Cd59b (NM_181858) Mouse Tagged ORF Clone

CAT#: MR220268

Cd59b (Myc-DDK-tagged) - Mouse CD59b antigen (Cd59b)

Product Images

Circular map for MR220268

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd59b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220268 representing NM_181858
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGCTCAGAGGGGACTCATCTTACTCCTGCTGCTTCTGGCTGTCTTCTGTTCCACAGCTGTTAGCC
  TCAAATGCTACAACTGTTTAGACCCGGTTTCTTCATGCAAAATAAACACTACTTGCTCTCCTAACCTGGA
  TTCCTGTCTCTATGCTGTAGCCGGAAGGCAAGTGTATCAACAGTGTTGGAAACTTTCGGATTGTAATTCC
  AACTATATTATGAGCCGATTAGACGTGGCAGGCATACAAAGCAAATGCTGTCAGTGGGACTTGTGTAACA
  AAAATCTTGACGGATTAGAAGAGCCAAACAACGCAGAAACTTCCTCATTGAGGAAGACAGCATTGCTGGG
  GACCTCAGTTCTGGTGGCCATTTTGAAGTTTTGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220268 representing NM_181858
  Red=Cloning site Green=Tags(s)
  
  MRAQRGLILLLLLLAVFCSTAVSLKCYNCLDPVSSCKINTTCSPNLDSCLYAVAGRQVYQQCWKLSDCNS
  NYIMSRLDVAGIQSKCCQWDLCNKNLDGLEEPNNAETSSLRKTALLGTSVLVAILKFCF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181858
• ORF Size: 387 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181858.2
• RefSeq Size: 796 bp
• RefSeq ORF: 390 bp
• Locus ID: 333883
• UniProt ID: P58019
• Cytogenetics: 2 E2
• MW: 14.7 kDa
• Gene Summary: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (By similarity).[UniProtKB/Swiss-Prot Function]