Atg10 (NM_025770) Mouse Tagged ORF Clone
CAT#: MR218897
- TrueORF®
Atg10 (Myc-DDK-tagged) - Mouse autophagy-related 10 (yeast) (Atg10)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_025770" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Atg10 |
Synonyms | 5330424L23Rik; 5430428K15Rik; Agp10; AI852123; APG10; Apg10l; Apg10p; Atg10l |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR218897 representing NM_025770
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATGAGTTCTTTGGAGAAAAAAGCTTCCAGCATTATTGTGCAGAATTCATCAGACATTCACAGC AGATAGGCGATGGCTGGGAATGGAGAACAGCCAAGGAATGTTCTGATGGCTACATGTGCAAAACACAGTT TCGAATAAAGAATGAGACCTTGACACCACATGCCAGTGTGCTCACATGTCTCCCTACAGAGGAGAATTTA GAGCTACCAATGGATGATTCTGAAGTGACGAGACCTGCAGCGGTGGCAGAAGTGATTAAACATGAGTATC ATGTCTTATACTCTTGTAGTTACCAAGTGCCGGTTCTTTACTTTAGGGCAAGCTTTTTAGATGGGAGACC GTTAGCTCTGGAGGATATATGGGAAGGTGTTCATGAGTGCTATAAGCCGAGGCTGCTCCAGGGACCGTGG GATACCATCACCCAGCAGGAACATCCAATACTTGGGCAACCATTTTTTGTTCTACATCCCTGCAAGACAA ATGAATTCATGACTGCTGTGCTAAAGAATTCACAGAAAATCAATAGAAATGTCAACTATATCACATCATG GCTGAGCCTCGTGGGGCCAGTGGTTGGTCTGAATTTACCTCTGAGCTATGCTAAAGCAACATCACAATCG GAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218897 representing NM_025770
Red=Cloning site Green=Tags(s) MEDEFFGEKSFQHYCAEFIRHSQQIGDGWEWRTAKECSDGYMCKTQFRIKNETLTPHASVLTCLPTEENL ELPMDDSEVTRPAAVAEVIKHEYHVLYSCSYQVPVLYFRASFLDGRPLALEDIWEGVHECYKPRLLQGPW DTITQQEHPILGQPFFVLHPCKTNEFMTAVLKNSQKINRNVNYITSWLSLVGPVVGLNLPLSYAKATSQS E TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025770 |
ORF Size | 633 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025770.3, NP_080046.3 |
RefSeq Size | 1609 bp |
RefSeq ORF | 636 bp |
Locus ID | 66795 |
UniProt ID | Q8R1P4 |
Cytogenetics | 13 C3 |
MW | 24.7 kDa |
Gene Summary | E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210437 | Atg10 (untagged) - Mouse autophagy-related 10 (yeast) (Atg10), (10ug) |
USD 330.00 |
|
MG218897 | Atg10 (tGFP-tagged) - Mouse autophagy-related 10 (yeast) (Atg10), (10ug) |
USD 530.00 |
|
MR218897L3 | Lenti ORF clone of Atg10 (Myc-DDK-tagged) - Mouse autophagy-related 10 (yeast) (Atg10) |
USD 630.00 |
|
MR218897L4 | Lenti ORF clone of Atg10 (mGFP-tagged) - Mouse autophagy-related 10 (yeast) (Atg10) |
USD 630.00 |
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