Slc14a2 (NM_207651) Mouse Tagged ORF Clone

CAT#: MR220064

Slc14a2 (Myc-DDK-tagged) - Mouse solute carrier family 14 (urea transporter), member 2 (Slc14a2), transcript variant 1

Product Images

Circular map for MR220064

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc14a2
• Synonyms: UT-A1; UT-A3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220064 representing NM_207651
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGACCACCACCCCCTGAAGGAGATGTCTGACAGCAACAGCAGCCCTCTCTTGCCAGAGCCTCTTT
  CCAGCAGATACAAATTGTATGAGTCAGAGCTAAGCAGCCCTACCTGGCCTTCAAGCTCCCAGGATACACA
  CCCAGCCCTTCCCCTGCTGGAAATGCCGGAAGAAAAGGATCTCCGCTCATCAGATGAAGACAGCCACATA
  GTGAAGATTGAAAAACCCAATGAGAGGAATAAAAGGAGAGAAAGTGAGGTGTCCCGCCGGGCTTCTGCGG
  GCCGGGGAGGCTTCAGCCTCTTCCAAGCCGTGAGCTATCTCACTGGCGACATGAAGGAATGCAAGAACTG
  GCTGAAAGATAAGCCCCTGGTCCTACAGTTCCTAGACTGGGTCCTTCGGGGAGCAGCTCAGGTGATGTTT
  GTCAACAACCCCATTAGTGGGCTCATCATCTTCATAGGGCTCCTAATCCAGAATCCCTGGTGGACGATTG
  CTGGGACACTGGGGACAGTGGCTTCAACCTTGGCTGCCCTTGCCTTAAGCCAGGACAGGTCAGCCATCGC
  CTCCGGACTCCATGGCTACAACGGGATGCTGGTGGGACTGCTGATGGCTGTCTTCTCGGAGAAGTTAGAC
  TACTACTGGTGGCTTCTGTTTCCTGTGACCTTCACATCCATGGCCTGCCCCATTATTTCTAGTGCCTTGA
  GTACCATCTTCGCCAAGTGGGACCTGCCCGTCTTCACACTGCCCTTCAACATTGCCTTAACACTGTACCT
  GGCAGCTACGGGCCACTACAACCTTTTCTTCCCCACCACGCTCATCAAGCCTGCGTCTGCAGCTCCCAAT
  ATCACCTGGACAGAGATTGAAATGCCTTTGCTGTTACAAACCATCCCTGTTGGGGTCGGCCAGGTCTACG
  GCTGTGACAACCCCTGGACAGGTGGCATGATCCTGGTGGCTCTGTTTATCTCCTCCCCACTCATCTGCTT
  ACATGCAGCCATAGGGTCAATCGTGGGGCTGCTAGCAGCATTGACTGTGGCCACTCCCTTTGAGACGATC
  TACCTGGGCCTTTGGAGCTACAACTGTGTCCTCTCCTGCATTGCCATTGGCGGCATGTTCTACGCACTCA
  CCTGGCAGACACACCTGCTGGCACTTGTCTGTGCCCTGTTCTGTGCATACATGGGAGCTGCCCTCTCCAA
  TACAATGGCAGTGGTTGGCGTGCCCTCAGGCACCTGGGCCTTCTGTCTCTCCACCCTCACCTTCCTCCTC
  CTCACAAGCAACAACACTGGCATCTACAAGCTCCCACTCAGCAAAGTCACCTACCCGGAGGCCAACCGCA
  TCTACTTCCTGACAGTGAGACGCAGTGAAGAGGAGAAGTCCCCCAATGGTGGCAGTGGAGAGCAGTCACA
  CGGATCAGGCCAGTGGAAGGCAGAAGAGAGCTCTGAGACCGTGCTCCCCAGGCGTAGGAGTGTGTTCCAC
  ATCGAGTGGTCATCCATCCGGAGGAGGAGCAAAGTGTTTGGAAAAGGCGAACACCAAGAGAGGCAAACCA
  AAGAGCCACTGCCCTGCCCGTATCGGAAGCCCACAGTGGAGCTCTTTGACCTGGACACCATGGAGGAGAG
  CACTGAGATAAAGGTGGAAGCCAACACAGCCAGGACTTCCTGGATTCAGAGCTCCATGGTCGCTGGGGGG
  AAGAGAGTCAGCAAAGCCCTCAGCTACATCACAGGAGAGATGAAGGAATGCGGCGAGGGACTTAAAGATA
  AATCTCCAGTGTTCCAGTTTCTTGACTGGGTGCTCCGGGGCATGTCTCAGGTGATGTTTGTAAACAACCC
  TCTCAGCGGCATCCTCATCGTCCTTGGCCTCTTCGTGCAGAATCCCTGGTGGGCTATCTTGGGTTGCTTG
  GGCACTGTCATGTCCACCCTGACTGCCCTCATCCTGAGCCAGGACAAGTCAGCCATTGCAGCAGGACTCC
  ATGGCTACAATGGGGTGCTTGTGGGGCTCCTGATGGCCGTGTTCTCGGACAAGGGCAATTACTACTGGTG
  GCTGCTGCTTCCTGTCATTGTCATGTCCATGACTTGCCCCATCCTTTCCAGTGCCCTGAGCACCGTCTTC
  AGCAAGTGGGACCTCCCAGTCTTCACACTGCCCTTCAACATCGCCGTGACCCTGTACCTGGCAGCGACGG
  GCCACCACAATCTCTTCTTCCCCACGACGCTGCTACAGCCTACAACCACTCCACCCAACATCACCTGGTC
  AGATATCCAAGTGTCTTTGCTTCTGAGAGCCATCCCGGTTGGAATCGGCCAAGTGTACGGGTGTGACAAC
  CCCTGGACTGGAGGCATCTTCCTGGTCGCTCTGTTCATCTCTTCGCCTCTCATTTGCTTGCATGCTGCCA
  TTGGATCCACCATAGGGATGTTAGCAGCACTCAGCATTGCCACACCATTTGACTCCATCTACTTCGGCCT
  GTGTGGCTTCAACAGCACCCTGGCCTGCATCGCCATTGGTGGCATGTTCTACGTCATCACCTGGCAGACC
  CACCTGCTCGCCATTGCCTGTGCCCTGTTTGCAGCCTACCTGGGTGCTGCCCTGGCCAACATGCTGTCTG
  TGTTTGGATTACCACCCTGCACCTGGCCCTTCTGCCTCTCGGCGCTCACCTTCCTCCTCCTGACAAGCAA
  CAACCCTGCCATCTATAAGCTGCCTCTCAGCAAAGTCACCTACCCAGAGGCCAACCGCATCTACTTCCTG
  TCCCAGGAAAAAAACAGAAGGGCATCAACGATAACTAAGTACCAGGCCTACGACGTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220064 representing NM_207651
  Red=Cloning site Green=Tags(s)
  
