Zcchc4 (NM_030185) Mouse Tagged ORF Clone

CAT#: MR220031

Zcchc4 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 4 (Zcchc4)

Product Images

Circular map for MR220031

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zcchc4
• Synonyms: 4930449I23Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220031 representing NM_030185
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCATGGACTGTCTTGAGTCCCTGGAGGGTGATGGCGACGCGGGGCGTAGGGCGTCCGGGG
  TAGAGGTGGCGCTTCCCTCGAACCCCACGGCTCCTGCCCCGCTGTGCCCGCACGGGCCTACTCTTCTCTT
  TGTAAAAGTGAACCAAGGGAAGGAAGAAACACGAAAGTTTTATGCCTGCTCAGCCTGTAGAGATAGAAAA
  GACTGTAACTTTTTTCAGTGGGAAGATGAAAAGTTGTCAGAAGCTAGACTTGCTGCCCGAGAAATTCATA
  ATCAAAAATGCCAGCCTCCTCTATCCCGGGCACAATGCATAGAAAGGTACCTGAGTTTCATTCAGTTGCC
  CTTGGCTCAAAGAAAGTTCTGTCAAAGTTGCCAGCAGTTGCTGTTACCTGCAGACTGGAGGGAGCACGGC
  ACGCATCAGCTGTCAGCTGACATTTCTGTCGCCCAGCTTGGAAGGCCCAGTCAGCTGCTCTACCCACTGG
  AGAACAAGAAGACGCACGCCCAGTACCTGTTTGCTGACAGGAGCTGCCAGTTTCTGGCAGGCCTCCTTGC
  TACCCTCGGATTCAGCAGAGTGTTGTGTGTTGGAGCACCAAGGCTCCACGAGCAGATCAGGCTAACAGCA
  TCAGGTGAGAGGTCCGGCATGAGGAGCCTTTTATTGGACATTGACTTTCGATATTCACAGTTTTACCTGG
  AAGGAAGCTTTTGCCGCTATAACATGTTCAACCATCATTTCTTTGATGGAAAGGCTGCCCTTGAAGTGTG
  CAAGGAATTCTTACAGGAAGAGGAAGGCAAGGGGGTGATTATGGTGACAGACCCTCCCTTTGGCGGCTTG
  GTGGAACCTCTGGCTATTACATTCAAGAAGTTAATTGCTATGTGGAAAGAAGGTCAAAGCCAAGATGACA
  GCCACAAAGAACTGCCCATTTTCTGGATTTTCCCCTATTTTTTTGAATCACGGATTTGTCAGTTTTTCCC
  GAGCTTCTGCATGCTGGATTACCAGGTGGATTATGATAATCATGCGCTTTACAAACATGGAAAGACAGGT
  CGAAAACAGTCTCCTGTCCGAATTTTCACCAACGTTCCACCCAACAAAATAATCCTTCCATCAGAGGAAG
  GATACAGATTTTGCTCCCTCTGTCAGAGATATGTCTCTCGTGAGAACCAGCACTGTGTGCATTGTAATTC
  TTGCACATCCAAGGACGGCAGGAAATGGAGCCATTGCTTTCTCTGCAAAAAGTGTGTGAAGCCTTCCTGG
  ATCCATTGTAACACCTGCAATCGCTGTGCACTCCCAGACCATTCCTGTTTGGGGCCTAAAGATGGCTGCT
  TTATTTGTGGTGCGCTGGATCACAAACGCAGCAATTGTCCCAACATCGGCACCTCTTGGAGAGCTAACAA
  GTTAGTTGAATTCTTTGGCTTTCTGGAAAAAAGATTGCTCATGTCAAGCTTCCTAGGAGCAATCTACAGA
  AGTACAAAGCTTTCAGAGGACTCAGAATTTTTCAGAGTGCTAAATCGTTCCTCTCACCATTACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220031 representing NM_030185
  Red=Cloning site Green=Tags(s)
  
  MAAPMDCLESLEGDGDAGRRASGVEVALPSNPTAPAPLCPHGPTLLFVKVNQGKEETRKFYACSACRDRK
  DCNFFQWEDEKLSEARLAAREIHNQKCQPPLSRAQCIERYLSFIQLPLAQRKFCQSCQQLLLPADWREHG
  THQLSADISVAQLGRPSQLLYPLENKKTHAQYLFADRSCQFLAGLLATLGFSRVLCVGAPRLHEQIRLTA
  SGERSGMRSLLLDIDFRYSQFYLEGSFCRYNMFNHHFFDGKAALEVCKEFLQEEEGKGVIMVTDPPFGGL
  VEPLAITFKKLIAMWKEGQSQDDSHKELPIFWIFPYFFESRICQFFPSFCMLDYQVDYDNHALYKHGKTG
  RKQSPVRIFTNVPPNKIILPSEEGYRFCSLCQRYVSRENQHCVHCNSCTSKDGRKWSHCFLCKKCVKPSW
  IHCNTCNRCALPDHSCLGPKDGCFICGALDHKRSNCPNIGTSWRANKLVEFFGFLEKRLLMSSFLGAIYR
  STKLSEDSEFFRVLNRSSHHYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030185
• ORF Size: 1536 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030185.3, NP_084461.1
• RefSeq Size: 5756 bp
• RefSeq ORF: 1539 bp
• Locus ID: 78796
• UniProt ID: Q8BKW4
• Cytogenetics: 5 C1
• MW: 58.6 kDa
• Gene Summary: rRNA N6-methyltransferase that specifically methylates the adenine in position 4220 of 28S rRNA. N6-methylation of adenine(4220) in 28S rRNA is required for translation.[UniProtKB/Swiss-Prot Function]