Alpk1 (NM_027808) Mouse Tagged ORF Clone

CAT#: MR218938

Alpk1 (Myc-DDK-tagged) - Mouse alpha-kinase 1 (Alpk1)

Product Images

Circular map for MR218938

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alpk1
• Synonyms: 8430410J10Rik; LAK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218938 representing NM_027808
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAATCAAGATGCCGTGGCTTCGATACTGCATGAGTGCAAGCAAGTGCTGGATCGTCTCTTGTTGG
  AAACGCCAGATGTGTCTACAGAGGACAAGAGTGAGGACCAGCGGTGCAGAGCTTCACTCCCCAGCGAGTT
  AAGGACCCTGATCCAGGAGGCAGAGGAAATGAAGTGGCCCTTCGTGCCTGAAAAGTGGCAATACAAACAA
  GCCATGAGCCCAGAGGACAAAACAAACCTGCAAGATGTGATTGGCGCCGGGCTGCAGCAGTTACTGGCGG
  CCTTGCGAGCCTCCATCCTTGTCCAGGACTGCGCTGCCGCCTCCGCTATTGTCTTCTTGATGGACCGGTT
  CCTGTATGGACTCGACGTCTCCGGAAAACTCCTGCAGGTCGCCAAAGGCTTGCACAAGCTGAAGCCTGCA
  ACGCCCATCGCCCCGCAGGTGGTCATCCGACAAGCCCGAGTCTCCGTCAACTCAGGGAAACTGTTGAAGG
  CAGAATACATCCTGAGCAGCCTGATAAGCAACAACGGAGCAACCGGGACCTGGCTGTATAGGAATGAGAG
  TGACAAAGTCCTGGTACAGTCGGTCTGCATACAGATCAGAGGGCAGATTCTGCAGAAGCTAGGCATGTGG
  TATGAAGCGGCAGAATTGATATGGGCCTCTGTCATAGGATATCTGACACTCCCTCAGCCAGATAAGAAGG
  GCATTTCCACATCACTGGGCATACTGGCAGACATCTTTGTTTCCATGAGCAAAACCGATTATGAAAAGTT
  TAAGAAGAGCCCAAAGGTTAACTTGGCCCTGCTCAAGGAGTTTGACCACCATTTGTTGTCAGCCTCGGAA
  GCCTGCAAGCTGGCAGCTGCCTTCAGTGCCTACACACCCCTCTTTGTGCTCACGGCTGTGAATATCCGTG
  GCACATGTTTGTTGTCATATAGCTGTTCTGCTGACTGCCCTCCGGGAATGAAAAGTGTGCATCTGTGTGA
  AGCCAAAGAAGCTTTTGAGATTGGCCTCCTGACCAAGAAAGATGGCGAGCTGGTTAGTGGCAAGCAGGAG
  CTGCACAGTTTCATCAAAGCTGCTTTCGGCCTCACCACGGTGCACTCCAGACTCCATGGGGAGACAGATG
  CAGTTCGCGCAGCAAGGCAGCTATGCAGTGAAGCTGTGGGCAAGCTCTACACCTTCAGCACTTCCCCCAC
  AAGCCAGGACAGAGAAGGCCTGTCTCAGGAAATCATGTCTCTCATCAGCCAGGTGAAGGGACATCTACGA
  GTTCAAAGCTTCCCAAATTTAGATGCTTGCTCTTATGTCCCAGAGAGTTTCAAGTGTGGTTTAGATCGAC
  TCATCTTGCATGGACACGTGGATTTCCAACAAATTCTTGAAACCTATTCCCAGCACCATACTTCAGTGTG
  TGAGGTATTTGAAAGCACTTGTGGGAACAGCAAACGCAACCAGAGAGACACAAAATCGGAGGTCTGTATT
  ACCACTCTGAAGACAGAAACAAACACCGCAGATACTATGGTTGCTACTCTAGAGAGGGTGAGTTCCCAAG
  ACAGCAGGAGTACAGCTTCTTCCAAAATGTCTAAGAAAGATCAAGGGAAGCTCCAAAGAGAAAGGGGGAG
  AAGCTGGACCCATTCCAAGGCATTCCGAGTCTCCCTGGATCTAGATATGGAGACTGAGACTGAGCCACCA
  AATCACAGCAATGGAGGGACAGATGTTTTCAACAAGTCTCTGAGAGACAACTCTAGCTCCTGTTCTTGGG
