Prr19 (NM_001081294) Mouse Tagged ORF Clone

CAT#: MR221405

Prr19 (Myc-DDK-tagged) - Mouse proline rich 19 (Prr19)

Product Images

Circular map for MR221405

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prr19
• Synonyms: EG623131
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221405 representing NM_001081294
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCTCGGGGACCAGTCCCTCAGCCTTTCCAGCAGCTTCAGAAACCTGGCCGAATTCGCCGTCGCA
  AGACCAGGAGGGAGCGAAATAAGGCCCTGGTGAGCAGCCACAGGCCATTGGCCCGTCAGGATCCTCCCAT
  ATCCAGTCGGGATCCATGTGTGATCCTCCAGGATCCTGTGGCCTCTGCAGCCCCCAAGCTTGTTGTGATA
  ACTCAAGGCAGGTTGAGCCGGGAGCACCGGGGTCTCTTCAACCATGAGGTGAAATCCCTGGATGTGGCCC
  GGCTGCTTAACAGAGGTGCCTTGGAATCACATACCCCTCAGCTACCAACCAAGCCCTCCTGCAGTCCAGT
  TGGAGTACAAGAACCAGACCTTCAGTCAAAAGGCAAGGAGAACAAGGTACCAGGAGGCTCAGGCCCAGGC
  CCACCAAATTCTCCAGATCTTCCTGTGTTGGGGCAATTGCTGGAGGAGTTGCAGTACCAACTCATTGTGC
  CACAAGCCTTTCCCAGGAGAAACCTAGTGCAGGAGTCCAGAGACACCATCATAAGAACCTTACAGGGCTG
  CCATGGCTGTGTGCCTGATCTTGCCCTGGTGCTCCGAGGTTGCCAGCTACCCTTGCCTGAGGCCAAACCG
  AGGGTCCCTGAGAGACAGAGGATGGCATCTTCTTGTATGGAAGTTCCTGAGCATGCTCCACGGGAGGGGA
  AGCAAAGGACTCAGCAGGCAACAAAGGGGTGTGACTTTGCCATACCTCATACTTGCAACAGCACTACCCC
  TGCACACAGGGGCAGCCAGGTGCAACCTCCAGGTCACCAACTGCCATTCCTGTCCTCAGCATCATCACCA
  TCTGGAGCAGCATGGGGTCCCCCAACAGCTTTTGATATGCTGAAAAGCATCTGGCTCATAGCCACACCAC
  CCCCGCCCCCGCCCCAGCCCTGGGATGTCCGCCCACCTCAGCCCCTACCACAGCCACCATCACCCTTGTT
  ACCCAGAACATCTGCCTTGGACTGGAGCCCCAACCCTCCTGCCCCACTGCCCAGTCTCTCCTGGGTAGTA
  ACTCAGAGCAGTCCAGAGGCTTGGTCCTTCCCCCCAATGAGACTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221405 representing NM_001081294
  Red=Cloning site Green=Tags(s)
  
  MDPRGPVPQPFQQLQKPGRIRRRKTRRERNKALVSSHRPLARQDPPISSRDPCVILQDPVASAAPKLVVI
  TQGRLSREHRGLFNHEVKSLDVARLLNRGALESHTPQLPTKPSCSPVGVQEPDLQSKGKENKVPGGSGPG
  PPNSPDLPVLGQLLEELQYQLIVPQAFPRRNLVQESRDTIIRTLQGCHGCVPDLALVLRGCQLPLPEAKP
  RVPERQRMASSCMEVPEHAPREGKQRTQQATKGCDFAIPHTCNSTTPAHRGSQVQPPGHQLPFLSSASSP
  SGAAWGPPTAFDMLKSIWLIATPPPPPPQPWDVRPPQPLPQPPSPLLPRTSALDWSPNPPAPLPSLSWVV
  TQSSPEAWSFPPMRLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081294
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081294.1, NP_001074763.1
• RefSeq Size: 1293 bp
• RefSeq ORF: 1101 bp
• Locus ID: 623131
• Cytogenetics: 7 A3
• MW: 40.1 kDa