Atp2b1 (NM_026482) Mouse Tagged ORF Clone

CAT#: MR221085

Atp2b1 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 1 (Atp2b1)

Product Images

Circular map for MR221085

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp2b1
• Synonyms: 2810442I22Rik; E130111D10Rik; Pmca1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221085 representing NM_026482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACATGGCCAACAACTCAGTTGCGTATAGCGGTGTGAAGAACTCTTTGAAAGAAGCTAACCATG
  ATGGAGACTTTGGAATTACGCTTACAGAGCTGCGAGCTCTCATGGAGCTCAGATCCACGGATGCATTACG
  GAAAATACAGGAGAGCTATGGAGATGTCTATGGCATTTGCACCAAGTTGAAAACATCTCCCAATGAAGGT
  TTAAGTGGAAATCCTGCAGATTTAGAAAGAAGAGAAGCAGTGTTTGGAAAGAACTTTATACCTCCTAAGA
  AGCCAAAAACCTTTCTTCAGTTAGTCTGGGAAGCATTACAAGATGTCACTTTAATTATACTAGAAATTGC
  AGCCATAGTATCATTGGGCCTTTCTTTTTATCAACCTCCGGAAGGGGATAATGCACTTTGTGGAGAAGTT
  TCTGTTGGGGAAGAAGAAGGTGAAGGAGAAACGGGGTGGATTGAAGGAGCTGCGATCCTCTTGTCGGTGG
  TGTGTGTGGTGTTGGTGACGGCTTTCAACGACTGGAGCAAGGAGAAGCAGTTCCGAGGCCTGCAGAGTCG
  AATTGAGCAGGAACAGAAGTTCACCGTCATCAGGGGTGGGCAGGTCATCCAGATACCTGTAGCTGACATT
  ACTGTTGGAGACATTGCCCAAGTGAAGTACGGTGACCTTCTTCCAGCTGATGGCATACTGATTCAAGGCA
  ATGACCTGAAGATTGATGAGAGCTCATTGACTGGTGAATCTGATCATGTTAAGAAGTCTCTAGATAAGGA
  TCCCTTACTTCTTTCAGGTACTCATGTGATGGAAGGTTCTGGAAGAATGGTGGTCACTGCTGTAGGTGTG
  AATTCTCAAACAGGAATCATCTTCACCTTACTTGGAGCTGGGGGTGAAGAAGAGGAAAAGAAAGATGAGA
  AGAAGAAGGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAGAATCGCAACAAAGCAAAAGCCCAGGA
  CGGCGCGGCCATGGAGATGCAACCTCTGAAGAGTGAAGAAGGAGGGGACGGTGATGAGAAAGACAAGAAG
  AAAGCAAATCTGCCAAAAAAGGAAAAATCCGTGTTACAGGGGAAACTCACAAAGCTGGCTGTTCAGATTG
  GCAAAGCAGGTCTGCTGATGTCTGCCATCACAGTTATCATCCTAGTGTTGTATTTTGTGATCGATACCTT
  CTGGGTTCAGAAAAGACCATGGCTTGCAGAGTGCACACCGATCTACATCCAGTACTTCGTGAAGTTCTTC
  ATTATTGGAGTCACAGTTCTAGTGGTCGCCGTGCCGGAAGGTCTTCCGCTGGCAGTCACCATCTCACTGG
  CTTACTCAGTCAAGAAAATGATGAAGGATAATAACTTAGTGAGACATCTGGATGCTTGTGAGACCATGGG
  GAATGCTACAGCTATCTGTTCAGATAAAACAGGAACCCTGACGATGAACAGGATGACGGTTGTTCAAGCT
  TACATAAATGAAAAGCATTATAAAAAGGTTCCGGAACCAGAAGCCATCCCACCAAATATCCTGTCCTACC
  TGGTAACGGGGATTTCTGTGAATTGTGCTTATACGTCAAAAATATTGCCACCGGAGAAAGAGGGTGGATT
  ACCCCGCCATGTGGGTAACAAAACAGAGTGTGCCTTGTTGGGGTTTCTCTTGGATTTAAAACGGGATTAT
  CAAGATGTTAGAAATGAAATACCAGAGGAAGCACTGTACAAAGTCTATACCTTCAATTCTGTTAGGAAGT
  CCATGAGCACAGTCCTGAAAAACTCAGATGGAAGTTTCCGCATCTTTAGCAAGGGTGCCTCCGAGATCAT
  TCTGAAAAAGTGTTTCAAAATCCTGAGTGCTAATGGCGAGGCAAAAGTATTCCGACCCAGAGACCGCGAT
  GACATTGTGAAAACTGTGATCGAGCCCATGGCATCAGAAGGCCTGAGGACCATATGCCTAGCGTTCAGAG
  ACTTCCCAGCGGGGGAGCCAGAACCAGAGTGGGATAATGAGAATGATGTCGTCACCGGCCTTACGTGTAT
  CGCGGTTGTGGGGATTGAAGATCCTGTGAGGCCTGAGGTACCAGAGGCAATAAAGAAATGTCAGAGGGCT
  GGAATTACTGTGCGCATGGTCACTGGTGATAATATTAACACTGCTCGGGCCATTGCTACCAAATGTGGTA
  TTTTACACCCAGGGGAAGATTTCCTATGCTTAGAAGGTAAAGATTTTAACCGAAGAATACGAAATGAAAA
  AGGAGAGATTGAACAAGAAAGGATAGACAAGATTTGGCCAAAGCTTCGAGTACTTGCAAGATCATCTCCC
  ACTGACAAACATACACTGGTGAAAGGTATAATTGACAGCACAGTCTCAGAGCAACGACAGGTTGTAGCTG
  TAACGGGTGATGGTACAAATGACGGGCCTGCACTGAAGAAAGCAGACGTTGGCTTTGCAATGGGCATTGC
  TGGAACTGATGTGGCTAAAGAAGCGTCTGATATTATTCTCACAGATGACAACTTTACAAGCATTGTCAAA
  GCAGTTATGTGGGGACGAAATGTCTATGACAGCATCTCAAAATTCCTTCAGTTCCAGCTTACTGTTAATG
  TAGTAGCAGTGATTGTTGCTTTCACGGGCGCTTGTATTACTCAAGACTCGCCACTTAAGGCGGTGCAGAT
  GCTCTGGGTAAACCTCATCATGGACACGCTGGCTTCCCTGGCTCTGGCTACGGAACCACCCACCGAGTCA
  CTCCTGCTTCGGAAGCCTTATGGTAGAAATAAGCCTCTCATCTCACGCACGATGATGAAGAACATCTTGG
  GCCATGCATTCTATCAGCTCGTAGTGGTCTTTACACTCTTATTTGCTGGAGAAAAGTTTTTTGATATCGA
  CAGCGGGAGGAATGCGCCTCTGCACGCTCCCCCGTCAGAACATTACACCATCGTGTTCAATACCTTTGTG
  CTGATGCAGCTCTTCAATGAAATAAACGCCCGGAAAATTCACGGGGAAAGAAATGTGTTTGAAGGAATCT
  TCAATAACGCCATCTTCTGCACCATTGTCCTGGGCACCTTTGTGGTGCAGATAATAATTGTGCAGTTTGG
  CGGGAAGCCTTTCAGCTGCTCAGAACTTTCAATAGAGCAATGGCTGTGGTCGATATTCCTGGGAATGGGG
  ACCTTACTCTGGGGCCAGCTTATTTCAACCATTCCAACCAGCCGCTTAAAGTTTCTAAAAGAAGCTGGTC
  ATGGAACCCAGAAGGAGGAGATACCTGAGGAGGAATTAGCAGAGGATGTTGAAGAGATTGACCATGCCGA
  AAGGGAGTTGCGGCGTGGCCAGATCTTGTGGTTTAGAGGCCTGAACAGAATCCAAACACAGATCCGAGTG
  GTGAATGCATTTCGTAGTTCTTTATACGAAGGGTTAGAGAAGCCAGAATCAAGAAGTTCAATCCACAACT
  TTATGACACACCCTGAGTTTAGGATAGAAGATTCAGAGCCGCACATCCCCCTTATTGATGACACTGATGC
  TGAAGACGACGCCCCAACAAAACGCAACTCCAGCCCTCCGCCCTCTCCTAACAAAAATAACAATGCTGTT
  GACAGCGGAATTCACCTTACAATAGAAATGAACAAGTCTGCTACCTCTTCATCCCCAGGAAGCCCACTAC
  ATAGTTTGGAAACATCACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221085 representing NM_026482
  Red=Cloning site Green=Tags(s)
  
