Prpf40a (NM_018785) Mouse Tagged ORF Clone

CAT#: MR221839

Prpf40a (Myc-DDK-tagged) - Mouse PRP40 pre-mRNA processing factor 40 homolog A (yeast) (Prpf40a)

Product Images

Circular map for MR221839

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prpf40a
• Synonyms: 2810012K09Rik; FBP-11; FBP11; Fnbp3; Fnbp11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221839 representing NM_018785
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCCGGGGACTGGAGCCGAGCGTGGAGGCCTCATGGTGAGTGAAATGGAGAGCCAACCTCCCTCGC
  GGGGTCCCGGGGACGGGGAGCGGAGATTGTCCGGCTCAAACCTGTGCTCCAGTTCTTGGGTCTCTGCTGA
  CGGCTTCCTGAGGAGACGGCCCTCGATGGGGCATCCTGGCATGCATTATGCACCAATGGGCATGCACCCT
  ATGGGTCAGAGAGCAAACATGCCTCCTGTACCTCATGGAATGATGCCACAGATGATGCCCCCTATGGGAG
  GGCCTCCAATGGGACAAATGCCTGGAATGATGTCTTCAGTAATGTCAGGAATGATGATGTCTCATATGTC
  TCAGGCTTCCATGCAACCTGCCTTACCGCCTGGAGTAAATAGTATGGATGTAGCAGCAGGTGCAGCATCT
  GGTGCAAAATCAATGTGGACAGAACATAAATCACCTGATGGAAGGACTTATTATTACAATACTGAAACAA
  AACAGTCTACCTGGGAAAAGCCAGATGATCTTAAAACACCTGCTGAGCAACTATTGTCTAAATGCCCATG
  GAAAGAGTACAAATCTGATTCCGGGAAGCCTTATTACTATAATTCTCAAACAAAGGAGTCCCGCTGGGCC
  AAACCTAAGGAACTTGAAGATCTTGAAGGATACCAGAATACCATTGTTGCTGGAGGTCTTATTACAAAAT
  CAAACCTGCATGCCATGATCAAAGCTGAAGAAAGCAGCAAGCAAGAAGAATGTACTACAGCATCAACAGC
  CCCAGTTCCTACAACAGAAATTCCTACCACTATGAGCACCATGGCTGCTGCAGAAGCAGCGGCTGCTGTT
  GTTGCAGCTGCTGCAGCTGCTGCAGCAGCAGCTAATGCCAATACTTCCACCACACCTACTAATACTGTGG
  GAAGTGTTCCAGTTGCTCCTGAACCTGAGGTTACTTCCATTGTTGCTACTGCTGTAGATAATGAAAATAC
  AGTAACTGTATCAACTGAAGAGCAAGCACAGCTTGCTAATACCACAGCTATTCAAGATCTCAGTGGTGAT
  ATATCCAGTAACACTGGAGAGGAACCAGCTAAACAAGAAACTGTCTCCGATTTTACTCCTAAAAAAGAAG
  AGGAAGAGAGCCAACCAGCAAAAAAAACATATACTTGGAATACAAAGGAGGAGGCAAAGCAAGCATTTAA
  AGAATTATTGAAAGAAAAGCGGGTGCCCTCCAATGCTTCATGGGAGCAGGCTATGAAAATGATCATAAAT
  GATCCTCGGTACAGTGCTTTAGCAAAGCTGAGTGAGAAAAAGCAGGCCTTTAATGCCTATAAAGTCCAGA
  CGGAGAAAGAGGAAAAAGAAGAAGCAAGATCAAAATACAAAGAAGCTAAGGAATCTTTTCAGCGTTTTCT
  TGAAAATCATGAGAAAATGACTTCCACAACCAGATACAAAAAAGCTGAGCAAATGTTTGGGGAGATGGAA
  GTTTGGAATGCAATTTCAGAGCGTGATCGTCTAGAAATCTATGAAGATGTTTTGTTCTTCCTTTCAAAGA
  AAGAAAAGGAACAAGCAAAGCAGCTACGAAAGAGAAATTGGGAAGCCTTAAAAAACATACTTGACAATAT
  GGCTAACGTTACATACTCTACCACTTGGTCTGAAGCCCAGCAGTATCTGATGGATAATCCAACGTTCGCA
  GAGGATGAAGAGTTACAGAACATGGATAAAGAAGATGCATTAATATGCTTTGAGGAACACATCCGGGCTT
  TAGAAAAGGAAGAAGAAGAAGAAAAACAGAAGACTTTGTTGAGAGAAAGGAGGAGGCAGCGTAAAAATAG
  GGAATCTTTTCAGATCTTTCTGGACGAGTTGCACGAGCATGGGCAGCTGCACTCTATGTCCTCTTGGATG
  GAGCTGTATCCCACCATCAGCTCTGACATTCGCTTCACTAACATGCTGGGTCAGCCGGGATCAACTGCAC
  TTGATCTTTTCAAGTTTTATGTTGAAGATCTTAAAGCACGATATCATGATGAAAAGAAGATAATAAAAGA
  CATTCTGAAGGATAAAGGATTTGTAGTTGAAGTAAACACTACTTTTGAAGATTTTGTGGCAATAATCAGT
  TCAACCAAAAGATCAACTACTTTGGATGCTGGAAATATCAAGTTGGCTTTCAATAGTTTACTAGAAAAGG
  CAGAAGCCCGTGAACGTGAGAGAGAAAAAGAAGAGGCTCGGAAAATGAAACGGAAAGAGTCTGCCTTTAA
  GAGTATGTTGAAACAGGCTACCCCTCCAATAGAACTGGATGCTGTCTGGGAAGATATCCGGGAAAGATTT
  GTAAAGGAACCAGCGTTTGAGGATATAACTCTAGAATCTGAAAGAAAAAGAATATTTAAAGATTTCATGC
  ATGTGCTTGAGCATGAATGTCAGCATCATCATTCAAAGAACAAGAAACATTCTAAGAAATCTAAAAAGCA
  CCATAGGAAACGCTCCCGCTCTCGATCGGGATCAGAGTCAGATGATGACGACAGCCATTCAAAGAAAAAA
  AGGCAGCGATCAGAGTCTCATTCTGCTTCAGAGCGTTCGTCCAGTGCTGAGTCTGAGAGAAGTTACAAGA
  AGTCAAAAAAGCATAAGAAGAAAAGCAAAAAGAGGAGGCATAAATCTGACTCTCCAGAATCAGATACTGA
  ACGAGAAAAGGATAAAAAAGAAAAAGATCGGGACAGTGAAAAAGACAGAAGTAGACAGAGATCAGAATCA
  AAACACAAATCACCTAAGAAAAAGACTGGAAAGGATTCTGGTAACTGGGATACTTCTGGCAGTGAACTGA
  GTGAAGGGGAATTGGAAAAGCGCAGAAGAACCCTTTTGGAGCAACTGGATGATGATCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221839 representing NM_018785
  Red=Cloning site Green=Tags(s)
  
