Ccdc129 (NM_001081665) Mouse Tagged ORF Clone

CAT#: MR220651

Ccdc129 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 129 (Ccdc129)

Product Images

Circular map for MR220651

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc129
• Synonyms: D530004J12Rik; Gm458
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220651 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAATGGAGGAATTACAAGGATCTGACACTCCCAGAGCAGGTCTGGAGATGAGCAAGAGAGATATCC
  TAAGACATACGAAGAGGGCATGGGCACCTCTGGATGGGCATCTGCTTCCTGACTCTGAGGAAGAAAACCC
  AATGGTCACTATGTATGTGCAAGAAAATTCCAAACAGGAAAGTATCCAGCAGTGGCTAGACTCTGGATTC
  TTTGTCTCTGTAAATGAAAACTTCCAGCAAACCATCAACCATACTGCTTCTTTGCACGAACAAGGAATCA
  TTCAAATGACCGTGAAAGACTACATGAGATCTCTACATCAGTTCTCAGAGACTCCTACCCTGTCCAGAGG
  GACTAGTTTCAACTCGTGTTATTCCACCACAGGTGTACCTCAAAGCATTCCTGAATGGCTGGAATTTTGG
  GAAAAAGATCCAGTGGAGATTCTCTTGGACTTGGGGTTTGGTGCTGATGAACCAGATATCTGCACACAAA
  TCCCAGCCCGGTTCCTTGGCTATAGCTCAGCAGCCAGAGGAATTAACATCCATGTTTTTCTTGAAGCACA
  GAAGCAGCGGATGGACTTTGAGAACCCTGACTTATATGGTCGGTTCCAACAGTTGGAAATCCTGGATCAT
  GTGACCAATGCCTTCTCTTCTTTGCTGGATGGTGTTAAAACCCAACAGAACCAACATGAAGAAAAAGCTG
  AAAGACAGGCTATGCAAAATCCTTCATCAAGTGGAGCTAAAGAACATAAGAGAAAAATGAGCCAACTTTT
  AAAAAGAGCTTCAAGACAGACGAGCAGGATGGATGGTAACCCAGCACTGTCAAAATCATGCAAGACAGAA
  CATGAGATTCCAACTCTGCCAACCAAGCCATGGGATCCTAGAGTGGAGCTACAGGTGGCTTCCATTAGCC
  ATGATGCAAGTCAAATGCTTCCATTAATAGAGTGTGGTTCTGCCCAAGTCCACAATGACTTGTCACCTTG
  TCCTCCTCTTTGTCCTCCTCCGCATGCTCTTCTGGACAAGCCATGGCCTTGCTCATGCACACTGGCCAAG
  CAATCTCCTCATACCTGTTTGTCTGAGGGGTCAGTTAGAAGGAGAAACTGGAAGGAGAAATGGAATCACA
  TGGACAGACTCAAGAACTTGTCACACATTGTGAGTAAAGGACCAGATTCCTTTGAAATGGAAGAGGTCCA
  GAGCTTTGAAGAAGACACTGGCAACCCTCTTATCCTGACATCAGGAATTGTAGGCACTCGAGTGGACAGA
  ACAAACAGCTGTCAGTCTGATAGCAGTGGATTTTTAGAAGAGCTACCAGAGCTCCAGCCCCTCCAGGTGT
  CTTCCATGACAGGCAGCCAGAGTCCTACTGTCTGTAGAGGTTGCAAACCAAGGGATCAGAGTCATAGTCC
  AGCATCACAGCAAGACTCTCTGCAGGAGTCATATGGCTCTAAATCCAAGAGCATGACAAGCTCATCTTTG
  TTGAGCCAAGATTGGAGTACATTGGAAGAAAATGCTTCAGCTTCTGTGGTGGAGGAGGAGCTTCAACTCA
  AGGCCATGGAAGAGCCACCAGAAATAGTCAACCCAGATATGACCCTCATCAAGACTATCATGGTGGGAGA
  ACACCCAGAGAGTGTTGCTGGACCTGTGGTCACTTCTACTTCTACATGTAATAACACAATGGGGGTCTTG
  GTGACTCATGTCACAGAAAAGGAAGATAGGTCTGTAGGCCACAAGGGTACTCGAAAAATGCTGATACAAA
  GGCACCACTTTGAGTCACCAAGGTCATTCAGGATTGATCAGCCTTCAGACAATTTCTATCATGTGGACTC
  TGAGATCACCAAAACAGAGGACAACTGCAAAGTCTGCCCTGATACCAAGCAAAGCTCACTGGTGCAGGAG
  AGGCCAGCACAGCACTCTCACCAGCACAGAGGAGCCATGCCCTATAAAGGCGACCTTGTTCAAACCTCTG
  AAAAGTCAATTCCCCACCTTGATAAGCCACCTGGACATGTTCCCACTGACTCAAATGCTGCCAGCTCTTG
  GTCTGTGACAACCCAGATGTCCTCCAATCTGGTGTCAGCTGCTCAGAGGGTTGCAGACCTAGGGACCTAT