  MGDMANNSVAYSGVKNSLKEANHDGDFGITLTELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEG
  LSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEV
  SVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADI
  TVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGV
  NSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKK
  KANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFF
  IIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQA
  YINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGFLLDLKRDY
  QDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSFRIFSKGASEIILKKCFKILSANGEAKVFRPRDRD
  DIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDVVTGLTCIAVVGIEDPVRPEVPEAIKKCQRA
  GITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSP
  TDKHTLVKGIIDSTVSEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVK
  AVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTES
  LLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFV
  LMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMG
  TLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQIRV
  VNAFRSSLYEGLEKPESRSSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNSSPPPSPNKNNNAV
  DSGIHLTIEMNKSATSSSPGSPLHSLETSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026482
• ORF Size: 3660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026482.2, NP_080758.1
• RefSeq Size: 7130 bp
• RefSeq ORF: 3663 bp
• Locus ID: 67972
• UniProt ID: G5E829
• Cytogenetics: 10 C3
• MW: 135.2 kDa
• Gene Summary: Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis (PubMed:22311909, PubMed:16956963, PubMed:28827723, PubMed:26392310, PubMed:29950683, PubMed:24805951, PubMed:23266958). Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction (PubMed:24805951, PubMed:29950683, PubMed:22311909). Positively regulates bone mineralization through absorption of calcium from the intestine (PubMed:23266958, PubMed:26392310). Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (PubMed:23266958). Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function]