Rnase11 (NM_001011877) Mouse Tagged ORF Clone

CAT#: MR221354

Rnase11 (Myc-DDK-tagged) - Mouse ribonuclease, RNase A family, 11 (non-active) (Rnase11)

Product Images

Circular map for MR221354

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnase11
• Synonyms: Raj1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221354 representing NM_001011877
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTCTTTCTGCTGTTGCTCGCCCTTGGACTGCTTCTTGCAAAGCCTTCAGAAAGCAGAATGAAGG
  GGACCACAGAACAATTTTCACAGGAAGAGATGCAACCTGCTGCAAAACAGACTCTAGAGGAATCAGCAAA
  CTCAACCCTGTCAGACAAAAATACTGGCCTCAGCATATCCAAACATGTGATGTCTGCCACACCCCTAACA
  CCCAGAAGGCTATCTTTTATTATCCCCAAGGGAAATACTATGAGAGATGGCAGGAACTGTGTAAACAGCC
  TAAGAGTCTGGAGAACCGAGGTAGACGGGAATGCATCCTGTCAGTTGGGCAATGACTTTATACATGGCTC
  CATGGATGTGAGCCTCAGAATTCCCAAGGCCACCCGAGGGAAGTGTGAACAAACACCCAAGCCAAGCAGC
  AGTGGGAGCCTAGGACTGGAGCGTACCACGTGCAAGGTCCTTGCAGGCCACCAGTGCCTCAGGTCCCATG
  AGCACAGCATCACCTCATTAAAGAAAATCCTGACAGTGCTGGCCAGCAATTCTCTGATGAGCTGGTTAGT
  TAGTGGCTGTAAATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221354 representing NM_001011877
  Red=Cloning site Green=Tags(s)
  
  MAVFLLLLALGLLLAKPSESRMKGTTEQFSQEEMQPAAKQTLEESANSTLSDKNTGLSISKHVMSATPLT
  PRRLSFIIPKGNTMRDGRNCVNSLRVWRTEVDGNASCQLGNDFIHGSMDVSLRIPKATRGKCEQTPKPSS
  SGSLGLERTTCKVLAGHQCLRSHEHSITSLKKILTVLASNSLMSWLVSGCKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011877
• ORF Size: 576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011877.2, NP_001011877.1
• RefSeq Size: 579 bp
• RefSeq ORF: 579 bp
• Locus ID: 497113
• UniProt ID: Q5GAM9
• Cytogenetics: 14 C1
• MW: 21.3 kDa