Zcchc11 (NM_175472) Mouse Tagged ORF Clone

CAT#: MR218919

Zcchc11 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 11 (Zcchc11)

Product Images

Circular map for MR218919

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zcchc11
• Synonyms: 6030404K05Rik; 9230115F04Rik; mKIAA0191; PPAPD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218919 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCCCAAGACTTCTAAAAACGAAAATCATGAACCAAAGAAGAATATTATTTGTGAAGAAAGCA
  AAGCAGTTAAAATTATATCTAATCAAACCTTGAAGCCTAGAAATGATAAGTCAGAAATTGGGACCAGCTC
  TCTGAATAGAAATAGTAGTAAAAAAACCAAGCAAAATGATATTTGTATAGAAAAAACAGAAGCTAAATCA
  TGTAAAGTAAACGCTGCCAGTGTTCCAGGCCCTAAAGATTTGGGGTTAGTACATCGAGATCAAAGTCATT
  GCAAAATGAAAAAGTTACCAAATTCACCCATGAAAGCCCAGAAGGGATCTTCACAGACAAAATTAGAAAA
  GACACCTAGTTTACAGACAAAAGCAGAAAAAGTACCAAAGTCTCCAAATTTACCAGTAAAAGCAGAAAAA
  GCGCCCTGTACAACAGCAGAAGCAACAACAGAAAAAGCACTTAATTCACAGAGAAAAGAAGAAAACACAC
  CCACTTCTCAGATGAAATTACAGAAGACACCCAGATCACCTTTAGAGCCAGAAAATGTGCCCAGTTTACT
  ATTGAAAGAAAATGTGAAACAGACAGAATCACAACAGACTGGGAAGAAACTTACAAGCTCCTTTGTTTCT
  ATGGATAAAAGGAAGAGTGAAGCTCTACAGGGAGAAAAGTCAGCTCTGGAAAACTCATCACTATCTCAGA
  AGCAGCAGACTCAGACAGATAATATTGCTGATTCTGATGACAGTGCTTCAGGGATTGAAGATACAGCAGA
  TGATTTGAGTAAAATGAAGAGTGAGGAATCTAACAAAGAAAATTCTTCTGAGATGGACTATTTAGAAAAT
  GCCACTGTAATAGATGAATCTGCTTTGACACCTGAGCAGAGGCTAGGCTTGAAACAAGCAGAAGAACGTC
  TAGAAAGAGATCACATCTTCCGACTTGAAAAACGGTCACCAGAATATACCAATTGCCGCTATCTATGCAA
  ACTTTGCTTAATTCACATTGAAAACATCCAAGGAGCACATAAACATATAAAAGAAAAACGACATAAGAAA
  AATATTTTGGAAAAACAAGAAGAGAGTGAACTTCGGTCTCTTCCATCTCCTTCCTCTGCTCACTTGGCTG
  CTTTAAGTGTTGCAGTTGTTGAGTTAGCAAAAGAACAAGGAATAACAGATGATGACCTCAGAATCCGCCA
  GGACATTGTGGAGGAAATGTCAAAGGTTATAATGACATTTTTACCAGAATGTTCACTGAGGTTGTATGGT
  TCATCTCTGACAAAGTTTGCTCTGAAAAGCAGTGATGTTAATATAGATATAAAATTTCCTCCCAAGATGA
  ATCATCCAGATCTTCTGATACAAGTGCTTGGAATTCTGAAAAAAAGTGCATTATATATAGATGTGGAATC
  TGATTTTCATGCTAAAGTTCCTGTTGTGGTGTGCAAAGATCGGAAAAGTGCTTTACTGTGTAGAGTAAGT
  GCAGGAAATGATATGGCGTGTCTCACAACTGACTTACTTGCTGCTCTTGGCAAAGTGGAACCCGTCTTCA
  CTCCCTTAGTGCTAGCTTTTCGCTATTGGGCTAAGCTGTGCTATATTGACTCCCAAACTGATGGTGGAAT
  CCCTTCTTACTGCTTTGCTTTGATGGTGATGTTTTTTCTTCAACAAAGAAAACCCCCTCTTCTTCCTTGC
  TTACTTGGAAGTTGGATTGAAGGCTTTGACCCGAAAAGAATGGATGACTTTCAGCTGAAGGGCATAGTAG
  AAGAGAAGTTTGTGAAGTGGGAATATAATTCAAGTAGTGCAACTGAGAAAAACTTAATTGCTGATGAAAA
  CAAAGCTAAGGCAGACGAACCAAAAGATGATACCAAGAAGACAGAAACAGACAACCAAAGTAATGCTGCG
  AAGGCAAAACATGGCAAATCTCCTTTAACATTGGAAGCACCAAATCAGGTACCCTTGGGACAGTTGTGGT
  TGGAGCTGCTAAAATTTTACACACTGGATTTTGCTTTGGAGGAATATGTCATATGTGTACGGATACAAGA
  TATTCTAACTAGAGAGAACAAAAACTGGCCTAAAAGGCGGATAGCCATTGAAGATCCATTTTCAGTCAAG
  AGGAATGTTGCCCGGAGCTTAAACAGCCAGCTTGTATACGAATATGTTGTGGAAAGATTCAGAGCAGCTT
  ATCGATATTTTGCCTGTCCTCAAAAGAAGGGTGGAAATAAATCTACAATGGATCCTAAGAAGAAAGAGAA
  GGGGAAGCTAAGCAGTAAGAAACCAGTGAAGTCTGATTGTTCGGCAACCAATTGTTGCATTCTTGGGGAA
