Dcst1 (NM_029974) Mouse Tagged ORF Clone

CAT#: MR220776

Dcst1 (Myc-DDK-tagged) - Mouse DC-STAMP domain containing 1 (Dcst1)

Product Images

Circular map for MR220776

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dcst1
• Synonyms: A330106H01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220776 representing NM_029974
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCCTCTCATCAACACTTCACAGCCTAGGGATCTTTGAAAAGATTTCCAGGATAAAAGAGGTTC
  TCAAGAATAGGCTGCTGGACCTTACAAAGCGTCGGGACCAAGCACGGGAGCAACAAAGGAAACGGCCCCA
  CACCATCATACAGGGGCTGCTCCTCTGGAGTCTTCCAGTTTCCTGGATCCGGTTCCTGTGGCGACAGCCA
  GGGGAGTTTCCAGTAACTGCTTTCCTGCTAGGAGCAGGCACTGGTGGGCTCCTGGCCATAGGTCTCTTTC
  AGCTCTTGGTGAATCCCATGAACATTTATGAGGAGCAGAAGGTGGTGGCGTTATACTGCTTGGCAAGCTT
  GGGGGCCATCGGCTGGGGCACCTCCCCTCACATCCGCTGTGCCAGTCTCCTGTTGGTACCCAAGATGCTG
  GGCAAAGAAGGCAGACTCTTTGTCATGGGATATGCTTTGGCTGCTATCTACAGTGGGCCGGCGGCCAATC
  TAAGGTCCAACATCAACGAGGTGATAGCATCCCTGGGCTGCACTGTGGAGCTGCAGATCAACAACACCCG
  ATCCGCCTGGCGAGTCTCCACAGCCCCTCTGCGGGCAGTGTTCAAGGGCATGGTGGGCAGCAAAGATTCA
  TTGAATAAAGAGATTCAGAACGTCTCTACCTCCTTTGAAGAGATGGATGAACAGGTGAAGAGTGATGCTG
  GCTACTCATCTGAGGACTGGGACAAGAACAGAGAAAGCACAGAGATGTTCGGAACCTCCCGCGTCCGACC
  CTACCTGTCCACACAGATGGTGTATGAGCTGAGGACCAGGCTACGCTGCATCCATGTAGTAAATAAGGCC
  ATACTCAGCTGCTACCGCTGGTTTGATAAAAAGCATAAAAACTGCATGCGACGCATCCACCTCCCACTCT
  TCAACAACATGATCTGTGTGCCAATGAAGTTCAAGTTCCTCTGTAACATTGCCAAGGTGATAGAGATCTG
  GTGTTACAAACGCATCCCCGTGGAAGGCAACTTTGGGCAAACCTATGACTCGGTCAACCAGTCTATCCAT
  GGACTGTCTGGAGAATTTTCAGCCAATATTAACCTTAAGGAAGAGAAGCAGTCTTCAATGGTGGGTCTCA
  ACACCACGAACTGGGAGCACATGGGCACCGAGGTGCGGGACTATGTTCGCCAGCAGGAGACCTATCTGCA
  GTGGGCTATGGGGCTGCTGCACGTGCTTCTGTCCTGCACGTTCCTGCTGGTCTTCCATTCAGCCTTCTCC
  TACATGGACCACTACAACTGGGACATCCGCTTTGACAACATCTACATCAGCACCTACTTCTGTCAGATTG
  ATGCACGCAGGAAGAAGCTGGGCAAACAGAGTCTGTTGCCACTCCGCAAGGCAGAAAGGAAAACGGTTAT
  CTTCCCTTTCAAAGCTACTATCCAGGCCTGGGAAATGCGCTATGTGATAAGAGAGCTCCTAGAGACTCTG
  CCTATTGTGCTGCTGCTCCTGGTGCTGTGCGCGATCGACTGGGCACTCTACTCTGTCTTTGACACCATCC
  GCCAGCACTCTTTCGTGCAGTATTCCTTTCGAAGCAGTCACAAGCTGGAGATAAAAGTCGAGGGAGATTC
  CATACTCGCCAAGCTTCTTCGTAAAACCATCGGCGCTCTGAACACCTCCTCGAGTACAGATGTGGAAACA
  AACAACATGCCCTGCCTGCCCCAACCCATTAGCCTGAATGCCAGGGATTACTTCAAGGCCTCACTGCCAA
  CCCTCCTGCTTGTGTGTCTCTGCCTAGCTCAGGCCTTTGGCTACCGACTCCGGAGAGTCATCGCAGCCTT
  CTATTTCCCCAAGCGAGAGAAGAAGCGGGCTCTGTTCTTCTACAATGAGTTCCTGAAGAAGAGATCAGCT
  TTCACTAAACTGAGGAGGGCCGCCATTGTTCGGAGGGCAAATCAGCAGAAGGCTCCACCCCACTACCTGG
  TGGAGGCTCTGTATCGCCGGTGCCCTCTCCTGCACCGCTTCATGCGTCAGCGCTGTGTGGTGTGCCAGGC
  AATGGAGACGCCGGATTCCTACGTGTGCCCCACACCAGACTGCAAGGCCCTGTACTGCCGGTCGTGCTGG
  GACGACATGCAACGGCTCTGCCCGGTGTGCACGCCCCGCGAAGAGCTCTCGTCATCCGCCCACAGCGACA
  GCAACGACGACGCTGTGTATGGCGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220776 representing NM_029974
  Red=Cloning site Green=Tags(s)
  
