Rims1 (NM_053270) Mouse Tagged ORF Clone

CAT#: MR221642

Rims1 (Myc-DDK-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 1

Product Images

Circular map for MR221642

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rims1
• Synonyms: C030033M19Rik; mKIAA0340; Rab3ip1; Rim; RIM1; RIM1a; RIM1alpha; Serg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221642 representing NM_053270
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCGGCCGTGGGGCCCCGAGGTCCTCGCCCACCCACGGTGCCTCCCCCTATGCAAGAACTGCCCG
  ACCTGAGCCACCTGACCGAGGAGGAGAGGAACATTATCATGGCAGTGATGGACCGGCAGAAGGAAGAGGA
  GGAAAAAGAAGAGGCCATGCTCAAGTGTGTTGTCAGGGACATGGCGAAGCCTGCTGCCTGCAAAACACCA
  AGAAATGCTGAAAGCCAGCCCCATCAACCACCACTGAACATTTTCAGATGTGTCTGTGTTCCCAGAAAGC
  CAAGCAGCGAAGAGGGAGGCCCAGACAGAAACTGGAGATTGCATCAACAGTTTGAAAGCTACAAGGAACA
  AGTGAGAAAAATCGGAGAGGAAGCCAGGCGTTACCAGGGCGAGCACAAAGATGATGCCCCGACGTGTGGA
  ATCTGTCATAAGACAAAGTTTGCTGATGGATGTGGCCATCTCTGCTCCTATTGTCGCACCAAGTTCTGTG
  CACGCTGTGGAGGCCGAGTGTCTCTGCGATCGAACAATGAGGACAAAGTGGTTATGTGGGTATGCAATTT
  ATGTCGAAAGCAACAAGAGATATTAACGAAATCTGGAGCGTGGTTCTTCGGAAGTGGTCCTCAGCAGCCC
  AGTCAAGATGGGACTCTGAGTGACACGGCTACAGGTGCTGGATCTGAGGTGCCAAGAGAAAAGAAAGCTC
  GGCTCCAAGAGCGATCAAGGTCTCAGACGCCCTTGAGTACAGCAGCTGTCTCTTCCCAAGACACTGCTTC
  CCATGGTGCACCACTGGACAGGAACAAAGGGGCGGAGCCCTCACAGCAAGCCTTGGGTCCTGAACAGAAG
  CAGGCATCAAGGTCAAGAAGTGAGCCACCAAGGGAAAGGAAGAAGGCTCCAGGGCTTTCAGAGCAGAATG
  GCAAGGGAGGCCAGAAGAGTGAGCGCAAACGTGTCCCCAAGTCTGTGGTGCAACCCGGGGAAGGGACCGC
  GGATGAACGGGAGAGGAAGGAGAGGCGGGAAACCCGCAGGCTGGAGAAAGGGCGCTCTCAGGACTACCCG
  GACCGGCTCGAGAAACGCGAGGATGGCAGGGTGGCTGAAGACGAAAAGCAGAGGAAGGAGGAGGAGGGCG
  TGTCCACGCCGGAGTACACGAGCTGCGAGGACGTGGAGCTGGAGAGCGAGAGCGTGAGCGAGAAAGGTGA
  CTTGGACTACTGGTTGGATCCTGCCACGTGGCATAGCAGGGAAACGTCGCCTATTAGTTCGCATCCTGTA
  ACGTGGCAGCCATCTAAAGAGGGGGACCGATTAATCGGCCGTGTTATTCTTAACAAAAGAACAACCATGC
  CCAAAGAATCAGGTGCATTATTGGGTCTCAAGGTGGTTGGAGGAAAAATGACGGACTTAGGGCGTCTTGG
  TGCTTTCATCACCAAAGTAAAGAAGGGTAGCCTGGCAGATGTTGTCGGACACCTAAGAGCAGGGGATGAA
  GTTCTAGAGTGGAATGGTAAACCCCTGCCGGGAGCAACAAACGAAGAAGTTTACAACATTATTTTAGAAT
  CAAAATCAGAACCTCAAGTTGAAATTATTGTTTCAAGGCCTATTGGTGACATCCCCAGGATCCCTGAGAG
  TTCCCACCCTCCTCTGGAGTCCAGTTCAAGTTCCTTTGAATCTCAGAAGATGGAAAGGCCTTCCATTTCT
  GTTATTTCTCCAACCAGCCCTGGAGCTCTGAAAGATGCCCCACAAGTCTTACCAGGGCAACTCTCAGTGA
  AGCTGTGGTATGATAAGGTGGGGCACCAGCTGATTGTAAATGTTCTACAAGCAACAGATCTACCCCCTAG
  AGTAGATGGGCGTCCCAGAAATCCCTATGTAAAAATGTATTTTCTTCCAGATAGAAGCGACAAGAGTAAA
  AGAAGAACCAAAACGGTAAAGAAACTTCTAGAGCCAAAATGGAATCAAACATTTGTCTACTCACACGTAC
  ATCGTCGAGATTTTCGAGAGCGAATGTTAGAAATTACTGTGTGGGACCAGCCAAGAGTACAGGACGAGGA
