Pfkm (NM_001163488) Mouse Tagged ORF Clone

CAT#: MR221898

Pfkm (Myc-DDK-tagged) - Mouse phosphofructokinase, muscle (Pfkm), transcript variant 3

Product Images

Circular map for MR221898

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkm
• Synonyms: AI131669; ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka; Pfkx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221898 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCATGAAGAGCATCATGCAGCCAAAACCCTGGGGATCGGCAAGGCCATCGCCGTGTTGACCTCTG
  GTGGAGATGCCCAAGGTATGAATGCTGCCGTCAGGGCTGTGGTCCGAGTTGGTATCTTCACGGGCGCCCG
  CGTCTTCTTTGTCCATGAGGGTTACCAAGGCCTGGTGGACGGTGGCGAGCACATCAGGGAGGCCACGTGG
  GAGAGCGTGTCTATGATGCTTCAGCTGGGTGGCACAGTGATTGGAAGTGCCCGATGCAAGGACTTCCGGG
  AGCGAGAAGGACGACTCCGGGCCGCCCACAACCTGGTGAAGCGGGGGATCACCAATCTGTGTGTCATCGG
  AGGCGATGGCAGCCTCACTGGGGCTGACACTTTCCGTTCAGAGTGGAGCGACTTGCTGAATGATTTCCAG
  AAAGACGGGAAGATCACAGCCGAGGAGGCTACAAAGTCCAGCTACCTGAACATCGTGGGCCTGGTTGGCT
  CAATCGACAATGACTTCTGTGGCACTGATATGACCATTGGTACTGACTCTGCCCTGCACAGGATTGTGGA
  GATCGTAGACGCCATCACCACCACGGCTCAGAGCCACCAGAGGACGTTTGTGTTAGAAGTGATGGGCCGC
  CACTGTGGATACCTGGCCCTTGTCACCTCTCTGTCCTGTGGGGCCGACTGGGTTTTTATTCCTGAGTGTC
  CGCCAGACGATGACTGGGAAGAACACCTTTGTCGCCGGCTCAGTGAGACAAGGACCCGTGGCTCTCGTCT
  CAACATCATCATTGTTGCTGAAGGTGCCATCGACAAGAACGGGAAGCCAATCACCTCAGAAGACATCAAG
  AACCTGGTGGTGAAGCGCCTTGGATATGACACCCGGGTCACTGTTCTGGGACATGTACAGCGGGGTGGGA
  CACCATCAGCCTTTGACCGGATCCTGGGCAGCAGGATGGGTGTGGAAGCAGTGATGGCACTTTTGGAGGG
  GACCCCAGACACCCCAGCCTGTGTGGTGAGCCTCTCTGGTAACCAGGCTGTGCGTTTGCCCCTCATGGAG
  TGCGTGCAGGTGACCAAAGACGTGACCAAGGCTATGGATGAGAAGAGATTTGATGAAGCCATTAAGCTGA
  GAGGCCGGAGCTTCATGAACAACTGGGAGGTATACAAGCTTCTAGCTCATGTCAGACCCCCAGTCTCTAA
  GGGTGGGTTGCACACAGTGGCCGTGATGAATGTGGGGGCCCCAGCTGCTGGCATGAATGCCGCTGTTCGC
  TCTACCGTGAGGATTGGCCTTATCCAGGGCAACCGGGTGCTGGTCGTACATGATGGCTTTGAGGGTCTGG
  CCAAAGGTCAGATTGAGGAGGCTGGCTGGAGCTATGTTGGAGGCTGGACTGGTCAAGGTGGTTCCAAACT
  TGGTACTAAAAGGACTCTCCCCAAGAAGAACCTGGAACAGATCAGTGCCAACATAACCAAGTTTAACATC
  CAGGGCCTTGTCATCATTGGGGGCTTTGAGGCTTACACAGGGGGCTTGGAGCTGATGGAAGGCAGGAAGC
  AGTTTGATGAGCTCTGCATCCCGTTTGTGGTCATTCCGGCCACGGTTTCCAATAACGTCCCTGGGTCAGA
  CTTCAGCATCGGGGCTGACACAGCACTGAACACCATCTGCACGACTTGTGACCGAATCAAGCAGTCTGCA
  GCAGGCACCAAACGTCGGGTGTTTATCATCGAGACTATGGGTGGCTACTGTGGCTACCTGGCCACCATGG
  CAGGGCTGGCCGCTGGGGCTGATGCTGCCTACATTTTTGAGGAACCCTTCACCATTCGCGATCTCCAGGT
  GAATGTTGAACATCTGGTGCAGAAGATGAAAACAACTGTGAAGAGAGGCCTGGTGCTGAGGAATGAGAAA
  TGCAATGAGAACTACACTACTGACTTCATTTTCAACCTGTACTCTGAGGAGGGGAAGGGCATCTTTGACA
  GCAGGAAGAACGTGCTTGGCCACATGCAGCAGGGTGGAAGCCCAACTCCCTTTGACAGGAATTTTGCCAC
  TAAGATGGGTGCTAAGGCTATGAACTGGATGTCTGGGAAAATCAAGGAGAGTTACCGTAATGGACGGATC
  TTTGCCAACACCCCTGACTCAGGCTGCGTTCTGGGGATGCGTAAGAGGGCCCTGGTCTTTCAGCCAGTAA
  CTGAGCTGAAGGACCAGACAGACTTTGAACACCGAATCCCCAAAGAACAGTGGTGGCTGAAGCTGAGGCC
  AATCCTCAAAATCCTAGCCAAGTACGAGATTGATCTGGACACCTCCGACCACGCCCACCTGGAGCACATT
  TCAAGGAAACGGTCTGGAGAAGCCGCCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221898 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATW
  ESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDFQ
  KDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGR
  HCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIK
  NLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLME
  CVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVR
  STVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNI
  QGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSA
  AGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEK
  CNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRI
  FANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHI
  SRKRSGEAAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163488
• ORF Size: 2343 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163488.1, NP_001156960.1
• RefSeq Size: 2830 bp
• RefSeq ORF: 2343 bp
• Locus ID: 18642
• UniProt ID: P47857
• Cytogenetics: 15 F1
• MW: 85.3 kDa
• Gene Summary: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[UniProtKB/Swiss-Prot Function]