAGGAAAA
  ACGAAGAGTTCAAGAAGCTCGCGCTCGCAGGGCCAGGGCAACCTGTGAAGAAGTCCACGAGCCAGGTTAC
  CAAGACAGTTGACTTTCACTTCCTCACAGATGAGCGAATCAAGCAACATCCCAAGAACCAGGAAGAGTAT
  AAGGAAGTGAACTTCATGTCTGAACTTCGGAAGCATTCTTCCACGCCTGCCCGAGGAACCAGAGGATGCA
  CTATCATTAAGCCTTTCAACCTGTCCAAAGGGAAGAAAAGAACATTTGATGAAGCAGCTTCTACGTATGT
  GCCCATTGCACAGCAGGTTGAAGCCTTCCACAAACGAACCCCCAATAGATACCATCTGAGGAACAAGAAG
  GACGAGAGCTTGTTACCCTCCAAATCTGTGAACAAGATTGCACGAGACCCCCAGACCCCCATACTGCAGA
  CCAAATATCGTACAAGGGCTGTGACTTGCAAAAGTACTGCAGAGCAGGAGGCCGAGGAGCTTGAGAAACT
  GCAACAATACAAATTCAAAGCACGGGAACTTGATCCTAGAATTTTTGAAAGTGGCCCCATCTTGCCCAAG
  AGAGCACCTGTTAAACCTCCTACTCAGCCTGTTGGTTTTGATTTGGAAATTGAGAAACGAATTCATGAGC
  GAGAGTCAAAGAAAAAAACAGAAGATGAACAATTTGAATTTCATTCTAGACCTTGTCCTACTAAGATCTT
  GGAAGATGTCGTGGGTGTTCCTGAAAAGAAGGTAATTCCAGCTACTGTCCCGAAGTCGCCAGTTTTTGCA
  TTGAAGAACAGGATCCGAGTGCCCATCAAAGATGAGGAAGAGGAGAAACCAGTAGTGATAAAAGCTCAAC
  CTGTGCCACATTATGGGGTGCCTTATAAGCCCCACATCGCAGAGGCAAGAAATGTGGAGGTGTGCCCGTT
  CTCCTTTGATACCCGGGACAAAGAACGCCAGTTGCAGAAGGAGAAAAAAATAAAAGAGATGCAGAAAGGG
  GAGGTGCCCAAGTTCAAGGCACTTCCTGTGCCCCATTTTGACACCATTAACCTGCCAGAGAAAAAGGTAA
  AGAACGTGACTCAAGCTGAGCCTTTCTCCCTGGAGACAGACAAGAGAGGCGCCTACAAGGCTGAGATGTG
  GAAGCACCAGCTGGAGGAAGAGCAGAAGCAGCAGAAGGACGCCGCTTGCTTCAAGGCTCGTCCAAACACC
  GTCATCTTCCAAGAGCCCTTCGTTCCCAAGAAGGAGAAAAAATCACTGGCTGAGAACCCTTCTGGTTCTC
  TAGTTCAGGAACCTTTTCAGCTGGCCACCGAGAGGAGAGCCAAAGAGCGGCAGGAGCTGGAGAAGAAAAT
  GGCTGAAGTAGAGGCCTGGAAACTGCAGCAGTTGGAGGAAGTCAGGCAGCAGGAAGAGGAGCAGCAGAAG
  GAGGAGTTAGCCAGGCTCCGGAAAGAACTGGTGCACAAGGCAAATCCAATACGGAAGTACGCAGCAGTGG
  AGGTGAAATCTAGCGAGCTGCCTCTGACTGTGCCGGTGTCTCCTAAGTTCTCCACTCGGTTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221964 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQVPTTYSFDAPTDFINFSSLDAEEDTENIDSWFDEKANLENKFLRQRGIGEPFQGKNSLRKAKLQQGF
  VTPLKAVDNTYHKETEKENLQKQSIPSNDCSSLDAKRAVSGNTPVQPQRRSIRLSAQKDLEQKEKNHVAS
  VEMKAKRCVAPATDCPPQKRMKVSDKKKLEEEEEGSAPATSRKNERETLEKAKGKHTVPGVPPAREKVLK
  STEEQEIEKRLRMQQEVVELRRKNEEFKKLALAGPGQPVKKSTSQVTKTVDFHFLTDERIKQHPKNQEEY
  KEVNFMSELRKHSSTPARGTRGCTIIKPFNLSKGKKRTFDEAASTYVPIAQQVEAFHKRTPNRYHLRNKK
  DESLLPSKSVNKIARDPQTPILQTKYRTRAVTCKSTAEQEAEELEKLQQYKFKARELDPRIFESGPILPK
  RAPVKPPTQPVGFDLEIEKRIHERESKKKTEDEQFEFHSRPCPTKILEDVVGVPEKKVIPATVPKSPVFA
  LKNRIRVPIKDEEEEKPVVIKAQPVPHYGVPYKPHIAEARNVEVCPFSFDTRDKERQLQKEKKIKEMQKG
  EVPKFKALPVPHFDTINLPEKKVKNVTQAEPFSLETDKRGAYKAEMWKHQLEEEQKQQKDAACFKARPNT
  VIFQEPFVPKKEKKSLAENPSGSLVQEPFQLATERRAKERQELEKKMAEVEAWKLQQLEEVRQQEEEQQK
  EELARLRKELVHKANPIRKYAAVEVKSSELPLTVPVSPKFSTRFQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028109
• ORF Size: 2238 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028109.2
• RefSeq Size: 4352 bp
• RefSeq ORF: 2238 bp
• Locus ID: 72119
• UniProt ID: A2APB8
• Cytogenetics: 2 H1
• MW: 85.9 kDa
• Gene Summary: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.[UniProtKB/Swiss-Prot Function]