  MAFLSSTLHSLGIFEKISRIKEVLKNRLLDLTKRRDQAREQQRKRPHTIIQGLLLWSLPVSWIRFLWRQP
  GEFPVTAFLLGAGTGGLLAIGLFQLLVNPMNIYEEQKVVALYCLASLGAIGWGTSPHIRCASLLLVPKML
  GKEGRLFVMGYALAAIYSGPAANLRSNINEVIASLGCTVELQINNTRSAWRVSTAPLRAVFKGMVGSKDS
  LNKEIQNVSTSFEEMDEQVKSDAGYSSEDWDKNRESTEMFGTSRVRPYLSTQMVYELRTRLRCIHVVNKA
  ILSCYRWFDKKHKNCMRRIHLPLFNNMICVPMKFKFLCNIAKVIEIWCYKRIPVEGNFGQTYDSVNQSIH
  GLSGEFSANINLKEEKQSSMVGLNTTNWEHMGTEVRDYVRQQETYLQWAMGLLHVLLSCTFLLVFHSAFS
  YMDHYNWDIRFDNIYISTYFCQIDARRKKLGKQSLLPLRKAERKTVIFPFKATIQAWEMRYVIRELLETL
  PIVLLLLVLCAIDWALYSVFDTIRQHSFVQYSFRSSHKLEIKVEGDSILAKLLRKTIGALNTSSSTDVET
  NNMPCLPQPISLNARDYFKASLPTLLLVCLCLAQAFGYRLRRVIAAFYFPKREKKRALFFYNEFLKKRSA
  FTKLRRAAIVRRANQQKAPPHYLVEALYRRCPLLHRFMRQRCVVCQAMETPDSYVCPTPDCKALYCRSCW
  DDMQRLCPVCTPREELSSSAHSDSNDDAVYGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029974
• ORF Size: 2196 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029974.2, NP_084250.1
• RefSeq Size: 2312 bp
• RefSeq ORF: 2199 bp
• Locus ID: 77772
• UniProt ID: Q059Y8
• Cytogenetics: 3 F1
• MW: 84.8 kDa
• Gene Summary: This gene encodes a transmembrane protein. A similar gene in human has been associated with dendritic cells. [provided by RefSeq, May 2015]