Sppl2c (NM_199019) Mouse Tagged ORF Clone

CAT#: MR220745

Sppl2c (Myc-DDK-tagged) - Mouse RIKEN cDNA 4933407P14 gene (4933407P14Rik), transcript variant 1

Product Images

Circular map for MR220745

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sppl2c
• Synonyms: 4933407P14Rik; IMP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220745 representing NM_199019
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATGCCTGGGTTCCCTCCACCCCTTGGGCTCACTCCTCCTCCTCTTCCTCCTCCTCCTCCTCAGCC
  CCGAGGCCCGGGGAGAATACGGCCTGGTCCGAGTCGTGTCAAAGAACTGGAGCAAGGACTACTGTGTCCT
  GTACAGCTCTGACTACGTCAACCTCCCCCGGGACCTGCACCATGCCCCACTTCTGTCCCTGCATGATGGC
  ACCAAGACACCCTGGTGTCCAGATGAAGACTCTTTCCACCAGGCCCAGGACAGTTCCCCTAGACAGCGAC
  CCCTGCACCAGACTACTACCATGGTCACGCGTGGCAACTGTAGCTTCTATGCCAAGGGCTGGCTGGCTCA
  AGATCAAGGCGCCCAGGGGTTGCTCATTGTGAGCCGGGCCAGGAATCAACAGTGTTCAGACACCATCTCA
  AAGCCCCAGGACCCCAGCAAGCCCTGGCCTGCCCTCACCATCCCAGTGGCCGTGCTCCGCTACACTGACA
  TGTTGGACATTGTCAGCCACACTTACGGTGACACCGATGTCCGAGTAGCCATGTTTGCACCCCTCGAGCC
  TGTTACTGACTATAACATGGCGATTATCTTCATCTTGGCAGTGGGTACTGTTGCTGCAGGTGGCTACTGG
  GCTGGTTTGATGGAGGCAAACAAGCTGCAGAGGCGCCAGGCCCAAAGAGGAGGGGGGCTTGGTGGTCACA
  ATCAACAACAAACAGTGGCAGCTGAGAGGTCCCAAAGGGCCTGGGAAGATGACGACTTTGAAGATGCACC
  TATGGATTTCACACCAGCCATGACGGGCGCTGTGGTCACCATGTCCTGCTCCATCATGATCCTGCTGTAT
  TTTTTCTATGACTGCTTTGTCTACGTCATGATTGGGATCTTTAGCCTGGGTGCCAGTACCGGCCTCTACA
  GCTGCCTGGCACCCATACTGTGCCACCTGCCCCTATGGCGATACCAGTGGGTCTTGCCTGGACAACGGGT
  TTCTGTAACATGGCCATTACTACTGCTGGCAGGCCTGTGTGCCATGGTGACTGTCCTCTGGGTCATCCAC
  CGAAATGAGGACCATTGGGCATGGCTCCTGCAGGACACTTTGGGTGTCGCCTACTGCCTTTTTGTCCTGA
  GGCGGGTACGGCTGCCCACGTTCAAGAACTGCACTTTGTTCCTGCTGGCCCTACTAGCCTTCGATGTCTT
  CTTTGTCTTTATCACTCCCCTGTTCACCAAGACTGGTGAGAGCATTATGGTAGAAGTGGCATCAGGTCCA
  GCAGATTCTTCAAGCCACGAGAGGTTACCAATGGTGCTCAAGGTGCCCCGGCTAAGCTTTTCCGCCTTGA
  CGTTGTGCAACCAGCCCTTCTCCATCCTTGGCTTTGGGGACATTGTTGTCCCTGGCTTCCTAGTGGCCTA
  CTGTCACCGCTTCGACATGCAAGTCCAATCTCGCCAGGTCTACTATATGGCCTGTACAGTGGCCTATGCC
  GTGGGCCTGCTGGTCACCTTTGTCGCCATGATCCTCATGCAGATGGGCCAGCCTGCCCTGCTCTACTTAG
  TGTCCAGCACTCTGCTCACCAGCCTTGCTGTGGCCACCTGCCGCCAAGAGTTCACCCTTTTCTGGACTGG
  CCAGGGCAGAGCCAAGATTCCTGCTGAGCCTGTGGCACAGCCCTGCATTGCCTCTGCTGTGGGCTCCAAG
  ATGAAGCTGGAGGATGCGAAGGATTCCCGTACAACCAACAGGTTTGAGCAGGCCGTTGATGGAGAGTCAG
  GGGACTTAGAGAGCAGCACTGGGGACGATATGGCAGAGATGGTCACCCTATCTGAGGATGAAGCCACGAG
  TCCAGAGGGCCACAGCGAGAGTTCTGAGGGCTGGAGTGACACCAACCTGGACCCCAATGAGCTACCCTCT
  GGCTCCCCCATGGCCCTAGAGGCCATGTTGATCCCACTGATCCAACCAATCCCACACCCCTCCGAGCTGG
  GCCACATCCGCACTCAATCTCGGGTCCATGATAGCAGCCTGCCCTGGATGGGGTTGCACAAGAGAAAGGG
  CTTGAAGGTGAAGAAGAGTATGTCAGCCCAGGCTCCCCTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220745 representing NM_199019
  Red=Cloning site Green=Tags(s)
  
  MACLGSLHPLGSLLLLFLLLLLSPEARGEYGLVRVVSKNWSKDYCVLYSSDYVNLPRDLHHAPLLSLHDG
  TKTPWCPDEDSFHQAQDSSPRQRPLHQTTTMVTRGNCSFYAKGWLAQDQGAQGLLIVSRARNQQCSDTIS
  KPQDPSKPWPALTIPVAVLRYTDMLDIVSHTYGDTDVRVAMFAPLEPVTDYNMAIIFILAVGTVAAGGYW
  AGLMEANKLQRRQAQRGGGLGGHNQQQTVAAERSQRAWEDDDFEDAPMDFTPAMTGAVVTMSCSIMILLY
  FFYDCFVYVMIGIFSLGASTGLYSCLAPILCHLPLWRYQWVLPGQRVSVTWPLLLLAGLCAMVTVLWVIH
  RNEDHWAWLLQDTLGVAYCLFVLRRVRLPTFKNCTLFLLALLAFDVFFVFITPLFTKTGESIMVEVASGP
  ADSSSHERLPMVLKVPRLSFSALTLCNQPFSILGFGDIVVPGFLVAYCHRFDMQVQSRQVYYMACTVAYA
  VGLLVTFVAMILMQMGQPALLYLVSSTLLTSLAVATCRQEFTLFWTGQGRAKIPAEPVAQPCIASAVGSK
  MKLEDAKDSRTTNRFEQAVDGESGDLESSTGDDMAEMVTLSEDEATSPEGHSESSEGWSDTNLDPNELPS
  GSPMALEAMLIPLIQPIPHPSELGHIRTQSRVHDSSLPWMGLHKRKGLKVKKSMSAQAPL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199019
• ORF Size: 2070 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199019.2, NP_950184.2
• RefSeq Size: 2225 bp
• RefSeq ORF: 2073 bp
• Locus ID: 237958
• UniProt ID: A2A6C4
• Cytogenetics: 11 E1
• MW: 76.7 kDa
• Gene Summary: Intramembrane-cleaving aspartic protease (I-CLiP) that may be able to cleave type II membrane signal peptides in the hydrophobic plane of the membrane.[UniProtKB/Swiss-Prot Function]