  AGTGCAGAAAAAATACATATGGAAAGAGGCCAACCTGCTAAACATGATGAAACGGAGTTTACATCACAGA
  GATGTATTGTTGATAATGACAGTTTATTGGTAAATGAACTAGGTTTGGCTAACCATGGACAAGCTTCTTC
  ATCTCTTTCTACTGCCAGTGGAGGCAGTGACTTAAAGCAAAAATCAGCTGAGAAACAAGGTGATTTAACA
  CCTTCAGAAACTTCCTTAAAGAAGGAACTCAGCCAGTGTATTTGCATTGGGACCCCTGATGGAGCTGAAT
  CTGCTGGAACAGACTGCAGATCAAACTTAGAAATGGACAGTTCACATCAGATTGTGTGCAACAATGTATC
  TGCTACCTCTTGCAACTGCAAAGCTACAGAAGTTACTTCTGACCTTGTTGATGAGGATAACCTCCCCTCC
  CAGGAATTATATTATGTGTTTGATAAGTTCATTTTAACCTCTGGCAAGCCGCCAACAATAGTATGCAGCA
  TCTGCAAAAAGGATGGCCATTCAAAAAATGATTGTCCAGAGGATTTCAGGAAAATTGATCTAAAACCTCT
  ACCACCAATGACAAACCGATTTCGAGAAATACTTGATTTAGTATGTAAAAGATGTTTTGATGAATTATCA
  CCGCCTTGCTCTGAACAACACAACAGGGAACAAATTTTAATTGGCTTGGAAAAGTTTATTCAAAAAGAAT
  ATGATGAAAAAGCAAGATTGTGCTTATTTGGTTCTTCTAAGAATGGATTTGGATTCCGAGATAGTGATCT
  GGATATTTGTATGACTCTGGAAGGCCATGAAAATGCAGAGAAATTAAACTGTAAAGAAATAATTGAAAAT
  TTGGCGAAAATTCTTAAGAGGCATCCAGGTTTAAGAAACATTCTGCCTATAACTACTGCCAAAGTGCCTA
  TTGTAAAATTTGAACACAGGAGAAGTGGACTAGAAGGTGATATCAGTTTATATAACACATTGGCTCAACA
  CAACACAAGAATGCTAGCTACTTATGCAGCTATTGATCCTAGAGTACAGTACTTGGGATATACTATGAAA
  GTGTTTGCAAAGCGCTGTGACATTGGAGATGCATCTCGGGGAAGTTTATCTTCATATGCTTACATCCTTA
  TGGTGCTGTACTTTCTGCAGCAAAGAAAGCCACCAGTTATCCCAGTTCTACAAGAGATCTTTGATGGGAA
  GCAGATTCCGCAGAGAATGGTTGATGGATGGAATGCCTTTTTCTTTGATAAAACAGAAGAGCTGAAAAAG
  CGTTTACCTTCACTTGGGAAGAACACGGAATCACTGGGAGAGCTTTGGTTAGGACTGCTTCGCTTCTATA
  CAGAAGAGTTTGATTTTAAGGAGTATGTAATCAGTATTCGGCAGAAGAAGCTGCTGACCACTTTTGAAAA
  GCAGTGGACTTCTAAATGCATTGCAATTGAAGATCCTTTTGACTTGAATCATAACCTTGGTGCTGGTGTT
  TCTAGAAAAATGACTAATTTTATTATGAAGGCGTTTATCAATGGAAGGAAACTTTTTGGTACCCCCTTTT
  ATCCACTCATTGGCAGAGAAGCTGAGTACTTCTTTGATTCAAGAGTACTAACAGATGGAGAGTTGGCTCC
  TAATGATCGTTGTTGCCGTGTGTGTGGAAAAATAGGTCACTACATGAAAGACTGTCCCAAAAGGAAAAGA
  CTAAAGAAGAAAGACAGTGAAGAAGAGAAGGAAGGGAACGAAGAAGAAAAAGACTCCCGTGACCTTCTTG
  ACTCCCGAGACCTTAGGTGTTTCATATGTGGAGATGCTGGACATGTTCGCCGCGAATGCCCAGAGGTCAA
  GATGGCCCGCCAGAGGAATAGTAGTGTGGCAGCAGCCCAGCTGGTCCGTAACCTTGTAAATGCTCAGCAG
  GTGGCTGGTTCAGCGCAGCAACAGAGTGACCAGTCCATAAGGACCAGACAGTCTTCAGAATGTTCTGATT
  CACCATCTTATTCTCCTCAGCCTCAGCCTTTTCCACAGAATTCTCCCCAACCATCTGCTCTTCCCCCACC
  TCCATCCCAGCCAGGATCCCAGCCTAAGCTTGGCCCACCCCAGCAGGGAGGGCAGCCCCCTCATCAGGTT
  CAGATGCCCTTGTATAACTTTCCTCAGTCACCTCCAGCTCATTATTCACCCATGCACAGTATGGGGTTAC
  TGCCAATGCACCCCCTGCAGATCCCTGCCCCATCGTGGCCCATCCACGGCCCGATGCTGCACTCTGCACC
  TGGCAGCACCCCCAGCAACATTGGCTTGAATGATCCCAGCATCATCTTTGCACAGCCTGCTGCCAGGCCC
  ATGGCAATTCCCAGCCCTTCTCACGATGGACACTGGCCTCGGACCGTGGCTCCCAATTCCCTGGTGAACA
  ATGGTGCAGTGGGTAATTCGGAGCCACGCTTTCGAGGACTAAATCCTCCAATTCCATGGGAACATGCACC
  ACGCCATTTCCCCCTTGTCCCAGCTTCGTGGCCTTACGGTTTGCATCAAAATTTCATGCATCAGGGAAAT
  CCAAGATTTCAGCCCAAACCCTTCTATGCTCAAGCAGACAGATGTGCCACGCGTCGCTGTAGAGAGCGCT
  GTCCCCACCCACCACGAGGAAATGTGTCCGAG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218919 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEPKTSKNENHEPKKNIICEESKAVKIISNQTLKPRNDKSEIGTSSLNRNSSKKTKQNDICIEKTEAKS
