Plk2 (NM_152804) Mouse Tagged ORF Clone

CAT#: MR220913

Plk2 (Myc-DDK-tagged) - Mouse polo-like kinase 2 (Drosophila) (Plk2)

Product Images

Circular map for MR220913

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plk2
• Synonyms: Snk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220913 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCCTGCGGACTATCACCTACCAGCCGGCCGCCGGCACCAAGATGTGCGAGCAGGCTCTGGGCA
  AAGCTTGCGGCGGGGACTCAAAGAAGAAGCGACCACAGCAGCCTTCTGAAGATGGGCAGCCCCAAGCCCA
  GGTGACCCCGGCGGCCCCGCACCACCATCACCACCATTCCCACTCGGGACCCGAGATCTCGCGGATTATA
  GTCGACCCCACGACGGGGAAGCGCTACTGCCGGGGCAAAGTGCTGGGCAAGGGTGGATTTGCAAAGTGTT
  ACGAAATGACAGATCTGACAAACAACAAAGTCTACGCTGCAAAAATTATTCCTCACAGCAGAGTAGCTAA
  ACCTCATCAGAGGGAAAAGATCGACAAAGAAATCGAGCTTCACAGACTACTGCACCATAAGCATGTCGTG
  CAGTTTTACCACTACTTTGAAGACAAAGAAAACATTTACATTCTCTTGGAATACTGCAGTAGAAGGTCCA
  TGGCTCACATCTTGAAAGCAAGAAAGGTGTTGACAGAGCCAGAAGTCCGATACTACCTCAGGCAGATTGT
  GTCAGGACTCAAGTATCTTCACGAACAAGAAATCTTGCACAGGGATCTCAAGCTAGGGAACTTTATTATT
  AATGAAGCCATGGAGCTGAAGGTGGGAGACTTTGGTTTGGCAGCCAGACTGGAACCACTGGAACACAGAA
  GGAGAACAATATGTGGAACCCCAAATTATCTCTCCCCCGAAGTCCTCAACAAACAAGGACACGGCTGTGA
  ATCAGACATCTGGGCCTTAGGCTGTGTAATGTATACGATGCTGCTAGGAAGACCTCCATTCGAAACCACA
  AATCTGAAAGAAACGTACAGGTGCATAAGGGAAGCAAGGTATACCATGCCGTCCTCATTGCTGGCCCCTG
  CTAAGCACTTGATAGCTAGCATGCTGTCCAAAAACCCAGAGGACCGCCCCAGTTTGGATGACATCATTCG
  GCATGACTTCTTCCTGCAGGGTTTCACTCCGGACAGACTCTCTTCCAGCTGTTGCCACACAGTTCCAGAT
  TTCCACTTGTCAAGCCCAGCCAAGAATTTCTTTAAGAAAGCCGCAGCCGCTCTTTTTGGTGGCAAGAAGG
  ACAAAGCAAGATATAACGACACACACAATAAGGTGTCTAAGGAAGATGAAGACATTTACAAGCTTCGGCA
  TGATTTGAAGAAAGTGTCGATAACCCAGCAGCCTAGCAAACACAGAGCAGACGAGGAGCCCCAGCCGCCT
  CCCACTACTGTTGCCAGATCTGGAACGTCCGCAGTGGAAAACAAACAGCAGATTGGGGATGCAATCCGGA
  TGATAGTCAGGGGGACTCTCGGCAGCTGCAGCAGCAGCAGCGAATGCCTTGAAGACAGCACCATGGGAAG
  TGTTGCAGACACAGTGGCAAGAGTCCTTCGAGGATGTCTAGAAAACATGCCGGAAGCTGACTGTATCCCC
  AAAGAGCAGCTGAGCACGTCCTTTCAGTGGGTCACCAAGTGGGTCGACTACTCCAACAAATATGGCTTTG
  GGTACCAGCTCTCGGACCACACTGTTGGCGTCCTTTTCAACAACGGGGCTCACATGAGCCTCCTTCCGGA
  CAAAAAGACAGTTCACTATTATGCGGAACTTGGCCAATGCTCTGTTTTCCCAGCAACAGATGCCCCTGAA
  CAATTTATTAGTCAAGTGACGGTGCTGAAATACTTTTCTCATTACATGGAGGAGAACCTCATGGATGGTG
  GTGATCTCCCGAGTGTTACTGACATTCGAAGACCTCGGCTCTACCTCCTGCAGTGGTTAAAGTCTGATAA
  AGCCTTAATGATGCTCTTCAATGACGGCACATTTCAGGTGAATTTCTACCACGATCATACAAAAATCATC
  ATCTGTAACCAGAGTGAAGAATACCTTCTCACCTACATCAATGAGGACAGGATCTCTACAACTTTCAGAC
  TGACGACTCTGCTGATGTCTGGCTGTTCGTTAGAATTGAAAAATCGAATGGAATATGCCCTGAACATGCT
  CTTACAGAGATGTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220913 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELLRTITYQPAAGTKMCEQALGKACGGDSKKKRPQQPSEDGQPQAQVTPAAPHHHHHHSHSGPEISRII
  VDPTTGKRYCRGKVLGKGGFAKCYEMTDLTNNKVYAAKIIPHSRVAKPHQREKIDKEIELHRLLHHKHVV
  QFYHYFEDKENIYILLEYCSRRSMAHILKARKVLTEPEVRYYLRQIVSGLKYLHEQEILHRDLKLGNFII
  NEAMELKVGDFGLAARLEPLEHRRRTICGTPNYLSPEVLNKQGHGCESDIWALGCVMYTMLLGRPPFETT
  NLKETYRCIREARYTMPSSLLAPAKHLIASMLSKNPEDRPSLDDIIRHDFFLQGFTPDRLSSSCCHTVPD
  FHLSSPAKNFFKKAAAALFGGKKDKARYNDTHNKVSKEDEDIYKLRHDLKKVSITQQPSKHRADEEPQPP
  PTTVARSGTSAVENKQQIGDAIRMIVRGTLGSCSSSSECLEDSTMGSVADTVARVLRGCLENMPEADCIP
  KEQLSTSFQWVTKWVDYSNKYGFGYQLSDHTVGVLFNNGAHMSLLPDKKTVHYYAELGQCSVFPATDAPE
  QFISQVTVLKYFSHYMEENLMDGGDLPSVTDIRRPRLYLLQWLKSDKALMMLFNDGTFQVNFYHDHTKII
  ICNQSEEYLLTYINEDRISTTFRLTTLLMSGCSLELKNRMEYALNMLLQRCN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152804
• ORF Size: 2049 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152804.1
• RefSeq Size: 2802 bp
• RefSeq ORF: 2049 bp
• Locus ID: 20620
• UniProt ID: P53351
• Cytogenetics: 13 D2.1
• MW: 77.8 kDa
• Gene Summary: Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress.[UniProtKB/Swiss-Prot Function]