  GCAGACTGTCAGGGCTCAGCTCTTCCACAAGCTGGGAGGAAGTGAACTGCGCTGTTCAGGATGTAGTAAG
  GAAAGGGTCTGGCCAAGAAGAGCATCCTGTGGAGGCGCAAAGCTCTGAGGCAGCGTCTGAGGAGCCCAAG
  AGGAACAGAAGCAGCAGAGCTGTGTATTTGTCTTCAAAGCTCCGTGGTGTCTCTCTTCAGACAACTGGAG
  ATGACAACTTAGAGTCTTCACCAAGTCAGTTACACAATCACACATCCATCCTTCCTTTCAATGCAAAAGA
  TACTTGTTTGGCCTCGGGTGCAGGACTGGTAGAAACCGCTGAAGGATCTAGCAATACTTCTCTTCAGTCC
  AGTCATTCGTGTGGTTCTGATTCCTGGTCCTTGTCTAGTTCAGACAGGTTCACGGATGTGACCACGAATC
  CTTCAGTTCAGGAAGAAGAACCCTCTGGAATAATGGGTGATGTCCCAGAATCCAAGTATGACTGTAAAGA
  CTGGCACGGAGAAAAGAATGGGGGAACACTCGCTGAAATATGCACAGGGCCTGAATTAACATCTGCCCCC
  TCTTCGGTCGACCCAGAAGGAGAAACAGCAGAAAGCACAGACGATGGACTATCACCCTCTCAGGTAGCCC
  TGGGATGTTTAGAAGGCAGCCATTCAATGTCTACGCGTAGGACTTTCTTTCCGGATGGGTCGGTTCAAAA
  CGCTGACTCAGCAAAGACTGGCTGCTCAGTCAGAGACCAGACTGTCGACCCTGATGCCTCAACGGTGGAT
  GAGGAGGGCCAAATGCTTGACAGCACAGAGATCTGCTCCATTGGCCAAGATGGCGCTCACAGACCACGTG
  CTTTGAGGTCCGGTCAGAGTGCGGAGGGGCCAAAGTCCTTTGTAAATGGCTCCAGTCCTTCCCCTATCTT
  CGATGAGGACTTCAGCACCACAGAGGAAGGAGAGGAGCTTGGGAGCATGCTGAAGAGCAGCCAAAACTCC
  AGCTCATACTCACCATGGTGGCTAAAATCACCAGCCTTTTCCCGCAGTTCGTCAGATGGGGAAAGCTCCT
  GGTCTCTGCTGAACTCAAGCAGAAGTTCCTTCGCCTCGCTGGCAGGACAGACCAGTCAAGAGATCCTTGA
  GGCTCGAACCCTACAGCCTGATGACCTTGAAAAACTTCTGGCGGGCGTGAGGCATGATTGGCTGCTTCAG
  AGACTGGAGAATACTGGCGTTTTGAAGTCCAATCAACTCCAACAAGCCCACAGTGCCCTTCTGCTAAAAT
  ATTCCAAAAAGTCTGAGTTGTGGACAGCGCAGGAAACTGTGGTGTATCTGGGAGACTACCTGAAAGTGAA
  GAAGAAGGGCAAGCAGAGAAATGCATTTTGGGTCCACTATCTCCATCAAGAGGAAACCCTGGGGAGATAC
  GTTGGGAAAGAATACAAAGAGAGGAAGGGGCTCCGGCACCACTTCACTGATGTGGAGCGGCAGATGACAG
  CACAGCACTACGTGACAGAGTTTAACAAGAGACTCTACGAGCAAAAGATTCCGACTCAGATATTCTACAT
  CCCCTCTACAATATTACTGATTTTGGAAGACAGGACTATAAAGGGATGCATCAGTGTAGAGCCTTACATC
  CTGGGGGAATTCGTCAAGTTATCCAATAACACCAAAGCAGTCAAAAATGAGTACAAAGCTACAGAATATG
  GCTTGGCCTATGGCCATTTTTCTTATGAGTTTTCTAACCATAGAGATGTGGTGGTTGATTTACAAGGTTG
  GGTGACTGGTAATGGAAAAGGCCTGATCTATCTCACGGATCCTCAGATTCACTCTGTAGACCAGAAAGAT
  GTCACTACAAACTTTGGAAAGCGGGGAATATTTTACTTCTTTAATAACCAACATGCAAGCTGTAATGAAA
  TATGCCATCGTCTTTCTCTGACTAGACCTTCACTAGAGCAAACAAGTAAGGTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218938 representing NM_027808
  Red=Cloning site Green=Tags(s)
  