  GAGTGAATTTCTAGGAGAGATCCTCATAGAATTGGAAACAGCACTTTTAGATGATGAGCCCCATTGGTAT
  AAACTGCAGACACATGATGAATCTTCACTACCTCTGCCTCAGCCATCACCGTTCATGCCCAGGCGGCATA
  TCCACGGAGAAAGCTCCAGCAAAAAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAGATTA
  TGAGGTTGATGATGGTATTGGCGTAGTGCCTCCAGTGGGTTATAGAGCTAGTGCTAGAGAGAGTAAAGCC
  ACCACGTTAACAGTGCCAGAGCAGCAAAGAACCACACATCACCGCTCACGTTCCGTGTCTCCTCATCGCG
  GCGATGATCAGGGAAGGCCTCGTTCACGTTTACCAAATGTGCCATTACAGAGGAGCTTAGATGAAATCCA
  TCCAACACGAAGGTCACGTTCTCCAACCCGACACCATGATGCCTCCCGAAGCCTGGCCGATCACAGATCA
  CGACATGCGGAAAGTCAATATTCGTCAGAGCCAGACAGTGAGCTTCTCATGCTGCCCAGAGCAAAACGAG
  GACGAAGTGCAGAATGCCTACACATGACCAGTGAACTGCAGCCCTCTCTTGACAGGGCTAGGAGTGCTAG
  TACCAACTGCTTGAGACCAGATACTAGTTTGCATTCACCAGAACGAGAAAGGGGTAGATGGTCCCCCTCC
  CTAGCTAGGAGGCGACCTGCTAGCCCCAGGATTCAAATCCAGCATGCGTCTCCGGAGAATGACAGGCACT
  CCAGAAAGTCTGAAAGATCTAGCATCCAAAAACAGTCTAGGAAAGGCACAGCCTCTGATGCAGACAGGGT
  TCTCCCACCATGCCTTTCTAGAAGGGGATACGCAATCCCAAGAGCAACCGATCAACCGGTCATTAGGGGA
  AAGCATACCACTCGCTCCCGGTCGAGCGAGCACTCTAGTATCAGAACCCTGTGTTCTATGCACCACCTTG
  CCCCCGGAGGGTCAGCGCCGCCTTCTCCGCTTCTGACAAGAACGCACCGACAAGGAAGCCCCACCCAGTC
  ACCTCCAGCTGACACATCCTTTGGCAGTCGCCGCGGAAGACAGCTCCCACAGGTGCCGGTCCGAAGCGGC
  AGTATAGAACAAGCGAGCTTAGTAGTGGAGGAACGAACGAGACAGATGAAAATGAAGGTACACCGATTTA
  AGCAGACAACAGGGTCTGGGTCTAGTCAAGAACTTGACCACGAGCAATACTCCAAGTACAACATACATAA
  AGATCAGTACAGAAGCTGTGATAACGCGTCCGCCAAGTCATCAGATAGTGATGTCAGTGATGTGTCCGCC
  ATTTCCAGAGCCAGCAGCACCTCACGCCTCAGCAGCACAAGCTTTATGTCAGAGCAGTCTGAGCGCCCCA
  GGGGCAGGATCAGTTCATTTACCCCCAAAATGCAAGGCAGACGGATGGGGACTTCAGGAAGAGCCATCAT
  CAAGAGCACCAGTGTAAGTGGAGAGATATATACACTGGAGCACAATGACGGCAGCCAGTCCGACACGGCC
  GTGGGTACCGTTGGAGCTGGTGGAAAGAAGCGGAGGTCCAGCCTGAGTGCCAAAGTGGTAGCCATTGTGT
  CTCGAAGAAGCAGAAGCACATCACAGCTCAGCCAGACAGAGTCCGGCCACAAGAAGTTAAAAAGCACCAT
  TCAGAGGAGTACGGAAACGGGAATGGCGGCTGAAATGCGGAAGATGGTGAGACAGCCCAGCCGGGAGTCC
  ACGGATGGCAGCATCAACAGTTATAGCTCAGAAGGAAACTTAATATTTCCTGGAGTTCGCGTGGGACCTG
  ACAGTCAGTTCAGTGATTTCCTTGATGGACTGGGGCCAGCCCAGCTTGTCGGCCGCCAAACCCTAGCCAC
  CCCAGCCATGGGCGATATCCAAATCGGGATGGAGGATAAGAAGGGTCAGTTGGAGGTTGAGGTTATCAGA
  GCCCGGAGCCTTACACAAAAACCTGGCTCCAAATCTACACCTGCTCCCTATGTGAAAGTTTATCTTTTGG
  AAAATGGAGCCTGTATTGCCAAAAAGAAGACAAGAATTGCACGGAAAACTCTCGATCCCTTGTATCAGCA
  GTCCCTGGTTTTTGATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGGTGACTATGGAAGA
  ATGGACCACAAATGCTTTATGGGTGTGGCTCAAATCTTGTTGGAAGAACTTGATCTGTCCAGCATGGTGA
  TTGGATGGTATAAATTGTTCCCTCCATCCTCCCTGGTGGATCCCACACTCACTCCCCTGACCCGCCGGGC
  TTCCCAATCATCACTGGAAAGTTCGTCTGGGCCTCCCTGCATCCGGTCA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221642 representing NM_053270
  Red=Cloning site Green=Tags(s)
  
  MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTP
  RNAESQPHQPPLNIFRCVCVPRKPSSEEGGPDRNWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCG
  ICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQP
  SQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTASHGAPLDRNKGAEPSQQALGPEQK
  QASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGTADERERKERRETRRLEKGRSQDYP
  DRLEKREDGRVAEDEKQRKEEEGVSTPEYTSCEDVELESESVSEKGDLDYWLDPATWHSRETSPISSHPV
  TWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDE
  VLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSIS
  VISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSK
  RRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWY
  KLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKA
  TTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSLADHRS
  RHAESQYSSEPDSELLMLPRAKRGRSAECLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPS
  LARRRPASPRIQIQHASPENDRHSRKSERSSIQKQSRKGTASDADRVLPPCLSRRGYAIPRATDQPVIRG
  KHTTRSRSSEHSSIRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSG
  SIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSA
  ISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLEHNDGSQSDTA
  VGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRES
  TDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIR
  ARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGR
  MDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLTPLTRRASQSSLESSSGPPCIRS
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053270
• ORF Size: 4389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053270.2
• RefSeq Size: 4392 bp
• RefSeq ORF: 4392 bp
• Locus ID: 116837
• UniProt ID: Q99NE5
• Cytogenetics: 1 A5
• MW: 163.2 kDa
• Gene Summary: Rab effector involved in exocytosis (PubMed:11797009). May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity (PubMed:11797009). Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function]