Kctd21 (NM_001039039) Mouse Tagged ORF Clone
CAT#: MR219998
- TrueORF®
Kctd21 (Myc-DDK-tagged) - Mouse potassium channel tetramerisation domain containing 21 (Kctd21)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001039039" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Kctd21 |
Synonyms | EG622320 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219998 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACCCCATTACACTGAATGTTGGGGGGAAGCTCTACACAACCTCACTGGCAACCCTGACCAGCT TCCCCGACTCCATGCTGGGGGCCATGTTCAGTGGGAAGATGCCCACCAAGAGGGACAGCCAGGGTAACTG CTTCATTGACCGTGATGGCAAAGTGTTCCGCTATATCCTCAACTTCCTGCGGACCTCTCACCTGGATCTG CCAGAGGACTTCCAGGAGATGGGTCTGCTGCGCAGGGAAGCCGACTTCTACCAGGTACAGCCCCTGATCG AGGCCCTGCAGGAAAAAGAGGTAGAACTCTCCAAGGCGGAGAAGAATGCCATGCTTAACATCACCCTGAA GCAGCGCGTGCAGACGGTCCACTTCACCGTGCGGGAGGCACCACAGATCTACAGCCTGTCGTCTTCCAGC ATGGAGGTGTTCAACGCCAACATCTTCAGCACGTCCTGCCTCTTCCTCAAACTCCTTGGCTCTAAGCTCT TCTACTGCTCCAATGGCAATCTCTCCTCCATCACCAGCCACTTGCAGGACCCTAACCATCTGACTCTTGA CTGGGTAGCCAATGTGGAAGGCCTTCCAGAGGAAGAGTACACCAAGCAGAACCTGAAAAGGCTGTGGGTG GTCCCTGCCAACAAGCAGATCAACAGCTTCCAGGTCTTTGTGGAGGAGGTGCTGAAGATCGCTCTGAGTG ATGGGTTCTGCATTGACTCTTCCCACCCACACGCTCTGGACTTCATGAACAATAAGATCATTCGGTTAAT CCGGTACAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219998 protein sequence
Red=Cloning site Green=Tags(s) MSDPITLNVGGKLYTTSLATLTSFPDSMLGAMFSGKMPTKRDSQGNCFIDRDGKVFRYILNFLRTSHLDL PEDFQEMGLLRREADFYQVQPLIEALQEKEVELSKAEKNAMLNITLKQRVQTVHFTVREAPQIYSLSSSS MEVFNANIFSTSCLFLKLLGSKLFYCSNGNLSSITSHLQDPNHLTLDWVANVEGLPEEEYTKQNLKRLWV VPANKQINSFQVFVEEVLKIALSDGFCIDSSHPHALDFMNNKIIRLIRYR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001039039 |
ORF Size | 783 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001039039.3, NP_001034128.2 |
RefSeq Size | 2975 bp |
RefSeq ORF | 783 bp |
Locus ID | 622320 |
UniProt ID | Q3URF8 |
Cytogenetics | 7 E1 |
MW | 29.7 kDa |
Gene Summary | Probable substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes the ubiquitination of HDAC1. Can function as antagonist of the Hedgehog pathway by affecting the nuclear transfer of transcription factor GLI1; the function probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. Inhibits cell growth and tumorigenicity of medulloblastoma (MDB).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215212 | Kctd21 (untagged) - Mouse potassium channel tetramerisation domain containing 21 (Kctd21), (10ug) |
USD 330.00 |
|
MG219998 | Kctd21 (tGFP-tagged) - Mouse potassium channel tetramerisation domain containing 21 (Kctd21), (10ug) |
USD 500.00 |
|
MR219998L3 | Lenti ORF clone of Kctd21 (Myc-DDK-tagged) - Mouse potassium channel tetramerisation domain containing 21 (Kctd21) |
USD 600.00 |
|
MR219998L4 | Lenti ORF clone of Kctd21 (mGFP-tagged) - Mouse potassium channel tetramerisation domain containing 21 (Kctd21) |
USD 600.00 |
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