  MSDHHPLKEMSDSNSSPLLPEPLSSRYKLYESELSSPTWPSSSQDTHPALPLLEMPEEKDLRSSDEDSHI
  VKIEKPNERNKRRESEVSRRASAGRGGFSLFQAVSYLTGDMKECKNWLKDKPLVLQFLDWVLRGAAQVMF
  VNNPISGLIIFIGLLIQNPWWTIAGTLGTVASTLAALALSQDRSAIASGLHGYNGMLVGLLMAVFSEKLD
  YYWWLLFPVTFTSMACPIISSALSTIFAKWDLPVFTLPFNIALTLYLAATGHYNLFFPTTLIKPASAAPN
  ITWTEIEMPLLLQTIPVGVGQVYGCDNPWTGGMILVALFISSPLICLHAAIGSIVGLLAALTVATPFETI
  YLGLWSYNCVLSCIAIGGMFYALTWQTHLLALVCALFCAYMGAALSNTMAVVGVPSGTWAFCLSTLTFLL
  LTSNNTGIYKLPLSKVTYPEANRIYFLTVRRSEEEKSPNGGSGEQSHGSGQWKAEESSETVLPRRRSVFH
  IEWSSIRRRSKVFGKGEHQERQTKEPLPCPYRKPTVELFDLDTMEESTEIKVEANTARTSWIQSSMVAGG
  KRVSKALSYITGEMKECGEGLKDKSPVFQFLDWVLRGMSQVMFVNNPLSGILIVLGLFVQNPWWAILGCL
  GTVMSTLTALILSQDKSAIAAGLHGYNGVLVGLLMAVFSDKGNYYWWLLLPVIVMSMTCPILSSALSTVF
  SKWDLPVFTLPFNIAVTLYLAATGHHNLFFPTTLLQPTTTPPNITWSDIQVSLLLRAIPVGIGQVYGCDN
  PWTGGIFLVALFISSPLICLHAAIGSTIGMLAALSIATPFDSIYFGLCGFNSTLACIAIGGMFYVITWQT
  HLLAIACALFAAYLGAALANMLSVFGLPPCTWPFCLSALTFLLLTSNNPAIYKLPLSKVTYPEANRIYFL
  SQEKNRRASTITKYQAYDVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207651
• ORF Size: 2790 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207651.3, NP_997534.2
• RefSeq Size: 4045 bp
• RefSeq ORF: 2793 bp
• Locus ID: 27411
• Cytogenetics: 18 E3
• MW: 102.6 kDa
• Gene Summary: Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. Vasopressin regulates urea transport by increasing isoform A1 accumulation in the plasma membrane and/or phosphorylation of isoform A1 (By similarity).[UniProtKB/Swiss-Prot Function]