  MNNQDAVASILHECKQVLDRLLLETPDVSTEDKSEDQRCRASLPSELRTLIQEAEEMKWPFVPEKWQYKQ
  AMSPEDKTNLQDVIGAGLQQLLAALRASILVQDCAAASAIVFLMDRFLYGLDVSGKLLQVAKGLHKLKPA
  TPIAPQVVIRQARVSVNSGKLLKAEYILSSLISNNGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMW
  YEAAELIWASVIGYLTLPQPDKKGISTSLGILADIFVSMSKTDYEKFKKSPKVNLALLKEFDHHLLSASE
  ACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSCSADCPPGMKSVHLCEAKEAFEIGLLTKKDGELVSGKQE
  LHSFIKAAFGLTTVHSRLHGETDAVRAARQLCSEAVGKLYTFSTSPTSQDREGLSQEIMSLISQVKGHLR
  VQSFPNLDACSYVPESFKCGLDRLILHGHVDFQQILETYSQHHTSVCEVFESTCGNSKRNQRDTKSEVCI
  TTLKTETNTADTMVATLERVSSQDSRSTASSKMSKKDQGKLQRERGRSWTHSKAFRVSLDLDMETETEPP
  NHSNGGTDVFNKSLRDNSSSCSWGRLSGLSSSTSWEEVNCAVQDVVRKGSGQEEHPVEAQSSEAASEEPK
  RNRSSRAVYLSSKLRGVSLQTTGDDNLESSPSQLHNHTSILPFNAKDTCLASGAGLVETAEGSSNTSLQS
  SHSCGSDSWSLSSSDRFTDVTTNPSVQEEEPSGIMGDVPESKYDCKDWHGEKNGGTLAEICTGPELTSAP
  SSVDPEGETAESTDDGLSPSQVALGCLEGSHSMSTRRTFFPDGSVQNADSAKTGCSVRDQTVDPDASTVD
  EEGQMLDSTEICSIGQDGAHRPRALRSGQSAEGPKSFVNGSSPSPIFDEDFSTTEEGEELGSMLKSSQNS
  SSYSPWWLKSPAFSRSSSDGESSWSLLNSSRSSFASLAGQTSQEILEARTLQPDDLEKLLAGVRHDWLLQ
  RLENTGVLKSNQLQQAHSALLLKYSKKSELWTAQETVVYLGDYLKVKKKGKQRNAFWVHYLHQEETLGRY
  VGKEYKERKGLRHHFTDVERQMTAQHYVTEFNKRLYEQKIPTQIFYIPSTILLILEDRTIKGCISVEPYI
  LGEFVKLSNNTKAVKNEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGLIYLTDPQIHSVDQKD
  VTTNFGKRGIFYFFNNQHASCNEICHRLSLTRPSLEQTSKV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027808
• ORF Size: 3693 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027808.1, NP_082084.1
• RefSeq Size: 4795 bp
• RefSeq ORF: 3696 bp
• Locus ID: 71481
• Cytogenetics: 3 G2
• MW: 136.3 kDa