  MRPGTGAERGGLMVSEMESQPPSRGPGDGERRLSGSNLCSSSWVSADGFLRRRPSMGHPGMHYAPMGMHP
  MGQRANMPPVPHGMMPQMMPPMGGPPMGQMPGMMSSVMSGMMMSHMSQASMQPALPPGVNSMDVAAGAAS
  GAKSMWTEHKSPDGRTYYYNTETKQSTWEKPDDLKTPAEQLLSKCPWKEYKSDSGKPYYYNSQTKESRWA
  KPKELEDLEGYQNTIVAGGLITKSNLHAMIKAEESSKQEECTTASTAPVPTTEIPTTMSTMAAAEAAAAV
  VAAAAAAAAAANANTSTTPTNTVGSVPVAPEPEVTSIVATAVDNENTVTVSTEEQAQLANTTAIQDLSGD
  ISSNTGEEPAKQETVSDFTPKKEEEESQPAKKTYTWNTKEEAKQAFKELLKEKRVPSNASWEQAMKMIIN
  DPRYSALAKLSEKKQAFNAYKVQTEKEEKEEARSKYKEAKESFQRFLENHEKMTSTTRYKKAEQMFGEME
  VWNAISERDRLEIYEDVLFFLSKKEKEQAKQLRKRNWEALKNILDNMANVTYSTTWSEAQQYLMDNPTFA
  EDEELQNMDKEDALICFEEHIRALEKEEEEEKQKTLLRERRRQRKNRESFQIFLDELHEHGQLHSMSSWM
  ELYPTISSDIRFTNMLGQPGSTALDLFKFYVEDLKARYHDEKKIIKDILKDKGFVVEVNTTFEDFVAIIS
  STKRSTTLDAGNIKLAFNSLLEKAEAREREREKEEARKMKRKESAFKSMLKQATPPIELDAVWEDIRERF
  VKEPAFEDITLESERKRIFKDFMHVLEHECQHHHSKNKKHSKKSKKHHRKRSRSRSGSESDDDDSHSKKK
  RQRSESHSASERSSSAESERSYKKSKKHKKKSKKRRHKSDSPESDTEREKDKKEKDRDSEKDRSRQRSES
  KHKSPKKKTGKDSGNWDTSGSELSEGELEKRRRTLLEQLDDDQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018785
• ORF Size: 2859 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018785.2, NP_061255.1
• RefSeq Size: 3565 bp
• RefSeq ORF: 2862 bp
• Locus ID: 56194
• UniProt ID: Q9R1C7
• Cytogenetics: 2 C1.1
• MW: 108.5 kDa
• Gene Summary: Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]