Krt85 (NM_016879) Mouse Tagged ORF Clone

CAT#: MR220242

Krt85 (Myc-DDK-tagged) - Mouse keratin 85 (Krt85)

Product Images

Circular map for MR220242

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Krt85
• Synonyms: AA589597; BB007925; Hb5; Krt2-18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220242 representing NM_016879
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTGTCGCTCCTACAGGATCAGCCCAGGATGTGGAGTTACCAGAAACTTCAGCTCCTGCTCAGCCG
  TAGCCCCCAAAACTGGCAACCGCTGCTGCATCAGCGCAGCTCCCTTCCGAGGAGTGTCTTGCTACCGGGG
  ATTGACTGGCTTCAGCAGCCGAAGCCTCTGTAACCCAAGCCCCTGTGGGCCCCGCATGGCTGTTGGGGGC
  TTCCGCTCTGGCTCCTGTGGACGCAGCTTCGGATACCGTTCTGGAGGCGTCTGTGGGCCCAGCCCGCCCT
  GCATCACCACCGTCTCTGTCAATGAGAGCCTGCTCACGCCCCTGAACCTGGAGATCGACCCCAATGCTCA
  GTGTGTGAAGTATGAAGAGAAGGAGCAGATCAAGTGTCTCAACAGCAAGTTCGCGGCCTTCATTGACAAG
  GTGCGATTCCTGGAGCAACAGAACAAGCTGCTGGAGACCAAGTGGCAGTTCTACCAGAACCGCAAGTGCT
  GTGAGAGCAACCTGGAGCCCCTGTTTGGAGGCTACATCGAGGCACTGAGGCGGGAGGCTGAATGTGTGGA
  GGCGGACAGCGGGAGGCTGGCTGCTGAGCTCAACCATGTGCAGGAGGCCATGGAGGGCTACAAGAAAAAG
  TATGAAGAGGAGGTGGCTCTGAGGGCTACAGCAGAGAATGAGTTTGTGGTACTAAAGAAGGATGTGGACT
  GTGCCTACTTACGTAAATCAGACCTGGAGGCCAACGTGGAGGCCTTGGTGGAGGAGTCAAGCTTCCTGAA
  GCGTCTTTATGAAGAGGAGGTCTGTGTTCTGCAAGCCCACATCTCAGACACCTCTGTCATCGTCAAGATG
  GACAACAGCCGAGACCTGAACATGGACTGCGTCGTGGCTGAGATCAAGGCTCAGTATGATGACGTTGCCA
  GCCGCAGCCGTGCTGAGGCTGAGTCCTGGTACCGCACCAAGTGTGAGGAGATGAAGGCCACCGTGATCCG
  GCATGGGGAGACCCTGCGCCGCACGAAGGAGGAGATCAACGAGCTGAACAGAATGATACAGAGGCTGACC
  GCCGAGATCGAGAATGCCAAGTGCCAGCGTGCCAAGCTGGAAGCTGCTGTGGCTGAGGCAGAGCAGCAGG
  GAGAGGCCGCCCTCGCTGATGCCCGCTGCAAGCTGGCTGAGCTGGAGGGTGCCCTGCAGAAGGCCAAGCA
  GGACATGGCCTGCCTGCTCAAGGAGTACCAGGAGGTAATGAACTCCAAGCTGGCACTGGACATCGAGATC
  GCCACCTACAGGCGCCTGCTGGAGGGCGAGGAGCAGAGGCTCTGCGAGGGTGTGGGCTCTGTGAATGTCT
  GTGTCAGCAGCTCCCGTGGAGGAGTCACATGTGGGGGCCTCACCTATGGCACAACCCCAGGGCGCCAGAT
  TGTCTCTGGACCCTCTGTCACTGGGGGCAGCATCACAGTGATGGCCCCTGACTCCTGCTCTCCTTGCCAG
  CCCCGCGCCTCCAGCTTTACCTGTGGGAGCAGCCGCTCAGTGCGCTTCGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220242 representing NM_016879
  Red=Cloning site Green=Tags(s)
  
  MSCRSYRISPGCGVTRNFSSCSAVAPKTGNRCCISAAPFRGVSCYRGLTGFSSRSLCNPSPCGPRMAVGG
  FRSGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKYEEKEQIKCLNSKFAAFIDK
  VRFLEQQNKLLETKWQFYQNRKCCESNLEPLFGGYIEALRREAECVEADSGRLAAELNHVQEAMEGYKKK
  YEEEVALRATAENEFVVLKKDVDCAYLRKSDLEANVEALVEESSFLKRLYEEEVCVLQAHISDTSVIVKM
  DNSRDLNMDCVVAEIKAQYDDVASRSRAEAESWYRTKCEEMKATVIRHGETLRRTKEEINELNRMIQRLT
  AEIENAKCQRAKLEAAVAEAEQQGEAALADARCKLAELEGALQKAKQDMACLLKEYQEVMNSKLALDIEI
  ATYRRLLEGEEQRLCEGVGSVNVCVSSSRGGVTCGGLTYGTTPGRQIVSGPSVTGGSITVMAPDSCSPCQ
  PRASSFTCGSSRSVRFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016879
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016879.2, NP_058575.2
• RefSeq Size: 2594 bp
• RefSeq ORF: 1524 bp
• Locus ID: 53622
• UniProt ID: Q9Z2T6
• Cytogenetics: 15
• MW: 55.8 kDa