Il12rb1 (NM_008353) Mouse Tagged ORF Clone

CAT#: MR220916

Il12rb1 (Myc-DDK-tagged) - Mouse interleukin 12 receptor, beta 1 (Il12rb1)

Product Images

Circular map for MR220916

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il12rb1
• Synonyms: CD212; IL-12R[b]
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220916 representing NM_008353
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATGATGGGGCTGGCAGGGACCAGCAAACACATCACCTTCCTCCTGCTTTGCCAGCTAGGCGCCT
  CAGGCCCAGGTGATGGCTGCTGCGTTGAGAAGACATCGTTCCCAGAGGGAGCCTCAGGCTCACCCTTAGG
  ACCCAGGAACTTGAGTTGCTACAGGGTTTCCAAGACAGACTATGAGTGCTCCTGGCAGTATGATGGCCCT
  GAGGACAATGTTTCTCACGTCCTGTGGTGCTGCTTTGTCCCTCCGAACCATACCCACACCGGCCAGGAGC
  GCTGCCGCTACTTCTCCTCAGGCCCAGACCGCACTGTGCAGTTCTGGGAACAGGACGGTATCCCTGTGCT
  GTCCAAGGTCAACTTCTGGGTGGAGTCTCGGCTTGGGAACCGAACCATGAAGTCCCAGAAGATATCCCAG
  TACCTGTACAACTGGACCAAGACGACCCCTCCCCTGGGACACATCAAGGTGTCACAATCACACCGGCAGT
  TGCGAATGGACTGGAATGTGTCTGAAGAGGCCGGTGCTGAGGTACAGTTCAGGCGCCGTATGCCCACAAC
  GAATTGGACCTTGGGTGACTGCGGACCTCAGGTTAACTCTGGCTCAGGTGTGCTTGGTGACATTCGTGGG
  AGCATGTCTGAGTCCTGCCTCTGCCCTTCTGAGAACATGGCCCAAGAGATCCAGATACGGAGGAGGAGGC
  GGCTCTCCTCAGGAGCCCCTGGAGGTCCCTGGAGTGATTGGAGCATGCCTGTGTGTGTTCCACCTGAAGT
  CCTTCCCCAGGCCAAGATTAAGTTCTTGGTGGAGCCCCTGAACCAAGGTGGAAGGAGGCGTCTAACCATG
  CAAGGACAGTCACCACAGCTGGCAGTCCCGGAAGGCTGCCGAGGCAGGCCTGGTGCGCAGGTGAAGAAGC
  ACTTGGTGCTGGTGCGCATGCTGTCCTGCAGGTGCCAGGCTCAGACCTCGAAGACCGTGCCCCTGGGCAA
  GAAGCTGAACCTCTCCGGGGCCACCTATGACCTGAATGTGCTCGCCAAAACTCGTTTCGGTCGCAGCACC
  ATCCAGAAGTGGCACCTTCCTGCCCAAGAGCTCACAGAGACAAGAGCCCTGAATGTCAGCGTGGGAGGCA
  ACATGACATCCATGCAGTGGGCAGCCCAGGCTCCCGGCACCACCTACTGCCTTGAGTGGCAGCCATGGTT
  CCAGCACAGGAACCACACACACTGTACCCTGATTGTACCAGAAGAAGAGGATCCTGCCAAGATGGTGACA
  CACAGCTGGAGCTCTAAACCTACCCTGGAGCAGGAGGAATGTTACCGCATCACAGTGTTCGCCTCCAAGA
  ACCCCAAGAATCCCATGCTGTGGGCCACAGTCCTGTCCAGTTACTACTTTGGGGGTAACGCCTCGAGAGC
  CGGTACTCCACGACACGTGTCGGTGAGGAACCAAACCGGGGACTCGGTGTCCGTGGAGTGGACGGCGTCA
  CAGCTGAGCACCTGCCCGGGGGTCCTGACGCAATACGTCGTGCGCTGCGAGGCTGAAGACGGCGCGTGGG
  AGTCAGAGTGGCTCGTGCCACCCACTAAAACCCAAGTGACACTTGACGGACTGCGCAGCCGAGTAATGTA
  CAAGGTTCAGGTGCGAGCCGACACTGCGAGGCTCCCGGGTGCCTGGAGTCATCCCCAGCGCTTTAGCTTT
  GAGGTGCAGATTTCCCGTTTATCCATCATTTTCGCGTCTCTGGGAAGCTTCGCCAGCGTCCTCCTCGTGG
  GCAGTCTCGGATACATTGGCTTAAACAGGGCCGCCTGGCACTTGTGCCCACCCCTGCCTACACCCTGTGG
  CAGCACTGCCGTGGAGTTCCCTGGCAGCCAGGGCAAGCAGGCTTGGCAGTGGTGCAACCCTGAGGACTTC
  CCGGAGGTGTTGTACCCGCGAGATGCGCTGGTGGTCGAGATGCCCGGAGACAGAGGCGACGGGACAGAGT
  CGCCCCAGGCCGCCCCTGAGTGCGCCCTGGACACAAGGCGGCCCTTGGAGACTCAGAGGCAGAGGCAGGT
  GCAGGCACTGTCAGAGGCCAGGCGCCTGGGCCTGGCTAGGGAGGACTGTCCCCGTGGTGACCTGGCCCAC
  GTGACACTCCCGCTGCTCCTGGGAGGTGTGACCCAGGGAGCCTCTGTACTTGACGATCTTTGGAGGACCC
  ATAAGACTGCGGAGCCGGGACCGCCCACTTTGGGGCAAGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220916 representing NM_008353
  Red=Cloning site Green=Tags(s)
  
