Atp1a4 (NM_013734) Mouse Tagged ORF Clone

CAT#: MR220827

Atp1a4 (Myc-DDK-tagged) - Mouse ATPase, Na+/K+ transporting, alpha 4 polypeptide (Atp1a4)

Product Images

Circular map for MR220827

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp1a4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220827 representing NM_013734
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCTGGAAAAGAAAAGGAGGTAGAGGCCCCTGGTGAGCTGAATCAGAAACCAAGACCCAGCACCA
  GGTCCTCAACTACAAATAGACAGCCGAAAATGAAAAGAAGGAAGAAAGATTTAGAAGAGCTGAAGAAGGA
  AGTGGTTATGGATGATCACAAGCTAACGTTGGATGAGCTGAGTGCCAAGTACTCTGTGGACTTGACCAAG
  GGTCTTAGCATCCTAGAAGCCCAGGATATTCTGTTTCAAAATGGACCGAATGTACTAACTCCTCCTCCAA
  CCACTCCAGAGTGGGTCAAATTCTGTAGACAACTGTTTGGTGGCTTCTCCCTCCTACTGTGGACTGGTGC
  CTGTCTCTGCTTCCTGGCCTATGGCATACATGTAAATTATTACAAAGAAAACGCCAACAAAGATAATCTG
  TACCTGGGGATTGTACTATCTGCCGTGGTCATCATTACCGGCTGCTTCTCATATTACCAGGAGGCCAAGA
  GCTCCAAGATCATGGAGTCTTTTAAGAACATGGTGCCTCAGCAAGCTCTGGTGATTCGAGATGGAGAGAA
  AATGCAAATCAATGTAAGAGATGTGGTTTTGGGAGACCTAGTAGAGGTGAAGGGAGGAGACCAAATCCCT
  GCTGATATCAGGGTTATCTCGGCGCAAGGATGCAAGGTAGACAATTCCTCCTTGACTGGGGAGTCAGAAC
  CCCAGTCTCGCTGCCCTGATTGCACTCATGAGAACCCTCTGGAGACACGGAATATCATCTTCTTCTCCAC
  TAACTGTGTAGAAGGAACAGCTCGGGGTATTGTGATTGCTACAGGAGACTACACAGTAATGGGCCGGATT
  GCTTCCCTGACATCAGGCTTGCAAATGGGCAAGACCCCGATTGCCACCGAAATCGAACACTTCATCCATT
  TGATCACAGCTGTCGCGGTCTTCCTCGGTGTTTCATTTTTTTGGCTCTCGATTATCTTGGGTTACACCTG
  GCTGGATGCTGTCATTTTCCTTATTGGTATCATTGTGGCCAATGTGCCGGAAGGGCTGCTGGCCACTGTC
  ACTGTGTGCCTGACGCTGACCGCCAAGCGCATGGCTCGCAAGAACTGCCTGGTGAAGAACCTGGAGGCCG
  TGGAGACGCTGGGCTCCACGTCCACCATCTGCTCAGACAAGACGGGCACCCTCACCCAGAACCGCATGAC
  AGTGGCGCATTTGTGGTTTGATAAGACTGTGTATGAGGCCGACACCAGTGAGGAGCAAACCACGGGGAAA
  ACGTTTCCCAAGTCCTCAGACACCTGGTTTTACCTGGCTCGAATCGCTGGCCTCTGTAACCGGGCCGACT
  TTAAACCCCATCAGGAGTCTGTTCCCATAGCTAAGCGGGCAACAACAGGGGATGCTTCCGAGTCAGCTCT
  CCTCAAATTCATTGAGCAGTCTTACAACCCCGTGAGCGAAATGAGGCAGAAAAACCCCAAGGTGGCGGAG
  ATTCCCTTTAACTCCACCAACAAGTATCAGATGTCCATCCACCTTCTGGAAGATAATTCCGAAGCCCACG
  TGCTGCTAATGAAAGGTGCCCCAGAGAGGATCTTTGATTTTTGCTCCAGTTTCCTGCTGAATGGGCAGGA
  GTACCCTATGGATGAGGAAATGAAGATGGACTTCCAGAATGCCTACATAGAACTGGGAGGTCTGGGCGAA
  CGTGTGCTGGGGTTCTGCTTCTTGAACTTGCCTAGCAATTTCTCTAAGGGGTTCCAGTTTAATACCGATG
  AGTTAAATTTTCCCATGGAAAATCTGTGTTTCGCGGGCCTCATCTCCATGATTGATCCTCCTCGGACGGC
  CGTGCCTGACGCTGTGAGCAAATGTCGAAGCGCGGGGATCAAGGTGATCATGGTAACGGGGGATCATCCC
  ATCACAGCCAAAGCTATTGCCAAAAGTGTGGGTATCATTTCAGAAGGTAACGACACGGCGGAGGACATTG
  CCGCTCGGCTGAATATCCCTATCAGTCAAGTCAATAACAAGTCTGTTAAAGCCATTGTAGTGCACGGCTC
  AGAACTGAAAGACATGGAATCCCAACAGCTGGACGACATCCTCAAGTCCTATAAGGAGATTGTCTTTGCC
  CGGACCTCTCCTCAGCAGAAACTCATCATTGTGGAGGGGTGTCAGCGACTGGGAGCCATTGTGGCAGTGA
  CTGGTGACGGGGTGAACGACTCCCCCGCACTGAAGAAGGCTGACATCGGCATTGCCATGGGCATCACAGG
  CTCTGATGTCTCTAAACAGGCGGCTGACATGATCCTTCTTGACGACAATTTTGCCTCCATTGTGACAGGC
  GTGGAGGAGGGCCGCCTCATCTTTGACAACCTGAAGAAGTCCATCGCATACACCCTGACCAGCAACATCC
  CTGAGATCACCCCCTTCCTGCTGTTCATCATCTTAAGCATACCCTTGCCTCTGGGCACCATAACCATTCT
  CTGCATCGACCTGGGCACTGACATGGTCCCTGCTATCTCCCTGGCTTATGAATCACCTGAAAGTGACATC
  ATGAAAAGGCTTCCACGGAACCCCAAAACGGACAATCTTGTGAACAACCGTCTTATTGGCATGGCCTATG
  GGCAGATTGGGATGATACAGGCTCTGGCTGGATTTTTCACCTATTTTGTGATCCTGGCGGAGAATGGTTT
  TAAGCCTCTTGATCTTCTGGGTATCCGTCTATACTGGGATGATACACAATTAAATGACCTAGAGGACAGC
  TATGGGCAGCAGTGGACCTATGAGCAGCGGAAGGTGGTGGAATTCACATGCCAAACGGCCTTCTTCATCA
  GTATCGTGATTGTGCAGTGGGCTGACCTCATCATCTGCAAGACACGCAGAAATTCACTCTTCAAGCAGGG
  CATGAAAAACAAAATCCTAATATTTGGACTCCTTGAGGAGACAGTCCTGGCAGCTTTCCTGTCCTACGTT
  CCAGGCATGGATGTGTCCCTGCGGATGTACCCACTCAAGATAAACTGGTGGTTCTGCGCACTTCCCTACA
  GTGTTCTCATCTTCGTCTATGATGAAATCAGAAAACTTATCATCCGGAGACGGCCTGGTGGATGGCTGGA
  GAAGGAGACGTACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220827 representing NM_013734
  Red=Cloning site Green=Tags(s)
  