  AATAAAGGAACAGCTTTTGAATGCACTCCATGTGACCCTCTTAATACCACAGACCTAAAACTGCAGACAG
  AAACAAGGCAGGTCAAGGATGTTGCTGTTCAGACTTATGCACATGAATGCAAGCTCAGGCCATCACATAA
  TGACTTCATTCATGGGCCTCGGCCCCTCGCCAAATCCATCTCTTTGGATACAGGTTTTCCTAGTATCTCC
  GCAACAGGCTTCTGCCACACCATACCTGCCCACTGTTGTGTCTGCTGTCATCACTGCCCCAACTGCCAAT
  GGAGGAGGCAAAGCCCTGGCCCAGAACCCTCTATTTGCAGGCATTCCCTGTATTCACAAGCAGAGGATCC
  TAAAGTGCAGTTCATGAAGACATTGAAAATACTCCAAGACACTACTGTGAGGGACCTGTGTTCTTGCACA
  GTCTACGAGATGGAAACCATGAAGATGGTGTGCCAGAGTTTCCGGGAGCACTTAGAGGAAATCGAGCAGC
  ACTTCATGGGACAGCAGGCACTCTATCCCAGGGACATGTCAGAAGAGGAAAGGGAAGAGGCTGAGTACCT
  GAGGACTTTGCGAGAAGCCCTGAGGCAGCAAGTGGCAGAGCTGGCCTTTCAGTTAGGAGACAGAGCCAGG
  CAAATCAAAGAAGGGATCCTACTGCAGCTAGACCTTCTGTGTGAGGAGCTACCTGAACACTGCGACAATC
  TGCCACAATGCAACTGGACAGAAGGAAAGCATGGCCAGAGATCATGTGTTCAAACCCATGCTGTGGCCCC
  TGATCCCACCCTTCCTGCCAGTAGTGGCCAGTGGACTCCCTGCTCTGGGATGACTCAGCCAGTTGCTCTT
  AGTCCCTCTCACCTTGAGACCAGAGGAGGGATATCTCTACAGTCACCAGCTAGGGCAGAATCAGGTCCGG
  GTCCTTCACTCAGTCATGTTGGAGAGAAGTCTCTGTTAGACCAAAGTGGGTTTGATGATCTTTGTACAAA
  ATGCAATGTCCAAGAGCAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220651 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIMEELQGSDTPRAGLEMSKRDILRHTKRAWAPLDGHLLPDSEEENPMVTMYVQENSKQESIQQWLDSGF
  FVSVNENFQQTINHTASLHEQGIIQMTVKDYMRSLHQFSETPTLSRGTSFNSCYSTTGVPQSIPEWLEFW
  EKDPVEILLDLGFGADEPDICTQIPARFLGYSSAARGINIHVFLEAQKQRMDFENPDLYGRFQQLEILDH
  VTNAFSSLLDGVKTQQNQHEEKAERQAMQNPSSSGAKEHKRKMSQLLKRASRQTSRMDGNPALSKSCKTE
  HEIPTLPTKPWDPRVELQVASISHDASQMLPLIECGSAQVHNDLSPCPPLCPPPHALLDKPWPCSCTLAK
  QSPHTCLSEGSVRRRNWKEKWNHMDRLKNLSHIVSKGPDSFEMEEVQSFEEDTGNPLILTSGIVGTRVDR
  TNSCQSDSSGFLEELPELQPLQVSSMTGSQSPTVCRGCKPRDQSHSPASQQDSLQESYGSKSKSMTSSSL
  LSQDWSTLEENASASVVEEELQLKAMEEPPEIVNPDMTLIKTIMVGEHPESVAGPVVTSTSTCNNTMGVL
  VTHVTEKEDRSVGHKGTRKMLIQRHHFESPRSFRIDQPSDNFYHVDSEITKTEDNCKVCPDTKQSSLVQE
  RPAQHSHQHRGAMPYKGDLVQTSEKSIPHLDKPPGHVPTDSNAASSWSVTTQMSSNLVSAAQRVADLGTY
  NKGTAFECTPCDPLNTTDLKLQTETRQVKDVAVQTYAHECKLRPSHNDFIHGPRPLAKSISLDTGFPSIS
  ATGFCHTIPAHCCVCCHHCPNCQWRRQSPGPEPSICRHSLYSQAEDPKVQFMKTLKILQDTTVRDLCSCT
  VYEMETMKMVCQSFREHLEEIEQHFMGQQALYPRDMSEEEREEAEYLRTLREALRQQVAELAFQLGDRAR
  QIKEGILLQLDLLCEELPEHCDNLPQCNWTEGKHGQRSCVQTHAVAPDPTLPASSGQWTPCSGMTQPVAL
  SPSHLETRGGISLQSPARAESGPGPSLSHVGEKSLLDQSGFDDLCTKCNVQEQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081665
• ORF Size: 3105 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081665.1, NP_001075134.1
• RefSeq Size: 3284 bp
• RefSeq ORF: 3105 bp
• Locus ID: 232016
• UniProt ID: Q14B48
• Cytogenetics: 6 B3
• MW: 115.6 kDa