  CKVNAASVPGPKDLGLVHRDQSHCKMKKLPNSPMKAQKGSSQTKLEKTPSLQTKAEKVPKSPNLPVKAEK
  APCTTAEATTEKALNSQRKEENTPTSQMKLQKTPRSPLEPENVPSLLLKENVKQTESQQTGKKLTSSFVS
  MDKRKSEALQGEKSALENSSLSQKQQTQTDNIADSDDSASGIEDTADDLSKMKSEESNKENSSEMDYLEN
  ATVIDESALTPEQRLGLKQAEERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKK
  NILEKQEESELRSLPSPSSAHLAALSVAVVELAKEQGITDDDLRIRQDIVEEMSKVIMTFLPECSLRLYG
  SSLTKFALKSSDVNIDIKFPPKMNHPDLLIQVLGILKKSALYIDVESDFHAKVPVVVCKDRKSALLCRVS
  AGNDMACLTTDLLAALGKVEPVFTPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPC
  LLGSWIEGFDPKRMDDFQLKGIVEEKFVKWEYNSSSATEKNLIADENKAKADEPKDDTKKTETDNQSNAA
  KAKHGKSPLTLEAPNQVPLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVK
  RNVARSLNSQLVYEYVVERFRAAYRYFACPQKKGGNKSTMDPKKKEKGKLSSKKPVKSDCSATNCCILGE
  SAEKIHMERGQPAKHDETEFTSQRCIVDNDSLLVNELGLANHGQASSSLSTASGGSDLKQKSAEKQGDLT
  PSETSLKKELSQCICIGTPDGAESAGTDCRSNLEMDSSHQIVCNNVSATSCNCKATEVTSDLVDEDNLPS
  QELYYVFDKFILTSGKPPTIVCSICKKDGHSKNDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELS
  PPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEIIEN
  LAKILKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMK
  VFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEIFDGKQIPQRMVDGWNAFFFDKTEELKK
  RLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGV
  SRKMTNFIMKAFINGRKLFGTPFYPLIGREAEYFFDSRVLTDGELAPNDRCCRVCGKIGHYMKDCPKRKR
  LKKKDSEEEKEGNEEEKDSRDLLDSRDLRCFICGDAGHVRRECPEVKMARQRNSSVAAAQLVRNLVNAQQ
  VAGSAQQQSDQSIRTRQSSECSDSPSYSPQPQPFPQNSPQPSALPPPPSQPGSQPKLGPPQQGGQPPHQV
  QMPLYNFPQSPPAHYSPMHSMGLLPMHPLQIPAPSWPIHGPMLHSAPGSTPSNIGLNDPSIIFAQPAARP
  MAIPSPSHDGHWPRTVAPNSLVNNGAVGNSEPRFRGLNPPIPWEHAPRHFPLVPASWPYGLHQNFMHQGN
  PRFQPKPFYAQADRCATRRCRERCPHPPRGNVSE
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175472
• ORF Size: 4935 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175472.3, NP_780681.2
• RefSeq Size: 6054 bp
• RefSeq ORF: 4935 bp
• Locus ID: 230594
• UniProt ID: B2RX14
• Cytogenetics: 4 C7
• MW: 184.6 kDa
• Gene Summary: Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:28792939). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (PubMed:28792939). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (By similarity). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency (By similarity). Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (PubMed:19703396). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (By similarity). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP (By similarity). Does not play a role in replication-dependent histone mRNA degradation (By similarity). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:28671666, PubMed:28792939, PubMed:22898984). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (By similarity).[UniProtKB/Swiss-Prot Function]