  MEPGKEKEVEAPGELNQKPRPSTRSSTTNRQPKMKRRKKDLEELKKEVVMDDHKLTLDELSAKYSVDLTK
  GLSILEAQDILFQNGPNVLTPPPTTPEWVKFCRQLFGGFSLLLWTGACLCFLAYGIHVNYYKENANKDNL
  YLGIVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRDGEKMQINVRDVVLGDLVEVKGGDQIP
  ADIRVISAQGCKVDNSSLTGESEPQSRCPDCTHENPLETRNIIFFSTNCVEGTARGIVIATGDYTVMGRI
  ASLTSGLQMGKTPIATEIEHFIHLITAVAVFLGVSFFWLSIILGYTWLDAVIFLIGIIVANVPEGLLATV
  TVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHLWFDKTVYEADTSEEQTTGK
  TFPKSSDTWFYLARIAGLCNRADFKPHQESVPIAKRATTGDASESALLKFIEQSYNPVSEMRQKNPKVAE
  IPFNSTNKYQMSIHLLEDNSEAHVLLMKGAPERIFDFCSSFLLNGQEYPMDEEMKMDFQNAYIELGGLGE
  RVLGFCFLNLPSNFSKGFQFNTDELNFPMENLCFAGLISMIDPPRTAVPDAVSKCRSAGIKVIMVTGDHP
  ITAKAIAKSVGIISEGNDTAEDIAARLNIPISQVNNKSVKAIVVHGSELKDMESQQLDDILKSYKEIVFA
  RTSPQQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMGITGSDVSKQAADMILLDDNFASIVTG
  VEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIILSIPLPLGTITILCIDLGTDMVPAISLAYESPESDI
  MKRLPRNPKTDNLVNNRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPLDLLGIRLYWDDTQLNDLEDS
  YGQQWTYEQRKVVEFTCQTAFFISIVIVQWADLIICKTRRNSLFKQGMKNKILIFGLLEETVLAAFLSYV
  PGMDVSLRMYPLKINWWFCALPYSVLIFVYDEIRKLIIRRRPGGWLEKETYY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013734
• ORF Size: 3096 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013734.1, NP_038762.1
• RefSeq Size: 3485 bp
• RefSeq ORF: 3099 bp
• Locus ID: 27222
• UniProt ID: Q9WV27
• Cytogenetics: 1 79.54 cM
• MW: 114.9 kDa
• Gene Summary: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility (By similarity).[UniProtKB/Swiss-Prot Function]