  MDMMGLAGTSKHITFLLLCQLGASGPGDGCCVEKTSFPEGASGSPLGPRNLSCYRVSKTDYECSWQYDGP
  EDNVSHVLWCCFVPPNHTHTGQERCRYFSSGPDRTVQFWEQDGIPVLSKVNFWVESRLGNRTMKSQKISQ
  YLYNWTKTTPPLGHIKVSQSHRQLRMDWNVSEEAGAEVQFRRRMPTTNWTLGDCGPQVNSGSGVLGDIRG
  SMSESCLCPSENMAQEIQIRRRRRLSSGAPGGPWSDWSMPVCVPPEVLPQAKIKFLVEPLNQGGRRRLTM
  QGQSPQLAVPEGCRGRPGAQVKKHLVLVRMLSCRCQAQTSKTVPLGKKLNLSGATYDLNVLAKTRFGRST
  IQKWHLPAQELTETRALNVSVGGNMTSMQWAAQAPGTTYCLEWQPWFQHRNHTHCTLIVPEEEDPAKMVT
  HSWSSKPTLEQEECYRITVFASKNPKNPMLWATVLSSYYFGGNASRAGTPRHVSVRNQTGDSVSVEWTAS
  QLSTCPGVLTQYVVRCEAEDGAWESEWLVPPTKTQVTLDGLRSRVMYKVQVRADTARLPGAWSHPQRFSF
  EVQISRLSIIFASLGSFASVLLVGSLGYIGLNRAAWHLCPPLPTPCGSTAVEFPGSQGKQAWQWCNPEDF
  PEVLYPRDALVVEMPGDRGDGTESPQAAPECALDTRRPLETQRQRQVQALSEARRLGLAREDCPRGDLAH
  VTLPLLLGGVTQGASVLDDLWRTHKTAEPGPPTLGQEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008353
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008353.2, NP_032379.2
• RefSeq Size: 2873 bp
• RefSeq ORF: 2217 bp
• Locus ID: 16161
• UniProt ID: Q60837
• Cytogenetics: 8 34.21 cM
• MW: 82.2 kDa
• Gene Summary: Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.[UniProtKB/Swiss-Prot Function]