Cwc25 (NM_026186) Mouse Tagged ORF Clone

CAT#: MR221154

Cwc25 (Myc-DDK-tagged) - Mouse CWC25 spliceosome-associated protein homolog (S. cerevisiae) (Cwc25)

Product Images

Circular map for MR221154

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cwc25
• Synonyms: 1300013D05Rik; AA473636; Ccdc49; R75228
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221154 representing NM_026186
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGCGGAGACCTGAACCTGAAGAAGAGCTGGCACCCGCAGACCCTTCGCAATGTGGAGAAAGTGT
  GGAAGGCGGAGCAGAAGCATGAAGCGGAGAGGAAGAAGATTGAGGAGTTGCAGCGAGAGCTCCGAGAGGA
  GAGAGCGCGGGAGGAGATGCAGCGCTACGCTGAGGATGTTGGGGCTGTCAAGAAAAAAGAAGAAAAGTTG
  GATTGGATGTACCAAGGTCCGGGTGGCATGGTGAACCGTGATGAGTACTTGCTGGGGCGCCCCATTGACA
  AGTATGTTTTTGAGAAGATGGAGGAGAGGGAAGCTGGCTGCTCTTCTGAGACAGGACTCCTCCCAGGCTC
  AATCTTTGCCCCGTCGGGCGCCAATTCCCTTCTTGACATGGCCAGCAAAATCCGGGAAGACCCTCTCTTC
  ATCATAAGGAAGAAAGAGGAGGAGAAAAAAAGAGAAGTGCTGAACAATCCTGTGAAAATGAAGAAAATCA
  AAGAGCTGTTGCAAATGAGCCTTGAGAAAAAGGAGAAGAAGAAGAAGAAGGAGAAGAAGAAGAAGCACAG
  GAAGCACAAACACAGAAGCTCCAGCAGCGGTGGCTCCAGCAGCGAGGATGAGCAGAGCCAGGCCCGGTCT
  CAAAAGAAGATGGCAAATTCCTTTCCTGTTTTGTCCAAAGTCCCTGGATATGGCTTACAGGTGAGGGACT
  CTGACCGGAACCGGGGGCTGCAGGGTTCTCTGGGTGAACAGAGGGCGATAAAGAACAATTCCCGGTCAAG
  AAGCTCCTCACCCCCCAGGCATGCCAGCAAGAAGAGCACCAAGGAAGAGAGGCCCCGGGACAGGAGGTCT
  CGATCCCCCAGCAGAAGGTCCCGGTCCCCAAGGCCCAGCAAACCGCATACCTCTAAGGTAAACAGGAAAG
  AGAGAGACAGCCCATCACCTAAGAAGGAGGCCTACCAGAGGCGGCATGCTTCTGGGTACACCAGAAAACT
  CTCAGCAGAGGAACTAGAGCGGAAACGGCAGGAGATGATGGAAAATGCCAAGTGGAGGGAGGAGGAGAGG
  CTCAACACCCTCAAGAGGCACGCCAGGGAGGACGAGCGGGAGCACAGGCTGGAGAGGTTGGACTCTCGGT
  CCGGCAAGTTCCTCCACCGCATGAAGCTGGAGAGTGCGTCGACCTCCTCCCTGGAGGACCGCGTGAAGCG
  CAACATCCATTCCCTACAGCGGACGTCGGTGGCCCTGGAGAAGAACTTCATGAGGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221154 representing NM_026186
  Red=Cloning site Green=Tags(s)
  
  MGGGDLNLKKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEEKL
  DWMYQGPGGMVNRDEYLLGRPIDKYVFEKMEEREAGCSSETGLLPGSIFAPSGANSLLDMASKIREDPLF
  IIRKKEEEKKREVLNNPVKMKKIKELLQMSLEKKEKKKKKEKKKKHRKHKHRSSSSGGSSSEDEQSQARS
  QKKMANSFPVLSKVPGYGLQVRDSDRNRGLQGSLGEQRAIKNNSRSRSSSPPRHASKKSTKEERPRDRRS
  RSPSRRSRSPRPSKPHTSKVNRKERDSPSPKKEAYQRRHASGYTRKLSAEELERKRQEMMENAKWREEER
  LNTLKRHAREDEREHRLERLDSRSGKFLHRMKLESASTSSLEDRVKRNIHSLQRTSVALEKNFMRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026186
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026186.4, NP_080462.2
• RefSeq Size: 3326 bp
• RefSeq ORF: 1251 bp
• Locus ID: 67480
• UniProt ID: Q9DBF7
• Cytogenetics: 11 D
• MW: 49.3 kDa
• Gene Summary: Involved in pre-mRNA splicing as component of the spliceosome.[UniProtKB/Swiss-Prot Function]